################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BamHI BanII BbeI BbrPI BcgI BciVI BclI BglI BglII BlnI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrDI BsrFI BssHII BstAPI BstBI BstEII BstPI BstUI CfoI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I StuI SwaI TaqI TthHB8I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1072 GTATAC GT^[AC][GT]AC AclI 2400 AACGTT AA^CGTT AflII 1677 CTTAAG C^TTAAG AlwNI 383 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 124 CCTCAGG CC^T[ACGT]AGG AspHI 2267 GAGCAC G[AT]GC[AT]^C AspI 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1970 CCCGGG C^[CT]CG[GA]G BanI 2022 GGCACC G^G[CT][GA]CC BfrI 1677 CTTAAG C^TTAAG BlpI 713 GCTCAGC GC^T[ACGT]AGC BmyI 2267 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 713 GCTCAGC GC^T[ACGT]AGC BsaBI 2615 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 2267 GAGCAC G[AT]GC[AT]^C BsoBI 1970 CCCGGG C^[CT]CG[GA]G Bsp1286I 2267 GAGCAC G[AGT]GC[ACT]^C BspHI 1273 TCATGA T^CATGA BsrBI 435 CCGCTC CCGCTC(-3/-3) BssSI 2270 CACGAG CACGAG(-5/-1) Bst1107I 1072 GTATAC GTA^TAC BstXI 2138 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1072 GTATAC GTA^TAC Bsu36I 124 CCTCAGG CC^T[ACGT]AGG CelII 713 GCTCAGC GC^T[ACGT]AGC DraIII 2747 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 79 CCATGG C^C[GA][CT]GG Eco81I 124 CCTCAGG CC^T[ACGT]AGG EcoRV 93 GATATC GAT^ATC HgaI 259 GACGC GACGC(5/10) HincII 899 GTTAAC GT[CT]^[GA]AC HindII 899 GTTAAC GT[CT]^[GA]AC HindIII 1190 AAGCTT A^AGCTT HpaI 899 GTTAAC GTT^AAC MaeII 2401 ACGT A^CGT MamI 2615 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 79 CCATGG C^CATGG NdeI 376 CATATG CA^TATG NspI 429 ACATGC [GA]CATG^[CT] PflFI 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2400 AACGTT AA^CGTT RcaI 1273 TCATGA T^CATGA SapI 1092 GAAGAGC GCTCTTC(1/4) ScaI 2772 AGTACT AGT^ACT SmaI 1970 CCCGGG CCC^GGG SpeI 541 ACTAGT A^CTAGT Tth111I 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1970 CCCGGG C^CCGGG XmaI 1970 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AvaII 1790 GGTCC G^G[AT]CC 1816 GGACC BalI 2084 TGGCCA TGG^CCA 2546 TGGCCA BbsI 67 GTCTTC GAAGAC(2/6) 1122 GTCTTC BbvCI 1993 CCTCAGC CCTCAGC(-5/-2) 2234 GCTGAGG BpmI 111 CTGGAG CTGGAG(16/14) 219 CTGGAG BpuAI 67 GTCTTC GAAGAC(2/6) 1122 GTCTTC Bsp1407I 675 TGTACA T^GTACA 948 TGTACA BsrGI 675 TGTACA T^GTACA 948 TGTACA BstF5I 1637 CATCC GGATG(2/0) 2515 CATCC DraII 1789 AGGTCCT [GA]G^G[ACGT]CC[CT] 1815 GGGACCT EaeI 2084 TGGCCA [CT]^GGCC[GA] 2546 TGGCCA EcoO109I 1789 AGGTCCT [GA]G^G[ACGT]CC[CT] 1815 GGGACCT EcoRI 180 GAATTC G^AATTC 561 GAATTC FokI 1637 CATCC GGATG(9/13) 2515 CATCC HapII 1971 CCGG C^CGG 2039 CCGG HpaII 1971 CCGG C^CGG 2039 CCGG MluNI 2084 TGGCCA TGG^CCA 2546 TGGCCA MscI 2084 TGGCCA TGG^CCA 2546 TGGCCA MslI 904 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2796 CATATTTGTG MspA1I 716 CAGCTG C[AC]G^C[GT]G 2249 CAGCTG MspI 1971 CCGG C^CGG 2039 CCGG PleI 1117 GAGTC GAGTC(4/5) 1908 GAGTC PpuMI 1789 AGGTCCT [GA]G^G[AT]CC[CT] 1815 GGGACCT PstI 247 CTGCAG CTGCA^G 528 CTGCAG PvuII 716 CAGCTG CAG^CTG 2249 CAGCTG SmlI 1630 CTCAAG C^T[CT][GA]AG 1677 CTTAAG SspBI 675 TGTACA T^GTACA 948 TGTACA SspI 802 AATATT AAT^ATT 2580 AATATT Tsp45I 385 GTGAC ^GT[GC]AC 1916 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I BbvI BcnI BfaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FauI Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I XhoII XmnI