##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI AseI AsnI Asp718I
AspI AvrII BalI BclI BfrI
BlnI BseAI BsiWI BspDI BspEI
BspLU11I Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MluI MluNI MroI MscI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI StuI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 613 GTCGAC GT^[AC][GT]AC
AocI 3099 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2481 AGCGCT AGC^GCT
BlpI 766 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 766 GCTGAGC GC^T[ACGT]AGC
BsaBI 2325 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2143 TCCGGT [AT]^CCGG[AT]
BsmI 161 GAATGC GAATGC(1/-1)
Bsp1407I 2825 TGTACA T^GTACA
BspHI 2450 TCATGA T^CATGA
BsrGI 2825 TGTACA T^GTACA
BssSI 1566 CTCGTG CACGAG(-5/-1)
BstEII 2570 GGTGACC G^GT[ACGT]ACC
BstPI 2570 GGTGACC G^GT[ACGT]ACC
Bsu36I 3099 CCTCAGG CC^T[ACGT]AGG
CelII 766 GCTGAGC GC^T[ACGT]AGC
Eco47III 2481 AGCGCT AGC^GCT
Eco81I 3099 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2570 GGTGACC G^GT[ACGT]ACC
EcoRI 3518 GAATTC G^AATTC
KspI 1716 CCGCGG CCGC^GG
MamI 2325 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3126 CAATTG C^AATTG
MunI 3126 CAATTG C^AATTG
PaeR7I 2296 CTCGAG C^TCGAG
PshAI 606 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2450 TCATGA T^CATGA
SacI 2854 GAGCTC GAGCT^C
SacII 1716 CCGCGG CCGC^GG
SalI 613 GTCGAC G^TCGAC
SbfI 3192 CCTGCAGG CCTGCA^GG
Sse8387I 3192 CCTGCAGG CCTGCA^GG
SspBI 2825 TGTACA T^GTACA
XhoI 2296 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AcsI 436 GAATTT [GA]^AATT[CT]
3518 GAATTC
AflIII 1901 ACACGT A^C[GA][CT]GT
3051 ACGTGT
AhdI 985 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1099 GACATTTTGTC
Alw44I 1823 GTGCAC G^TGCAC
1963 GTGCAC
ApaLI 1823 GTGCAC G^TGCAC
1963 GTGCAC
ApoI 436 GAATTT [GA]^AATT[CT]
3518 GAATTC
Asp700I 1668 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2358 GAAGCCCTTC
AspEI 985 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1099 GACATTTTGTC
BbrPI 1902 CACGTG CAC^GTG
3050 CACGTG
BbvCI 40 CCTCAGC CCTCAGC(-5/-2)
2104 CCTCAGC
BciVI 3165 GGATAC GTATCC(6/5)
3541 GTATCC
BglII 296 AGATCT A^GATCT
2182 AGATCT
BsaAI 1902 CACGTG [CT]AC^GT[GA]
3050 CACGTG
BsaI 2695 GGTCTC GGTCTC(1/5)
2713 GGTCTC
BsmBI 2398 GAGACG CGTCTC(1/5)
2597 GAGACG
BsrDI 618 CATTGC GCAATG(2/0)
2135 CATTGC
BsrFI 132 GCCGGC [GA]^CCGG[CT]
3420 GCCGGC
BssHII 2627 GCGCGC G^CGCGC
2629 GCGCGC
BstAPI 1502 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1854 GCAGAACCTGC
BstXI 1132 CCATACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1307 CCATCGCGGTGG
Cfr10I 132 GCCGGC [GA]^CCGG[CT]
3420 GCCGGC
DraIII 900 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3314 CACCGGGTG
EagI 31 CGGCCG C^GGCCG
428 CGGCCG
Eam1105I 985 GACCTCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1099 GACATTTTGTC
EclXI 31 CGGCCG C^GGCCG
428 CGGCCG
Eco52I 31 CGGCCG C^GGCCG
428 CGGCCG
EcoNI 480 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
687 CCTTGTTAAGG
HincII 24 GTCAAC GT[CT]^[GA]AC
613 GTCGAC
HindII 24 GTCAAC GT[CT]^[GA]AC
613 GTCGAC
NaeI 132 GCCGGC GCC^GGC
3420 GCCGGC
NcoI 1138 CCATGG C^CATGG
2848 CCATGG
NgoMIV 132 GCCGGC G^CCGGC
3420 GCCGGC
PmaCI 1902 CACGTG CAC^GTG
3050 CACGTG
PmlI 1902 CACGTG CAC^GTG
3050 CACGTG
SapI 1668 GAAGAGC GCTCTTC(1/4)
2239 GAAGAGC
TaqI 614 TCGA T^CGA
2297 TCGA
TfiI 952 GAATC G^A[AT]TC
3221 GATTC
TthHB8I 614 TCGA T^CGA
2297 TCGA
XcmI 323 CCATTCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2519 CCATTTCACCCTTGG
XmnI 1668 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2358 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BamHI BanI BanII
BbeI BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI