##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BamHI BanII BbeI
BbrPI BcgI BciVI BclI BglI
BglII BlnI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstUI CfoI
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoT22I FbaI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I StuI SwaI TaqI TthHB8I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 1072 GTATAC GT^[AC][GT]AC
AclI 2400 AACGTT AA^CGTT
AflII 1677 CTTAAG C^TTAAG
AlwNI 383 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 124 CCTCAGG CC^T[ACGT]AGG
AspHI 2267 GAGCAC G[AT]GC[AT]^C
AspI 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1970 CCCGGG C^[CT]CG[GA]G
BanI 2022 GGCACC G^G[CT][GA]CC
BfrI 1677 CTTAAG C^TTAAG
BlpI 713 GCTCAGC GC^T[ACGT]AGC
BmyI 2267 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 713 GCTCAGC GC^T[ACGT]AGC
BsaBI 2615 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2267 GAGCAC G[AT]GC[AT]^C
BsoBI 1970 CCCGGG C^[CT]CG[GA]G
Bsp1286I 2267 GAGCAC G[AGT]GC[ACT]^C
BspHI 1273 TCATGA T^CATGA
BsrBI 435 CCGCTC CCGCTC(-3/-3)
BssSI 2270 CACGAG CACGAG(-5/-1)
Bst1107I 1072 GTATAC GTA^TAC
BstXI 2138 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1072 GTATAC GTA^TAC
Bsu36I 124 CCTCAGG CC^T[ACGT]AGG
CelII 713 GCTCAGC GC^T[ACGT]AGC
DraIII 2747 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 79 CCATGG C^C[GA][CT]GG
Eco81I 124 CCTCAGG CC^T[ACGT]AGG
EcoRV 93 GATATC GAT^ATC
HgaI 259 GACGC GACGC(5/10)
HincII 899 GTTAAC GT[CT]^[GA]AC
HindII 899 GTTAAC GT[CT]^[GA]AC
HindIII 1190 AAGCTT A^AGCTT
HpaI 899 GTTAAC GTT^AAC
MaeII 2401 ACGT A^CGT
MamI 2615 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 79 CCATGG C^CATGG
NdeI 376 CATATG CA^TATG
NspI 429 ACATGC [GA]CATG^[CT]
PflFI 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2400 AACGTT AA^CGTT
RcaI 1273 TCATGA T^CATGA
SapI 1092 GAAGAGC GCTCTTC(1/4)
ScaI 2772 AGTACT AGT^ACT
SmaI 1970 CCCGGG CCC^GGG
SpeI 541 ACTAGT A^CTAGT
Tth111I 1491 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1970 CCCGGG C^CCGGG
XmaI 1970 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaII 1790 GGTCC G^G[AT]CC
1816 GGACC
BalI 2084 TGGCCA TGG^CCA
2546 TGGCCA
BbsI 67 GTCTTC GAAGAC(2/6)
1122 GTCTTC
BbvCI 1993 CCTCAGC CCTCAGC(-5/-2)
2234 GCTGAGG
BpmI 111 CTGGAG CTGGAG(16/14)
219 CTGGAG
BpuAI 67 GTCTTC GAAGAC(2/6)
1122 GTCTTC
Bsp1407I 675 TGTACA T^GTACA
948 TGTACA
BsrGI 675 TGTACA T^GTACA
948 TGTACA
BstF5I 1637 CATCC GGATG(2/0)
2515 CATCC
DraII 1789 AGGTCCT [GA]G^G[ACGT]CC[CT]
1815 GGGACCT
EaeI 2084 TGGCCA [CT]^GGCC[GA]
2546 TGGCCA
EcoO109I 1789 AGGTCCT [GA]G^G[ACGT]CC[CT]
1815 GGGACCT
EcoRI 180 GAATTC G^AATTC
561 GAATTC
FokI 1637 CATCC GGATG(9/13)
2515 CATCC
HapII 1971 CCGG C^CGG
2039 CCGG
HpaII 1971 CCGG C^CGG
2039 CCGG
MluNI 2084 TGGCCA TGG^CCA
2546 TGGCCA
MscI 2084 TGGCCA TGG^CCA
2546 TGGCCA
MslI 904 CATTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2796 CATATTTGTG
MspA1I 716 CAGCTG C[AC]G^C[GT]G
2249 CAGCTG
MspI 1971 CCGG C^CGG
2039 CCGG
PleI 1117 GAGTC GAGTC(4/5)
1908 GAGTC
PpuMI 1789 AGGTCCT [GA]G^G[AT]CC[CT]
1815 GGGACCT
PstI 247 CTGCAG CTGCA^G
528 CTGCAG
PvuII 716 CAGCTG CAG^CTG
2249 CAGCTG
SmlI 1630 CTCAAG C^T[CT][GA]AG
1677 CTTAAG
SspBI 675 TGTACA T^GTACA
948 TGTACA
SspI 802 AATATT AAT^ATT
2580 AATATT
Tsp45I 385 GTGAC ^GT[GC]AC
1916 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I BbvI
BcnI BfaI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
FauI Fnu4HI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp509I TspRI Van91I
XhoII XmnI