##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BcgI BclI BlnI BlpI
Bpu1102I BsaI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BspHI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoT22I
FbaI FseI FspI HgaI HindIII
KspI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SfuI SgrAI SnaBI SpeI SphI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 268 GGTACC G^GTACC
AcyI 1562 GGCGCC G[GA]^CG[CT]C
AflII 898 CTTAAG C^TTAAG
Asp718I 268 GGTACC G^GTACC
BalI 2662 TGGCCA TGG^CCA
BbeI 1562 GGCGCC GGCGC^C
BbrPI 1064 CACGTG CAC^GTG
BbvCI 226 GCTGAGG CCTCAGC(-5/-2)
BfrI 898 CTTAAG C^TTAAG
BsaBI 505 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1562 GGCGCC G[GA]^CG[CT]C
BsaWI 2452 ACCGGA [AT]^CCGG[AT]
BsrFI 1051 GCCGGT [GA]^CCGG[CT]
CfoI 1563 GCGC GCG^C
Cfr10I 1051 GCCGGT [GA]^CCGG[CT]
DraIII 3145 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2059 CCTAGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1599 GAATTC G^AATTC
EcoRV 3460 GATATC GAT^ATC
HaeII 1562 GGCGCC [GA]GCGC^[CT]
HhaI 1563 GCGC GCG^C
Hin1I 1562 GGCGCC G[GA]^CG[CT]C
HinP1I 1563 GCGC G^CGC
HincII 2049 GTTAAC GT[CT]^[GA]AC
HindII 2049 GTTAAC GT[CT]^[GA]AC
HpaI 2049 GTTAAC GTT^AAC
KasI 1562 GGCGCC G^GCGCC
KpnI 268 GGTACC GGTAC^C
MamI 505 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2662 TGGCCA TGG^CCA
MscI 2662 TGGCCA TGG^CCA
NarI 1562 GGCGCC GG^CGCC
NdeI 21 CATATG CA^TATG
PaeR7I 749 CTCGAG C^TCGAG
PmaCI 1064 CACGTG CAC^GTG
PmlI 1064 CACGTG CAC^GTG
PpuMI 372 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 475 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 463 GAAGAGC GCTCTTC(1/4)
SbfI 1119 CCTGCAGG CCTGCA^GG
SfoI 1562 GGCGCC GGC^GCC
Sse8387I 1119 CCTGCAGG CCTGCA^GG
StuI 2626 AGGCCT AGG^CCT
XhoI 749 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1915 GTAGAC GT^[AC][GT]AC
2153 GTCTAC
AlwNI 1286 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1619 CAGCAGCTG
BglI 157 GCCGGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1132 GCCCTAGTGGC
BglII 87 AGATCT A^GATCT
584 AGATCT
BsaAI 1064 CACGTG [CT]AC^GT[GA]
3168 TACGTG
BsmI 3254 GAATGC GAATGC(1/-1)
2525 GCATTC
Bsp1407I 318 TGTACA T^GTACA
2130 TGTACA
BspLU11I 428 ACATGT A^CATGT
2637 ACATGT
BsrBI 799 GAGCGG CCGCTC(-3/-3)
1072 GAGCGG
BsrDI 26 GCAATG GCAATG(2/0)
416 GCAATG
BsrGI 318 TGTACA T^GTACA
2130 TGTACA
BstEII 1423 GGTGACC G^GT[ACGT]ACC
1652 GGTTACC
BstF5I 161 GGATG GGATG(2/0)
2671 GGATG
BstPI 1423 GGTGACC G^GT[ACGT]ACC
1652 GGTTACC
DraI 2319 TTTAAA TTT^AAA
3549 TTTAAA
EcoO65I 1423 GGTGACC G^GT[ACGT]ACC
1652 GGTTACC
FauI 842 CCCGC CCCGC(4/6)
3189 GCGGG
FokI 161 GGATG GGATG(9/13)
2671 GGATG
PstI 1120 CTGCAG CTGCA^G
2898 CTGCAG
ScaI 518 AGTACT AGT^ACT
1746 AGTACT
SexAI 2164 ACCTGGT A^CC[AT]GGT
3146 ACCAGGT
SmaI 1163 CCCGGG CCC^GGG
1388 CCCGGG
SspBI 318 TGTACA T^GTACA
2130 TGTACA
XcmI 1657 CCATGGGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3005 CCATATTAGAGATGG
XmaCI 1163 CCCGGG C^CCGGG
1388 CCCGGG
XmaI 1163 CCCGGG C^CCGGG
1388 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApaI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI