##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII BamHI BbeI BcgI
BciVI BclI BfrI BglII BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
CpoI DraI DrdI Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SwaI Tsp509I Tth111I XbaI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 1004 GGCGTC G[GA]^CG[CT]C
AhdI 1999 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1619 GTGCAC G^TGCAC
AlwI 321 GGATC GGATC(4/5)
AocI 83 CCTTAGG CC^T[ACGT]AGG
ApaLI 1619 GTGCAC G^TGCAC
AspEI 1999 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2689 CCTAGG C^CTAGG
BbrPI 1843 CACGTG CAC^GTG
BglI 1340 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2689 CCTAGG C^CTAGG
BsaAI 1843 CACGTG [CT]AC^GT[GA]
BsaHI 1004 GGCGTC G[GA]^CG[CT]C
BsaI 1305 GAGACC GGTCTC(1/5)
BsaWI 1040 ACCGGA [AT]^CCGG[AT]
BsgI 2074 CTGCAC GTGCAG(16/14)
BspHI 1650 TCATGA T^CATGA
BspLU11I 1707 ACATGT A^CATGT
BsrFI 932 GCCGGT [GA]^CCGG[CT]
BstAPI 2154 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 63 CCAAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 83 CCTTAGG CC^T[ACGT]AGG
Cfr10I 932 GCCGGT [GA]^CCGG[CT]
DraIII 1840 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1056 CGGCCG C^GGCCG
Eam1105I 1999 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1056 CGGCCG C^GGCCG
Eco52I 1056 CGGCCG C^GGCCG
Eco81I 83 CCTTAGG CC^T[ACGT]AGG
Hin1I 1004 GGCGTC G[GA]^CG[CT]C
HindIII 2483 AAGCTT A^AGCTT
PaeR7I 177 CTCGAG C^TCGAG
PmaCI 1843 CACGTG CAC^GTG
PmlI 1843 CACGTG CAC^GTG
PshAI 2666 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1650 TCATGA T^CATGA
SbfI 1402 CCTGCAGG CCTGCA^GG
Sse8387I 1402 CCTGCAGG CCTGCA^GG
SspI 1817 AATATT AAT^ATT
XhoI 177 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 1000 CGCG CG^CG
1491 CGCG
AflIII 1707 ACATGT A^C[GA][CT]GT
1844 ACGTGT
ApaI 798 GGGCCC GGGCC^C
1191 GGGCCC
AspHI 454 GAGCAC G[AT]GC[AT]^C
1619 GTGCAC
AvaI 177 CTCGAG C^[CT]CG[GA]G
1831 CTCGGG
BanI 2468 GGTGCC G^G[CT][GA]CC
2769 GGCACC
BbsI 1879 GAAGAC GAAGAC(2/6)
108 GTCTTC
BbvCI 448 GCTGAGG CCTCAGC(-5/-2)
1013 GCTGAGG
BpuAI 1879 GAAGAC GAAGAC(2/6)
108 GTCTTC
BsiEI 934 CGGTCG CG[GA][CT]^CG
1056 CGGCCG
BsiHKAI 454 GAGCAC G[AT]GC[AT]^C
1619 GTGCAC
BsoBI 177 CTCGAG C^[CT]CG[GA]G
1831 CTCGGG
BsrDI 1743 GCAATG GCAATG(2/0)
2232 GCAATG
BssSI 2024 CACGAG CACGAG(-5/-1)
2147 CACGAG
BstUI 1000 CGCG CG^CG
1491 CGCG
EcoNI 723 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2524 CCTTTGGGAGG
MvnI 1000 CGCG CG^CG
1491 CGCG
NspI 1688 GCATGT [GA]CATG^[CT]
1707 ACATGT
PstI 685 CTGCAG CTGCA^G
1403 CTGCAG
StuI 87 AGGCCT AGG^CCT
2725 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BalI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI