##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I FbaI
FseI FspI HgaI Hin1I KasI
KpnI KspI MaeII MamI MluI
MluNI MroI MscI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 1335 GTGCAC G^TGCAC
AlwNI 908 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1335 GTGCAC G^TGCAC
AseI 1787 ATTAAT AT^TAAT
AsnI 1787 ATTAAT AT^TAAT
AspHI 1335 GTGCAC G[AT]GC[AT]^C
BamHI 1471 GGATCC G^GATCC
BanII 1176 GAGCCC G[GA]GC[CT]^C
BbvCI 2692 GCTGAGG CCTCAGC(-5/-2)
BcnI 43 CCGGG CC^[GC]GG
BglII 1250 AGATCT A^GATCT
BsaJI 1070 CCACGG C^C[ACGT][ACGT]GG
BsgI 1171 GTGCAG GTGCAG(16/14)
BsiHKAI 1335 GTGCAC G[AT]GC[AT]^C
BsmBI 442 CGTCTC CGTCTC(1/5)
Bsp1407I 1809 TGTACA T^GTACA
BsrDI 2984 CATTGC GCAATG(2/0)
BsrGI 1809 TGTACA T^GTACA
DraIII 1644 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1070 CCACGG C^C[GA][CT]GG
FauI 32 CCCGC CCCGC(4/6)
HaeII 497 GGCGCT [GA]GCGC^[CT]
HapII 43 CCGG C^CGG
HindIII 1506 AAGCTT A^AGCTT
HpaI 1057 GTTAAC GTT^AAC
HpaII 43 CCGG C^CGG
MspA1I 164 CAGCTG C[AC]G^C[GT]G
MspI 43 CCGG C^CGG
NciI 43 CCGGG CC^[GC]GG
NdeI 2356 CATATG CA^TATG
PflMI 763 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 2980 GACTC GAGTC(4/5)
PmeI 2991 GTTTAAAC GTTT^AAAC
PshBI 1787 ATTAAT AT^TAAT
PvuII 164 CAGCTG CAG^CTG
ScaI 1399 AGTACT AGT^ACT
SexAI 424 ACCAGGT A^CC[AT]GGT
SspBI 1809 TGTACA T^GTACA
StuI 399 AGGCCT AGG^CCT
Tsp45I 800 GTGAC ^GT[GC]AC
Van91I 763 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1696 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 101 ACATGT A^C[GA][CT]GT
474 ACATGT
Asp700I 1248 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2497 GAATTACTTC
AvaII 1432 GGACC G^G[AT]CC
1573 GGTCC
BanI 1205 GGCACC G^G[CT][GA]CC
2323 GGCACC
BsaI 428 GGTCTC GGTCTC(1/5)
1627 GGTCTC
BspHI 522 TCATGA T^CATGA
1824 TCATGA
BspLU11I 101 ACATGT A^CATGT
474 ACATGT
BspMI 912 ACCTGC ACCTGC(4/8)
2847 ACCTGC
EcoRI 2351 GAATTC G^AATTC
2782 GAATTC
EcoT22I 974 ATGCAT ATGCA^T
2457 ATGCAT
HincII 1057 GTTAAC GT[CT]^[GA]AC
2977 GTTGAC
HindII 1057 GTTAAC GT[CT]^[GA]AC
2977 GTTGAC
MfeI 536 CAATTG C^AATTG
1182 CAATTG
MunI 536 CAATTG C^AATTG
1182 CAATTG
NsiI 974 ATGCAT ATGCA^T
2457 ATGCAT
PstI 2849 CTGCAG CTGCA^G
2950 CTGCAG
RcaI 522 TCATGA T^CATGA
1824 TCATGA
SapI 1276 GCTCTTC GCTCTTC(1/4)
2593 GCTCTTC
TfiI 813 GAATC G^A[AT]TC
1653 GAATC
XmnI 1248 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2497 GAATTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BciVI BfaI BmyI
BpmI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII