##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI Alw44I Aor51HI ApaLI ApoI
AseI AsnI Asp718I AspI AviII
BamHI BclI BfrI BglII BsaBI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tsp509I Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 556 ACGTGT A^C[GA][CT]GT
AhdI 14 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 1403 GGCGCGCC GG^CGCGCC
AspEI 14 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2447 CCTAGG C^CTAGG
BbrPI 1057 CACGTG CAC^GTG
BbsI 3064 GAAGAC GAAGAC(2/6)
BbvCI 999 CCTCAGC CCTCAGC(-5/-2)
BcgI 1228 CGAGCCCGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2447 CCTAGG C^CTAGG
BpuAI 3064 GAAGAC GAAGAC(2/6)
BsaAI 1057 CACGTG [CT]AC^GT[GA]
BsrBI 2517 GAGCGG CCGCTC(-3/-3)
BsrFI 776 GCCGGC [GA]^CCGG[CT]
BssHII 1404 GCGCGC G^CGCGC
BstYI 995 AGATCC [GA]^GATC[CT]
Cfr10I 776 GCCGGC [GA]^CCGG[CT]
DrdI 667 GACACCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 14 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 775 GGCCGGCC GGCCGG^CC
HgaI 1427 GACGC GACGC(5/10)
MflI 995 AGATCC [GA]^GATC[CT]
NaeI 776 GCCGGC GCC^GGC
NdeI 843 CATATG CA^TATG
NgoMIV 776 GCCGGC G^CCGGC
NotI 528 GCGGCCGC GC^GGCCGC
NspI 1158 GCATGT [GA]CATG^[CT]
PmaCI 1057 CACGTG CAC^GTG
PmlI 1057 CACGTG CAC^GTG
PpuMI 1631 GGGTCCT [GA]G^G[AT]CC[CT]
SbfI 801 CCTGCAGG CCTGCA^GG
SfiI 1938 GGCCACAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2224 CTCAAG C^T[CT][GA]AG
Sse8387I 801 CCTGCAGG CCTGCA^GG
XhoII 995 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2440 GTCTAC GT^[AC][GT]AC
2594 GTAGAC
AccII 364 CGCG CG^CG
1405 CGCG
AfaI 1389 GTAC GT^AC
2170 GTAC
AlwI 996 GATCC GGATC(4/5)
953 GGATC
AocI 618 CCTTAGG CC^T[ACGT]AGG
1542 CCTGAGG
ApaI 304 GGGCCC GGGCC^C
1495 GGGCCC
Asp700I 714 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2920 GAACAACTTC
BbeI 318 GGCGCC GGCGC^C
820 GGCGCC
BciVI 35 GGATAC GTATCC(6/5)
1023 GGATAC
BsaI 2460 GAGACC GGTCTC(1/5)
119 GGTCTC
BsiEI 529 CGGCCG CG[GA][CT]^CG
774 CGGCCG
BsmI 2189 GAATGC GAATGC(1/-1)
2852 GCATTC
BstUI 364 CGCG CG^CG
1405 CGCG
Bsu36I 618 CCTTAGG CC^T[ACGT]AGG
1542 CCTGAGG
EagI 529 CGGCCG C^GGCCG
774 CGGCCG
EclXI 529 CGGCCG C^GGCCG
774 CGGCCG
Eco52I 529 CGGCCG C^GGCCG
774 CGGCCG
Eco81I 618 CCTTAGG CC^T[ACGT]AGG
1542 CCTGAGG
HaeII 318 GGCGCC [GA]GCGC^[CT]
820 GGCGCC
KasI 318 GGCGCC G^GCGCC
820 GGCGCC
MseI 2418 TTAA T^TAA
2935 TTAA
MvnI 364 CGCG CG^CG
1405 CGCG
NarI 318 GGCGCC GG^CGCC
820 GGCGCC
NheI 76 GCTAGC G^CTAGC
1449 GCTAGC
RsaI 1389 GTAC GT^AC
2170 GTAC
SacI 328 GAGCTC GAGCT^C
984 GAGCTC
SfoI 318 GGCGCC GGC^GCC
820 GGCGCC
SmaI 323 CCCGGG CCC^GGG
429 CCCGGG
StuI 297 AGGCCT AGG^CCT
1769 AGGCCT
TfiI 2151 GATTC G^A[AT]TC
2886 GATTC
Tru9I 2418 TTAA T^TAA
2935 TTAA
XmaCI 323 CCCGGG C^CCGGG
429 CCCGGG
XmaI 323 CCCGGG C^CCGGG
429 CCCGGG
XmnI 714 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2920 GAACAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccIII AciI AcyI AluI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MroI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI