##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI Asp718I
AspEI AviII AvrII BamHI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BspDI BstBI BstEII
BstPI CelII ClaI CpoI DraIII
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV FspI HindIII KpnI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SwaI Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccI 1119 GTATAC GT^[AC][GT]AC
AccIII 954 TCCGGA T^CCGGA
AflIII 3253 ACATGT A^C[GA][CT]GT
AocI 977 CCTCAGG CC^T[ACGT]AGG
ApaI 451 GGGCCC GGGCC^C
AspI 983 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2090 TGGCCA TGG^CCA
BbeI 526 GGCGCC GGCGC^C
BbrPI 150 CACGTG CAC^GTG
BclI 1344 TGATCA T^GATCA
BsaAI 150 CACGTG [CT]AC^GT[GA]
BsaI 2 GAGACC GGTCTC(1/5)
BseAI 954 TCCGGA T^CCGGA
BsiEI 736 CGGCCG CG[GA][CT]^CG
Bsp1407I 3103 TGTACA T^GTACA
BspEI 954 TCCGGA T^CCGGA
BspLU11I 3253 ACATGT A^CATGT
BspMI 1074 ACCTGC ACCTGC(4/8)
BsrGI 3103 TGTACA T^GTACA
BssSI 573 CTCGTG CACGAG(-5/-1)
Bst1107I 1119 GTATAC GTA^TAC
BstAPI 2047 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2429 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1119 GTATAC GTA^TAC
Bsu36I 977 CCTCAGG CC^T[ACGT]AGG
DraII 509 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 983 GACAGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 736 CGGCCG C^GGCCG
EclXI 736 CGGCCG C^GGCCG
Eco52I 736 CGGCCG C^GGCCG
Eco81I 977 CCTCAGG CC^T[ACGT]AGG
EcoO109I 509 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 2681 CCATGG C^C[AT][AT]GG
EcoT22I 1203 ATGCAT ATGCA^T
FbaI 1344 TGATCA T^GATCA
FseI 476 GGCCGGCC GGCCGG^CC
HincII 2424 GTTAAC GT[CT]^[GA]AC
HindII 2424 GTTAAC GT[CT]^[GA]AC
HpaI 2424 GTTAAC GTT^AAC
KasI 526 GGCGCC G^GCGCC
MluNI 2090 TGGCCA TGG^CCA
MroI 954 TCCGGA T^CCGGA
MscI 2090 TGGCCA TGG^CCA
NarI 526 GGCGCC GG^CGCC
NcoI 2681 CCATGG C^CATGG
NdeI 1348 CATATG CA^TATG
NsiI 1203 ATGCAT ATGCA^T
PflFI 983 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 150 CACGTG CAC^GTG
PmlI 150 CACGTG CAC^GTG
PpuMI 509 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 2057 CAGCTG CAG^CTG
ScaI 2507 AGTACT AGT^ACT
SfoI 526 GGCGCC GGC^GCC
SspBI 3103 TGTACA T^GTACA
StuI 195 AGGCCT AGG^CCT
StyI 2681 CCATGG C^C[AT][AT]GG
Tth111I 983 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1954 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 117 GACGCC G[GA]^CG[CT]C
526 GGCGCC
AlwNI 304 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
327 CAGCGACTG
AscI 136 GGCGCGCC GG^CGCGCC
702 GGCGCGCC
BanI 526 GGCGCC G^G[CT][GA]CC
751 GGCACC
BbvCI 6 CCTCAGC CCTCAGC(-5/-2)
2059 GCTGAGG
BciVI 882 GGATAC GTATCC(6/5)
1835 GGATAC
BglI 280 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
707 GCCGGGCGGGC
BsaHI 117 GACGCC G[GA]^CG[CT]C
526 GGCGCC
BsaWI 954 TCCGGA [AT]^CCGG[AT]
2084 ACCGGA
BsgI 291 CTGCAC GTGCAG(16/14)
1964 GTGCAG
BsmBI 115 GAGACG CGTCTC(1/5)
1577 GAGACG
BspHI 1134 TCATGA T^CATGA
1418 TCATGA
BsrDI 2670 CATTGC GCAATG(2/0)
1915 GCAATG
BssHII 137 GCGCGC G^CGCGC
703 GCGCGC
HgaI 117 GACGC GACGC(5/10)
682 GCGTC
Hin1I 117 GACGCC G[GA]^CG[CT]C
526 GGCGCC
HphI 1027 GGTGA GGTGA(8/7)
1621 GGTGA
MaeII 151 ACGT A^CGT
1006 ACGT
MslI 1303 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2430 CACTGTGGTG
NaeI 477 GCCGGC GCC^GGC
618 GCCGGC
NgoMIV 477 GCCGGC G^CCGGC
618 GCCGGC
PstI 324 CTGCAG CTGCA^G
2054 CTGCAG
RcaI 1134 TCATGA T^CATGA
1418 TCATGA
SgrAI 617 CGCCGGCG C[GA]^CCGG[CT]G
653 CGCCGGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DsaI EaeI EarI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I KspI MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI XmnI