################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII AvrII BbeI BbvCI BciVI BlnI BlpI Bpu1102I BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BssHII Bst1107I BstAPI BstUI BstZ17I CelII ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 1661 GACGCC G[GA]^CG[CT]C Alw44I 365 GTGCAC G^TGCAC AocI 3215 CCTGAGG CC^T[ACGT]AGG ApaI 2244 GGGCCC GGGCC^C ApaLI 365 GTGCAC G^TGCAC AseI 845 ATTAAT AT^TAAT AsnI 845 ATTAAT AT^TAAT Asp700I 3295 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 405 GGATCC G^GATCC BbrPI 1746 CACGTG CAC^GTG BcgI 2416 GCATAAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 633 TGATCA T^GATCA BcnI 1862 CCGGG CC^[GC]GG BglI 2847 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1661 GACGCC G[GA]^CG[CT]C BsaWI 1253 ACCGGA [AT]^CCGG[AT] BsmBI 1484 GAGACG CGTCTC(1/5) BspHI 2746 TCATGA T^CATGA BspLU11I 2705 ACATGT A^CATGT BsrBI 3277 CCGCTC CCGCTC(-3/-3) BsrDI 3246 CATTGC GCAATG(2/0) BsrFI 1760 ACCGGC [GA]^CCGG[CT] BstBI 3261 TTCGAA TT^CGAA Bsu36I 3215 CCTGAGG CC^T[ACGT]AGG Cfr10I 1760 ACCGGC [GA]^CCGG[CT] Eco81I 3215 CCTGAGG CC^T[ACGT]AGG EcoNI 284 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1221 GATATC GAT^ATC EcoT14I 1242 CCAAGG C^C[AT][AT]GG FbaI 633 TGATCA T^GATCA HgaI 1661 GACGC GACGC(5/10) Hin1I 1661 GACGCC G[GA]^CG[CT]C HincII 2456 GTCAAC GT[CT]^[GA]AC HindII 2456 GTCAAC GT[CT]^[GA]AC HindIII 3676 AAGCTT A^AGCTT MfeI 2979 CAATTG C^AATTG MunI 2979 CAATTG C^AATTG NciI 1862 CCGGG CC^[GC]GG NdeI 3334 CATATG CA^TATG NspV 3261 TTCGAA TT^CGAA PmaCI 1746 CACGTG CAC^GTG PmlI 1746 CACGTG CAC^GTG PpuMI 594 GGGACCT [GA]G^G[AT]CC[CT] PshBI 845 ATTAAT AT^TAAT RcaI 2746 TCATGA T^CATGA SacI 1642 GAGCTC GAGCT^C SapI 1909 GAAGAGC GCTCTTC(1/4) ScaI 3491 AGTACT AGT^ACT SfuI 3261 TTCGAA TT^CGAA StuI 2356 AGGCCT AGG^CCT StyI 1242 CCAAGG C^C[AT][AT]GG XbaI 2618 TCTAGA T^CTAGA XmnI 3295 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 260 GTCTAC GT^[AC][GT]AC 739 GTCTAC AflIII 2705 ACATGT A^C[GA][CT]GT 2756 ACGTGT BglII 1608 AGATCT A^GATCT 1995 AGATCT BsaAI 1746 CACGTG [CT]AC^GT[GA] 2783 CACGTA Bsp1407I 1617 TGTACA T^GTACA 3004 TGTACA BsrGI 1617 TGTACA T^GTACA 3004 TGTACA BssSI 1428 CTCGTG CACGAG(-5/-1) 2488 CTCGTG BstEII 1234 GGTGACC G^GT[ACGT]ACC 1960 GGTAACC BstPI 1234 GGTGACC G^GT[ACGT]ACC 1960 GGTAACC CfoI 1185 GCGC GCG^C 2860 GCGC DraII 594 GGGACCT [GA]G^G[ACGT]CC[CT] 2244 GGGCCCC EarI 1909 GAAGAG CTCTTC(1/4) 2429 GAAGAG EcoO109I 594 GGGACCT [GA]G^G[ACGT]CC[CT] 2244 GGGCCCC EcoO65I 1234 GGTGACC G^GT[ACGT]ACC 1960 GGTAACC EcoRI 469 GAATTC G^AATTC 2729 GAATTC HaeII 1184 AGCGCC [GA]GCGC^[CT] 2859 GGCGCT HhaI 1185 GCGC GCG^C 2860 GCGC HinP1I 1185 GCGC G^CGC 2860 GCGC Ksp632I 1909 GAAGAG CTCTTC(1/4) 2429 GAAGAG PaeR7I 381 CTCGAG C^TCGAG 441 CTCGAG PstI 1005 CTGCAG CTGCA^G 1119 CTGCAG PvuII 33 CAGCTG CAG^CTG 637 CAGCTG SspBI 1617 TGTACA T^GTACA 3004 TGTACA XcmI 1716 CCAATTCCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2515 CCATTATGCCGTTGG XhoI 381 CTCGAG C^TCGAG 441 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AflII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII