##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AgeI AocI
Aor51HI AscI Asp718I AviII AvrII
BamHI BbrPI BbvCI BglII BlnI
BlpI Bpu1102I BsaI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII BssSI BstAPI BstBI Bsu36I
CelII Cfr10I ClaI CpoI DraI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI FseI FspI HgaI
HpaI KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SphI Sse8387I StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1879 GACGTC GACGT^C
AflII 3026 CTTAAG C^TTAAG
AhdI 1797 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 289 GTGCAC G^TGCAC
ApaI 1129 GGGCCC GGGCC^C
ApaLI 289 GTGCAC G^TGCAC
AseI 2280 ATTAAT AT^TAAT
AsnI 2280 ATTAAT AT^TAAT
AspEI 1797 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 289 GTGCAC G[AT]GC[AT]^C
AspI 1076 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 228 CCCGGG C^[CT]CG[GA]G
BcgI 808 CGACGATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2285 TGATCA T^GATCA
BfrI 3026 CTTAAG C^TTAAG
BglI 2415 GCCGCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 245 ACCGGA [AT]^CCGG[AT]
BseRI 778 CTCCTC GAGGAG(10/8)
BsiHKAI 289 GTGCAC G[AT]GC[AT]^C
BsoBI 228 CCCGGG C^[CT]CG[GA]G
BspHI 2189 TCATGA T^CATGA
BspMI 650 ACCTGC ACCTGC(4/8)
BsrBI 235 GAGCGG CCGCTC(-3/-3)
Bst1107I 2963 GTATAC GTA^TAC
BstZ17I 2963 GTATAC GTA^TAC
DraIII 880 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2175 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1797 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1162 ATGCAT ATGCA^T
FbaI 2285 TGATCA T^GATCA
HindIII 2778 AAGCTT A^AGCTT
MslI 1787 CATAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 497 CATATG CA^TATG
NheI 2138 GCTAGC G^CTAGC
NsiI 1162 ATGCAT ATGCA^T
PflFI 1076 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 842 GAGTC GAGTC(4/5)
PshBI 2280 ATTAAT AT^TAAT
RcaI 2189 TCATGA T^CATGA
ScaI 2015 AGTACT AGT^ACT
SmaI 228 CCCGGG CCC^GGG
SmlI 3026 CTTAAG C^T[CT][GA]AG
SnaBI 3151 TACGTA TAC^GTA
SpeI 1561 ACTAGT A^CTAGT
Tth111I 1076 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2882 TCTAGA T^CTAGA
XmaCI 228 CCCGGG C^CCGGG
XmaI 228 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 184 CGCG CG^CG
211 CGCG
AlwNI 1204 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
2713 CAGCAGCTG
Asp700I 1615 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1865 GAATGGATTC
BanII 1129 GGGCCC G[GA]GC[CT]^C
1220 GAGCCC
BciVI 552 GTATCC GTATCC(6/5)
1068 GTATCC
BsaAI 2597 CACGTA [CT]AC^GT[GA]
3151 TACGTA
BsaBI 446 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2628 GATGATGATC
BspLU11I 629 ACATGT A^CATGT
1972 ACATGT
BstEII 2702 GGTAACC G^GT[ACGT]ACC
2853 GGTTACC
BstPI 2702 GGTAACC G^GT[ACGT]ACC
2853 GGTTACC
BstUI 184 CGCG CG^CG
211 CGCG
BstXI 557 CCAGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCATAGCTGTGG
BstYI 451 AGATCC [GA]^GATC[CT]
1453 GGATCT
DsaI 171 CCATGG C^C[GA][CT]GG
1045 CCATGG
EarI 1515 GAAGAG CTCTTC(1/4)
2547 GAAGAG
EcoO65I 2702 GGTAACC G^GT[ACGT]ACC
2853 GGTTACC
EcoT14I 171 CCATGG C^C[AT][AT]GG
1045 CCATGG
Ksp632I 1515 GAAGAG CTCTTC(1/4)
2547 GAAGAG
MamI 446 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2628 GATGATGATC
MflI 451 AGATCC [GA]^GATC[CT]
1453 GGATCT
MvnI 184 CGCG CG^CG
211 CGCG
NcoI 171 CCATGG C^CATGG
1045 CCATGG
PpuMI 951 GGGACCC [GA]G^G[AT]CC[CT]
2551 AGGACCT
StyI 171 CCATGG C^C[AT][AT]GG
1045 CCATGG
XcmI 286 CCAGTGCACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
904 CCAGTGCGGCTCTGG
XhoII 451 AGATCC [GA]^GATC[CT]
1453 GGATCT
XmnI 1615 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1865 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AvaII
BalI BanI BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoRV FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I