##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AspI
AvrII BalI BamHI BbeI BfrI
BglII BlnI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KasI
MluI MluNI MroI MscI NarI
NdeI NheI NotI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1493 GGTACC G^GTACC
AcyI 278 GACGCC G[GA]^CG[CT]C
Asp700I 454 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1493 GGTACC G^GTACC
AviII 1262 TGCGCA TGC^GCA
BbrPI 971 CACGTG CAC^GTG
BbsI 556 GAAGAC GAAGAC(2/6)
BcgI 535 GCAGGCCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1356 GTATCC GTATCC(6/5)
BclI 3010 TGATCA T^GATCA
BlpI 221 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 221 GCTGAGC GC^T[ACGT]AGC
BpuAI 556 GAAGAC GAAGAC(2/6)
BsaAI 971 CACGTG [CT]AC^GT[GA]
BsaBI 913 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 278 GACGCC G[GA]^CG[CT]C
BsmBI 1675 GAGACG CGTCTC(1/5)
BspMI 2476 ACCTGC ACCTGC(4/8)
BsrDI 1192 CATTGC GCAATG(2/0)
BstXI 19 CCATTGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2786 AGATCC [GA]^GATC[CT]
CelII 221 GCTGAGC GC^T[ACGT]AGC
DraI 3191 TTTAAA TTT^AAA
EcoNI 1852 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3010 TGATCA T^GATCA
FspI 1262 TGCGCA TGC^GCA
HgaI 278 GACGC GACGC(5/10)
Hin1I 278 GACGCC G[GA]^CG[CT]C
KpnI 1493 GGTACC GGTAC^C
MamI 913 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 837 CAATTG C^AATTG
MflI 2786 AGATCC [GA]^GATC[CT]
MunI 837 CAATTG C^AATTG
NruI 166 TCGCGA TCG^CGA
NspI 1453 ACATGC [GA]CATG^[CT]
PaeR7I 198 CTCGAG C^TCGAG
PflMI 1835 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 971 CACGTG CAC^GTG
PmlI 971 CACGTG CAC^GTG
SacI 272 GAGCTC GAGCT^C
ScaI 2607 AGTACT AGT^ACT
SspI 440 AATATT AAT^ATT
Van91I 1835 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1497 CCAATGCCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 198 CTCGAG C^TCGAG
XhoII 2786 AGATCC [GA]^GATC[CT]
XmnI 454 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 952 GTCTAC GT^[AC][GT]AC
3142 GTCTAC
AcsI 3117 AAATTC [GA]^AATT[CT]
3266 GAATTT
AlwI 1573 GATCC GGATC(4/5)
2787 GATCC
Aor51HI 1953 AGCGCT AGC^GCT
2013 AGCGCT
ApaI 29 GGGCCC GGGCC^C
123 GGGCCC
ApoI 3117 AAATTC [GA]^AATT[CT]
3266 GAATTT
BanI 1364 GGCACC G^G[CT][GA]CC
1493 GGTACC
BbvCI 716 CCTCAGC CCTCAGC(-5/-2)
2558 CCTCAGC
BglI 370 GCCGCCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
619 GCCAGACAGGC
DraIII 1585 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2679 CACAGGGTG
EaeI 1555 TGGCCG [CT]^GGCC[GA]
2630 TGGCCG
Eco47III 1953 AGCGCT AGC^GCT
2013 AGCGCT
HindIII 603 AAGCTT A^AGCTT
3442 AAGCTT
KspI 184 CCGCGG CCGC^GG
1990 CCGCGG
NaeI 1536 GCCGGC GCC^GGC
2737 GCCGGC
NcoI 132 CCATGG C^CATGG
432 CCATGG
NgoMIV 1536 GCCGGC G^CCGGC
2737 GCCGGC
PpuMI 1740 AGGACCT [GA]G^G[AT]CC[CT]
2029 AGGACCC
SacII 184 CCGCGG CCGC^GG
1990 CCGCGG
SapI 1022 GAAGAGC GCTCTTC(1/4)
3345 GAAGAGC
SfaNI 1516 GATGC GCATC(5/9)
3412 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmaCI XmaI