##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflII AgeI AhdI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1892 GACGTC GACGT^C
Alw44I 842 GTGCAC G^TGCAC
ApaLI 842 GTGCAC G^TGCAC
AspI 1577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1721 CACGTG CAC^GTG
BbsI 1882 GAAGAC GAAGAC(2/6)
BbvCI 2125 GCTGAGG CCTCAGC(-5/-2)
BpuAI 1882 GAAGAC GAAGAC(2/6)
BsaBI 2077 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 392 GAGACG CGTCTC(1/5)
BsmI 2627 GAATGC GAATGC(1/-1)
BspLU11I 521 ACATGT A^CATGT
BssSI 2169 CTCGTG CACGAG(-5/-1)
DraIII 190 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1208 GAAGAG CTCTTC(1/4)
FseI 896 GGCCGGCC GGCCGG^CC
HindIII 1859 AAGCTT A^AGCTT
Ksp632I 1208 GAAGAG CTCTTC(1/4)
MamI 2077 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 858 CCATGG C^CATGG
PflFI 1577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1721 CACGTG CAC^GTG
PmlI 1721 CACGTG CAC^GTG
SapI 1208 GAAGAGC GCTCTTC(1/4)
SmaI 2823 CCCGGG CCC^GGG
SnaBI 2558 TACGTA TAC^GTA
TfiI 343 GAATC G^A[AT]TC
Tsp509I 3232 AATT ^AATT
Tth111I 1577 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2823 CCCGGG C^CCGGG
XmaI 2823 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 1892 GACGTC G[GA]^CG[CT]C
3191 GGCGTC
AflIII 521 ACATGT A^C[GA][CT]GT
2045 ACGTGT
AocI 1078 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
Aor51HI 244 AGCGCT AGC^GCT
1521 AGCGCT
AspHI 370 GAGCAC G[AT]GC[AT]^C
842 GTGCAC
BalI 574 TGGCCA TGG^CCA
1803 TGGCCA
BpmI 2750 CTGGAG CTGGAG(16/14)
2915 CTGGAG
BsaAI 1721 CACGTG [CT]AC^GT[GA]
2558 TACGTA
BsaHI 1892 GACGTC G[GA]^CG[CT]C
3191 GGCGTC
BsiEI 1167 CGGCCG CG[GA][CT]^CG
3078 CGGCCG
BsiHKAI 370 GAGCAC G[AT]GC[AT]^C
842 GTGCAC
BsrBI 1769 CCGCTC CCGCTC(-3/-3)
1330 GAGCGG
BstAPI 16 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1835 GCATCGAGTGC
BstEII 853 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
BstPI 853 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
BstXI 967 CCATGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2218 CCACACCCCTGG
BstYI 1041 GGATCT [GA]^GATC[CT]
2827 GGATCT
Bsu36I 1078 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
EagI 1167 CGGCCG C^GGCCG
3078 CGGCCG
EclXI 1167 CGGCCG C^GGCCG
3078 CGGCCG
Eco47III 244 AGCGCT AGC^GCT
1521 AGCGCT
Eco52I 1167 CGGCCG C^GGCCG
3078 CGGCCG
Eco81I 1078 CCTGAGG CC^T[ACGT]AGG
2962 CCTCAGG
EcoNI 1503 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1687 CCTGGGCCAGG
EcoO65I 853 GGTCACC G^GT[ACGT]ACC
988 GGTCACC
HgaI 362 GACGC GACGC(5/10)
3192 GCGTC
Hin1I 1892 GACGTC G[GA]^CG[CT]C
3191 GGCGTC
MflI 1041 GGATCT [GA]^GATC[CT]
2827 GGATCT
MluNI 574 TGGCCA TGG^CCA
1803 TGGCCA
MscI 574 TGGCCA TGG^CCA
1803 TGGCCA
MseI 2272 TTAA T^TAA
3234 TTAA
NaeI 320 GCCGGC GCC^GGC
897 GCCGGC
NdeI 2468 CATATG CA^TATG
2782 CATATG
NgoMIV 320 GCCGGC G^CCGGC
897 GCCGGC
PvuII 1004 CAGCTG CAG^CTG
1025 CAGCTG
SbfI 375 CCTGCAGG CCTGCA^GG
1363 CCTGCAGG
SmlI 1679 CTCAAG C^T[CT][GA]AG
1898 CTCAAG
Sse8387I 375 CCTGCAGG CCTGCA^GG
1363 CCTGCAGG
Tru9I 2272 TTAA T^TAA
3234 TTAA
XcmI 348 CCAACCTCTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
447 CCAAGAGCTGCGTGG
XhoII 1041 GGATCT [GA]^GATC[CT]
2827 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AvaI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I