##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
Acc65I AclI AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AvrII BbeI BbvCI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
DraI DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HaeII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
PacI PinAI PmeI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1198 GACGTC GACGT^C
AccI 64 GTAGAC GT^[AC][GT]AC
AccII 1510 CGCG CG^CG
AccIII 726 TCCGGA T^CCGGA
AcyI 1198 GACGTC G[GA]^CG[CT]C
AflII 2644 CTTAAG C^TTAAG
AspI 438 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2159 TGCGCA TGC^GCA
BalI 3350 TGGCCA TGG^CCA
BamHI 267 GGATCC G^GATCC
BanI 1962 GGCACC G^G[CT][GA]CC
BciVI 3180 GTATCC GTATCC(6/5)
BfrI 2644 CTTAAG C^TTAAG
BglI 1302 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1198 GACGTC G[GA]^CG[CT]C
BseAI 726 TCCGGA T^CCGGA
BsiEI 503 CGGCCG CG[GA][CT]^CG
BsmBI 1050 CGTCTC CGTCTC(1/5)
BsmI 511 GAATGC GAATGC(1/-1)
BspEI 726 TCCGGA T^CCGGA
BspLU11I 2006 ACATGT A^CATGT
BsrBI 1552 GAGCGG CCGCTC(-3/-3)
BsrDI 1472 GCAATG GCAATG(2/0)
BsrFI 505 GCCGGC [GA]^CCGG[CT]
BssSI 720 CACGAG CACGAG(-5/-1)
BstAPI 1774 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2913 TTCGAA TT^CGAA
BstUI 1510 CGCG CG^CG
BstXI 1646 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 505 GCCGGC [GA]^CCGG[CT]
EagI 503 CGGCCG C^GGCCG
EclXI 503 CGGCCG C^GGCCG
Eco52I 503 CGGCCG C^GGCCG
FspI 2159 TGCGCA TGC^GCA
Hin1I 1198 GACGTC G[GA]^CG[CT]C
MluNI 3350 TGGCCA TGG^CCA
MroI 726 TCCGGA T^CCGGA
MscI 3350 TGGCCA TGG^CCA
MspA1I 1312 CCGCTG C[AC]G^C[GT]G
MvnI 1510 CGCG CG^CG
NaeI 505 GCCGGC GCC^GGC
NgoMIV 505 GCCGGC G^CCGGC
NspV 2913 TTCGAA TT^CGAA
PflFI 438 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3239 GAGCTC GAGCT^C
SapI 1686 GAAGAGC GCTCTTC(1/4)
SfuI 2913 TTCGAA TT^CGAA
SpeI 2675 ACTAGT A^CTAGT
SspI 1210 AATATT AAT^ATT
Tth111I 438 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 1383 GTAC GT^AC
1951 GTAC
AhdI 1198 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACATGTGGTC
Asp700I 1587 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3386 GAATCTTTTC
AspEI 1198 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACATGTGGTC
BspHI 1583 TCATGA T^CATGA
3214 TCATGA
BstEII 177 GGTCACC G^GT[ACGT]ACC
2662 GGTTACC
BstPI 177 GGTCACC G^GT[ACGT]ACC
2662 GGTTACC
BstYI 267 GGATCC [GA]^GATC[CT]
542 AGATCC
CfoI 1614 GCGC GCG^C
2160 GCGC
DraIII 598 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
1606 CACGTGGTG
DsaI 677 CCACGG C^C[GA][CT]GG
2512 CCGTGG
Eam1105I 1198 GACGTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2005 GACATGTGGTC
EarI 1252 CTCTTC CTCTTC(1/4)
1686 GAAGAG
EcoO65I 177 GGTCACC G^GT[ACGT]ACC
2662 GGTTACC
HgaI 924 GACGC GACGC(5/10)
1049 GCGTC
HhaI 1614 GCGC GCG^C
2160 GCGC
HinP1I 1614 GCGC G^CGC
2160 GCGC
HincII 1528 GTCAAC GT[CT]^[GA]AC
2212 GTCAAC
HindII 1528 GTCAAC GT[CT]^[GA]AC
2212 GTCAAC
Ksp632I 1252 CTCTTC CTCTTC(1/4)
1686 GAAGAG
MflI 267 GGATCC [GA]^GATC[CT]
542 AGATCC
NspI 2006 ACATGT [GA]CATG^[CT]
2162 GCATGT
PaeR7I 432 CTCGAG C^TCGAG
1409 CTCGAG
PshAI 171 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2128 GACAACTGTC
RcaI 1583 TCATGA T^CATGA
3214 TCATGA
RsaI 1383 GTAC GT^AC
1951 GTAC
SexAI 353 ACCTGGT A^CC[AT]GGT
439 ACCTGGT
XhoI 432 CTCGAG C^TCGAG
1409 CTCGAG
XhoII 267 GGATCC [GA]^GATC[CT]
542 AGATCC
XmnI 1587 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3386 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanII BbrPI BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PmaCI PmlI PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I