################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AvaI AviII AvrII BanI BbeI BbrPI BcgI BclI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SspI StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcsI 2164 GAATTC [GA]^AATT[CT] AflII 599 CTTAAG C^TTAAG AlwNI 311 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 231 GGGCCC GGGCC^C ApoI 2164 GAATTC [GA]^AATT[CT] AseI 2295 ATTAAT AT^TAAT AsnI 2295 ATTAAT AT^TAAT AspI 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3173 GGATCC G^GATCC BciVI 662 GTATCC GTATCC(6/5) BfrI 599 CTTAAG C^TTAAG BglII 399 AGATCT A^GATCT BspHI 2168 TCATGA T^CATGA BspMI 448 GCAGGT ACCTGC(4/8) Bst1107I 2608 GTATAC GTA^TAC BstAPI 448 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2608 GTATAC GTA^TAC CfoI 532 GCGC GCG^C DsaI 645 CCATGG C^C[GA][CT]GG EcoRI 2164 GAATTC G^AATTC EcoT22I 2954 ATGCAT ATGCA^T HaeII 531 AGCGCC [GA]GCGC^[CT] HhaI 532 GCGC GCG^C HinP1I 532 GCGC G^CGC HincII 906 GTCGAC GT[CT]^[GA]AC HindII 906 GTCGAC GT[CT]^[GA]AC NcoI 645 CCATGG C^CATGG NdeI 2927 CATATG CA^TATG NsiI 2954 ATGCAT ATGCA^T NspI 2956 GCATGC [GA]CATG^[CT] PflFI 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 2295 ATTAAT AT^TAAT RcaI 2168 TCATGA T^CATGA SacI 365 GAGCTC GAGCT^C SalI 906 GTCGAC G^TCGAC ScaI 705 AGTACT AGT^ACT SpeI 376 ACTAGT A^CTAGT SphI 2956 GCATGC GCATG^C Tth111I 3079 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2201 TCTAGA T^CTAGA XcmI 2500 CCAGAGGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BalI 2364 TGGCCA TGG^CCA 2675 TGGCCA BbvCI 163 CCTCAGC CCTCAGC(-5/-2) 2432 CCTCAGC BsmI 1212 GAATGC GAATGC(1/-1) 1673 GAATGC BsrBI 1128 CCGCTC CCGCTC(-3/-3) 1589 CCGCTC BsrFI 1096 GCCGGC [GA]^CCGG[CT] 1557 GCCGGC Cfr10I 1096 GCCGGC [GA]^CCGG[CT] 1557 GCCGGC EaeI 2364 TGGCCA [CT]^GGCC[GA] 2675 TGGCCA EcoNI 787 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2673 CCTGGCCAAGG HgaI 807 GCGTC GACGC(5/10) 876 GCGTC MaeII 1332 ACGT A^CGT 1793 ACGT MluNI 2364 TGGCCA TGG^CCA 2675 TGGCCA MscI 2364 TGGCCA TGG^CCA 2675 TGGCCA MspA1I 1443 CAGCTG C[AC]G^C[GT]G 1904 CAGCTG NaeI 1096 GCCGGC GCC^GGC 1557 GCCGGC NgoMIV 1096 GCCGGC G^CCGGC 1557 GCCGGC PpuMI 537 GGGACCT [GA]G^G[AT]CC[CT] 2423 AGGTCCC PvuII 1443 CAGCTG CAG^CTG 1904 CAGCTG SapI 416 GAAGAGC GCTCTTC(1/4) 440 GAAGAGC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AocI Asp700I AspHI AvaII BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI