##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I AocI ApaLI
ApoI AseI AsnI Asp718I AvrII
BalI BamHI BbsI BbvCI BciVI
BclI BfrI BlnI BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI ScaI
SfiI SfuI SnaBI SspBI SspI
StuI SwaI TfiI Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 2549 GTCGAC GT^[AC][GT]AC
AhdI 2348 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1984 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 1748 GGCGCGCC GG^CGCGCC
Asp700I 2069 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2348 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2477 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2423 CACGTG CAC^GTG
BcgI 142 GCACCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3088 AGATCT A^GATCT
BlpI 952 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 952 GCTCAGC GC^T[ACGT]AGC
BsmI 2088 GAATGC GAATGC(1/-1)
BspLU11I 2859 ACATGT A^CATGT
BsrDI 1613 GCAATG GCAATG(2/0)
BstXI 1602 CCAAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 952 GCTCAGC GC^T[ACGT]AGC
DraI 232 TTTAAA TTT^AAA
Eam1105I 2348 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 2549 GTCGAC GT[CT]^[GA]AC
HindII 2549 GTCGAC GT[CT]^[GA]AC
PflFI 2477 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2423 CACGTG CAC^GTG
PmlI 2423 CACGTG CAC^GTG
SalI 2549 GTCGAC G^TCGAC
SbfI 2638 CCTGCAGG CCTGCA^GG
SexAI 2349 ACCAGGT A^CC[AT]GGT
SpeI 11 ACTAGT A^CTAGT
Sse8387I 2638 CCTGCAGG CCTGCA^GG
Tsp45I 491 GTCAC ^GT[GC]AC
Tth111I 2477 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2238 CCAACAACATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2069 GAAGCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AflIII 2545 ACACGT A^C[GA][CT]GT
2859 ACATGT
Aor51HI 591 AGCGCT AGC^GCT
1588 AGCGCT
AviII 2657 TGCGCA TGC^GCA
2739 TGCGCA
BsaAI 2423 CACGTG [CT]AC^GT[GA]
2672 TACGTG
BsgI 2420 CTGCAC GTGCAG(16/14)
2972 GTGCAG
BstAPI 2013 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2220 GCAGCCAGTGC
Eco47III 591 AGCGCT AGC^GCT
1588 AGCGCT
EcoT14I 2883 CCTTGG C^C[AT][AT]GG
2939 CCAAGG
FspI 2657 TGCGCA TGC^GCA
2739 TGCGCA
MaeIII 491 GTCAC ^GT[ACGT]AC
2827 GTTAC
PvuII 1627 CAGCTG CAG^CTG
2566 CAGCTG
SmlI 2036 CTCAAG C^T[CT][GA]AG
2534 CTCAAG
SphI 2229 GCATGC GCATG^C
2455 GCATGC
StyI 2883 CCTTGG C^C[AT][AT]GG
2939 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SgrAI
SmaI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI