##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AviII       AvrII       BamHI       BanII       BbeI        
BbrPI       BcgI        BciVI       BclI        BglI        
BglII       BlnI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrFI       BssHII      BstAPI      
BstBI       BstEII      BstPI       BstUI       CfoI        
Cfr10I      ClaI        CpoI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HaeII       HhaI        Hin1I       HinP1I      KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    StuI        SwaI        TaqI        TthHB8I     
XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         1072  GTATAC               GT^[AC][GT]AC
AclI         2400  AACGTT               AA^CGTT
AflII        1677  CTTAAG               C^TTAAG
AlwNI         383  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          124  CCTCAGG              CC^T[ACGT]AGG
AspHI        2267  GAGCAC               G[AT]GC[AT]^C
AspI         1491  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1970  CCCGGG               C^[CT]CG[GA]G
BanI         2022  GGCACC               G^G[CT][GA]CC
BfrI         1677  CTTAAG               C^TTAAG
BlpI          713  GCTCAGC              GC^T[ACGT]AGC
BmyI         2267  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      713  GCTCAGC              GC^T[ACGT]AGC
BsaBI        2615  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      2267  GAGCAC               G[AT]GC[AT]^C
BsoBI        1970  CCCGGG               C^[CT]CG[GA]G
Bsp1286I     2267  GAGCAC               G[AGT]GC[ACT]^C
BspHI        1273  TCATGA               T^CATGA
BsrBI         435  CCGCTC               CCGCTC(-3/-3)
BssSI        2270  CACGAG               CACGAG(-5/-1)
Bst1107I     1072  GTATAC               GTA^TAC
BstXI        2138  CCAAAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1072  GTATAC               GTA^TAC
Bsu36I        124  CCTCAGG              CC^T[ACGT]AGG
CelII         713  GCTCAGC              GC^T[ACGT]AGC
DraIII       2747  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           79  CCATGG               C^C[GA][CT]GG
Eco81I        124  CCTCAGG              CC^T[ACGT]AGG
EcoRV          93  GATATC               GAT^ATC
HgaI          259  GACGC                GACGC(5/10)
HincII        899  GTTAAC               GT[CT]^[GA]AC
HindII        899  GTTAAC               GT[CT]^[GA]AC
HindIII      1190  AAGCTT               A^AGCTT
HpaI          899  GTTAAC               GTT^AAC
MaeII        2401  ACGT                 A^CGT
MamI         2615  GATCCAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI           79  CCATGG               C^CATGG
NdeI          376  CATATG               CA^TATG
NspI          429  ACATGC               [GA]CATG^[CT]
PflFI        1491  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     2400  AACGTT               AA^CGTT
RcaI         1273  TCATGA               T^CATGA
SapI         1092  GAAGAGC              GCTCTTC(1/4)
ScaI         2772  AGTACT               AGT^ACT
SmaI         1970  CCCGGG               CCC^GGG
SpeI          541  ACTAGT               A^CTAGT
Tth111I      1491  GACTTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI        1970  CCCGGG               C^CCGGG
XmaI         1970  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AvaII        1790  GGTCC                G^G[AT]CC
             1816  GGACC               
BalI         2084  TGGCCA               TGG^CCA
             2546  TGGCCA              
BbsI           67  GTCTTC               GAAGAC(2/6)
             1122  GTCTTC              
BbvCI        1993  CCTCAGC              CCTCAGC(-5/-2)
             2234  GCTGAGG             
BpmI          111  CTGGAG               CTGGAG(16/14)
              219  CTGGAG              
BpuAI          67  GTCTTC               GAAGAC(2/6)
             1122  GTCTTC              
Bsp1407I      675  TGTACA               T^GTACA
              948  TGTACA              
BsrGI         675  TGTACA               T^GTACA
              948  TGTACA              
BstF5I       1637  CATCC                GGATG(2/0)
             2515  CATCC               
DraII        1789  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1815  GGGACCT             
EaeI         2084  TGGCCA               [CT]^GGCC[GA]
             2546  TGGCCA              
EcoO109I     1789  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1815  GGGACCT             
EcoRI         180  GAATTC               G^AATTC
              561  GAATTC              
FokI         1637  CATCC                GGATG(9/13)
             2515  CATCC               
HapII        1971  CCGG                 C^CGG
             2039  CCGG                
HpaII        1971  CCGG                 C^CGG
             2039  CCGG                
MluNI        2084  TGGCCA               TGG^CCA
             2546  TGGCCA              
MscI         2084  TGGCCA               TGG^CCA
             2546  TGGCCA              
MslI          904  CATTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2796  CATATTTGTG          
MspA1I        716  CAGCTG               C[AC]G^C[GT]G
             2249  CAGCTG              
MspI         1971  CCGG                 C^CGG
             2039  CCGG                
PleI         1117  GAGTC                GAGTC(4/5)
             1908  GAGTC               
PpuMI        1789  AGGTCCT              [GA]G^G[AT]CC[CT]
             1815  GGGACCT             
PstI          247  CTGCAG               CTGCA^G
              528  CTGCAG              
PvuII         716  CAGCTG               CAG^CTG
             2249  CAGCTG              
SmlI         1630  CTCAAG               C^T[CT][GA]AG
             1677  CTTAAG              
SspBI         675  TGTACA               T^GTACA
              948  TGTACA              
SspI          802  AATATT               AAT^ATT
             2580  AATATT              
Tsp45I        385  GTGAC                ^GT[GC]AC
             1916  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AseI        AsnI        Asp700I     BbvI        
BcnI        BfaI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsrI        BssKI       
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        EarI        EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PshBI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       Van91I      
XhoII       XmnI