##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BcgI BclI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII BssSI BstBI
BstEII BstPI BstUI CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NarI NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SspI StuI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 2164 GAATTC [GA]^AATT[CT]
AflII 599 CTTAAG C^TTAAG
AlwNI 311 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 231 GGGCCC GGGCC^C
ApoI 2164 GAATTC [GA]^AATT[CT]
AseI 2295 ATTAAT AT^TAAT
AsnI 2295 ATTAAT AT^TAAT
AspI 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3173 GGATCC G^GATCC
BciVI 662 GTATCC GTATCC(6/5)
BfrI 599 CTTAAG C^TTAAG
BglII 399 AGATCT A^GATCT
BspHI 2168 TCATGA T^CATGA
BspMI 448 GCAGGT ACCTGC(4/8)
Bst1107I 2608 GTATAC GTA^TAC
BstAPI 448 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2608 GTATAC GTA^TAC
CfoI 532 GCGC GCG^C
DsaI 645 CCATGG C^C[GA][CT]GG
EcoRI 2164 GAATTC G^AATTC
EcoT22I 2954 ATGCAT ATGCA^T
HaeII 531 AGCGCC [GA]GCGC^[CT]
HhaI 532 GCGC GCG^C
HinP1I 532 GCGC G^CGC
HincII 906 GTCGAC GT[CT]^[GA]AC
HindII 906 GTCGAC GT[CT]^[GA]AC
NcoI 645 CCATGG C^CATGG
NdeI 2927 CATATG CA^TATG
NsiI 2954 ATGCAT ATGCA^T
NspI 2956 GCATGC [GA]CATG^[CT]
PflFI 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2295 ATTAAT AT^TAAT
RcaI 2168 TCATGA T^CATGA
SacI 365 GAGCTC GAGCT^C
SalI 906 GTCGAC G^TCGAC
ScaI 705 AGTACT AGT^ACT
SpeI 376 ACTAGT A^CTAGT
SphI 2956 GCATGC GCATG^C
Tth111I 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2201 TCTAGA T^CTAGA
XcmI 2500 CCAGAGGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 2364 TGGCCA TGG^CCA
2675 TGGCCA
BbvCI 163 CCTCAGC CCTCAGC(-5/-2)
2432 CCTCAGC
BsmI 1212 GAATGC GAATGC(1/-1)
1673 GAATGC
BsrBI 1128 CCGCTC CCGCTC(-3/-3)
1589 CCGCTC
BsrFI 1096 GCCGGC [GA]^CCGG[CT]
1557 GCCGGC
Cfr10I 1096 GCCGGC [GA]^CCGG[CT]
1557 GCCGGC
EaeI 2364 TGGCCA [CT]^GGCC[GA]
2675 TGGCCA
EcoNI 787 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2673 CCTGGCCAAGG
HgaI 807 GCGTC GACGC(5/10)
876 GCGTC
MaeII 1332 ACGT A^CGT
1793 ACGT
MluNI 2364 TGGCCA TGG^CCA
2675 TGGCCA
MscI 2364 TGGCCA TGG^CCA
2675 TGGCCA
MspA1I 1443 CAGCTG C[AC]G^C[GT]G
1904 CAGCTG
NaeI 1096 GCCGGC GCC^GGC
1557 GCCGGC
NgoMIV 1096 GCCGGC G^CCGGC
1557 GCCGGC
PpuMI 537 GGGACCT [GA]G^G[AT]CC[CT]
2423 AGGTCCC
PvuII 1443 CAGCTG CAG^CTG
1904 CAGCTG
SapI 416 GAAGAGC GCTCTTC(1/4)
440 GAAGAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AocI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI