##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BamHI BbeI BbsI
BbvCI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaI BsiWI
BsmBI BspDI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRV EcoT22I FbaI
FspI HindIII HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1950 GTCTAC GT^[AC][GT]AC
AccIII 1333 TCCGGA T^CCGGA
AcsI 2577 GAATTC [GA]^AATT[CT]
AcyI 2595 GGCGTC G[GA]^CG[CT]C
AocI 1482 CCTGAGG CC^T[ACGT]AGG
ApaI 2415 GGGCCC GGGCC^C
ApoI 2577 GAATTC [GA]^AATT[CT]
BbrPI 3273 CACGTG CAC^GTG
BcgI 2120 GCAGTGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 3273 CACGTG [CT]AC^GT[GA]
BsaHI 2595 GGCGTC G[GA]^CG[CT]C
BseAI 1333 TCCGGA T^CCGGA
BsmI 1280 GAATGC GAATGC(1/-1)
BspEI 1333 TCCGGA T^CCGGA
BspHI 1411 TCATGA T^CATGA
Bsu36I 1482 CCTGAGG CC^T[ACGT]AGG
DraI 1092 TTTAAA TTT^AAA
DraIII 832 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1488 GACAAAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1482 CCTGAGG CC^T[ACGT]AGG
EcoRI 2577 GAATTC G^AATTC
FseI 1215 GGCCGGCC GGCCGG^CC
HgaI 2596 GCGTC GACGC(5/10)
Hin1I 2595 GGCGTC G[GA]^CG[CT]C
MroI 1333 TCCGGA T^CCGGA
NaeI 1216 GCCGGC GCC^GGC
NcoI 976 CCATGG C^CATGG
NgoMIV 1216 GCCGGC G^CCGGC
NspI 1927 GCATGT [GA]CATG^[CT]
PaeR7I 1285 CTCGAG C^TCGAG
PflMI 662 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3273 CACGTG CAC^GTG
PmlI 3273 CACGTG CAC^GTG
PshAI 2652 GACAGTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1411 TCATGA T^CATGA
SapI 776 GCTCTTC GCTCTTC(1/4)
SmaI 168 CCCGGG CCC^GGG
Van91I 662 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2660 TCTAGA T^CTAGA
XhoI 1285 CTCGAG C^TCGAG
XmaCI 168 CCCGGG C^CCGGG
XmaI 168 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BlpI 591 GCTGAGC GC^T[ACGT]AGC
1206 GCTCAGC
Bpu1102I 591 GCTGAGC GC^T[ACGT]AGC
1206 GCTCAGC
BsaWI 1333 TCCGGA [AT]^CCGG[AT]
1957 ACCGGA
BsiEI 45 CGGCCG CG[GA][CT]^CG
122 CGGCCG
BsmAI 927 GTCTC GTCTC(1/5)
1477 GTCTC
Bsp1407I 1930 TGTACA T^GTACA
2911 TGTACA
BsrGI 1930 TGTACA T^GTACA
2911 TGTACA
BstEII 854 GGTTACC G^GT[ACGT]ACC
878 GGTTACC
BstPI 854 GGTTACC G^GT[ACGT]ACC
878 GGTTACC
BstYI 1361 GGATCT [GA]^GATC[CT]
2602 AGATCC
CelII 591 GCTGAGC GC^T[ACGT]AGC
1206 GCTCAGC
EagI 45 CGGCCG C^GGCCG
122 CGGCCG
EarI 777 CTCTTC CTCTTC(1/4)
2148 CTCTTC
EclXI 45 CGGCCG C^GGCCG
122 CGGCCG
Eco52I 45 CGGCCG C^GGCCG
122 CGGCCG
EcoO65I 854 GGTTACC G^GT[ACGT]ACC
878 GGTTACC
HincII 2172 GTCAAC GT[CT]^[GA]AC
2451 GTCAAC
HindII 2172 GTCAAC GT[CT]^[GA]AC
2451 GTCAAC
Ksp632I 777 CTCTTC CTCTTC(1/4)
2148 CTCTTC
MflI 1361 GGATCT [GA]^GATC[CT]
2602 AGATCC
MslI 723 CACAACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1928 CATGTACATG
NdeI 1345 CATATG CA^TATG
2757 CATATG
SacI 594 GAGCTC GAGCT^C
2667 GAGCTC
ScaI 2335 AGTACT AGT^ACT
3022 AGTACT
SfiI 420 GGCCTGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1219 GGCCACCTCGGCC
SspBI 1930 TGTACA T^GTACA
2911 TGTACA
Tsp509I 2578 AATT ^AATT
3136 AATT
XhoII 1361 GGATCT [GA]^GATC[CT]
2602 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AspI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI Tth111I TthHB8I
XcmI XmnI