##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbvCI       BciVI       BlnI        BlpI        Bpu1102I    
BsaBI       BseAI       BsiEI       BsiWI       BsmI        
BspDI       BspEI       BssHII      Bst1107I    BstAPI      
BstUI       BstZ17I     CelII       ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoT22I     FseI        FspI        
HpaI        KasI        KpnI        KspI        MamI        
MluI        MroI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PflFI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcyI         1661  GACGCC               G[GA]^CG[CT]C
Alw44I        365  GTGCAC               G^TGCAC
AocI         3215  CCTGAGG              CC^T[ACGT]AGG
ApaI         2244  GGGCCC               GGGCC^C
ApaLI         365  GTGCAC               G^TGCAC
AseI          845  ATTAAT               AT^TAAT
AsnI          845  ATTAAT               AT^TAAT
Asp700I      3295  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         405  GGATCC               G^GATCC
BbrPI        1746  CACGTG               CAC^GTG
BcgI         2416  GCATAAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          633  TGATCA               T^GATCA
BcnI         1862  CCGGG                CC^[GC]GG
BglI         2847  GCCAGCGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1661  GACGCC               G[GA]^CG[CT]C
BsaWI        1253  ACCGGA               [AT]^CCGG[AT]
BsmBI        1484  GAGACG               CGTCTC(1/5)
BspHI        2746  TCATGA               T^CATGA
BspLU11I     2705  ACATGT               A^CATGT
BsrBI        3277  CCGCTC               CCGCTC(-3/-3)
BsrDI        3246  CATTGC               GCAATG(2/0)
BsrFI        1760  ACCGGC               [GA]^CCGG[CT]
BstBI        3261  TTCGAA               TT^CGAA
Bsu36I       3215  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       1760  ACCGGC               [GA]^CCGG[CT]
Eco81I       3215  CCTGAGG              CC^T[ACGT]AGG
EcoNI         284  CCTCTTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        1221  GATATC               GAT^ATC
EcoT14I      1242  CCAAGG               C^C[AT][AT]GG
FbaI          633  TGATCA               T^GATCA
HgaI         1661  GACGC                GACGC(5/10)
Hin1I        1661  GACGCC               G[GA]^CG[CT]C
HincII       2456  GTCAAC               GT[CT]^[GA]AC
HindII       2456  GTCAAC               GT[CT]^[GA]AC
HindIII      3676  AAGCTT               A^AGCTT
MfeI         2979  CAATTG               C^AATTG
MunI         2979  CAATTG               C^AATTG
NciI         1862  CCGGG                CC^[GC]GG
NdeI         3334  CATATG               CA^TATG
NspV         3261  TTCGAA               TT^CGAA
PmaCI        1746  CACGTG               CAC^GTG
PmlI         1746  CACGTG               CAC^GTG
PpuMI         594  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         845  ATTAAT               AT^TAAT
RcaI         2746  TCATGA               T^CATGA
SacI         1642  GAGCTC               GAGCT^C
SapI         1909  GAAGAGC              GCTCTTC(1/4)
ScaI         3491  AGTACT               AGT^ACT
SfuI         3261  TTCGAA               TT^CGAA
StuI         2356  AGGCCT               AGG^CCT
StyI         1242  CCAAGG               C^C[AT][AT]GG
XbaI         2618  TCTAGA               T^CTAGA
XmnI         3295  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          260  GTCTAC               GT^[AC][GT]AC
              739  GTCTAC              
AflIII       2705  ACATGT               A^C[GA][CT]GT
             2756  ACGTGT              
BglII        1608  AGATCT               A^GATCT
             1995  AGATCT              
BsaAI        1746  CACGTG               [CT]AC^GT[GA]
             2783  CACGTA              
Bsp1407I     1617  TGTACA               T^GTACA
             3004  TGTACA              
BsrGI        1617  TGTACA               T^GTACA
             3004  TGTACA              
BssSI        1428  CTCGTG               CACGAG(-5/-1)
             2488  CTCGTG              
BstEII       1234  GGTGACC              G^GT[ACGT]ACC
             1960  GGTAACC             
BstPI        1234  GGTGACC              G^GT[ACGT]ACC
             1960  GGTAACC             
CfoI         1185  GCGC                 GCG^C
             2860  GCGC                
DraII         594  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2244  GGGCCCC             
EarI         1909  GAAGAG               CTCTTC(1/4)
             2429  GAAGAG              
EcoO109I      594  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2244  GGGCCCC             
EcoO65I      1234  GGTGACC              G^GT[ACGT]ACC
             1960  GGTAACC             
EcoRI         469  GAATTC               G^AATTC
             2729  GAATTC              
HaeII        1184  AGCGCC               [GA]GCGC^[CT]
             2859  GGCGCT              
HhaI         1185  GCGC                 GCG^C
             2860  GCGC                
HinP1I       1185  GCGC                 G^CGC
             2860  GCGC                
Ksp632I      1909  GAAGAG               CTCTTC(1/4)
             2429  GAAGAG              
PaeR7I        381  CTCGAG               C^TCGAG
              441  CTCGAG              
PstI         1005  CTGCAG               CTGCA^G
             1119  CTGCAG              
PvuII          33  CAGCTG               CAG^CTG
              637  CAGCTG              
SspBI        1617  TGTACA               T^GTACA
             3004  TGTACA              
XcmI         1716  CCAATTCCTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2515  CCATTATGCCGTTGG     
XhoI          381  CTCGAG               C^TCGAG
              441  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvI        BfaI        BfrI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DsaI        
EaeI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII