##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BbeI
BbvCI BciVI BlnI BlpI Bpu1102I
BsaBI BseAI BsiEI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstAPI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FseI FspI
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 1661 GACGCC G[GA]^CG[CT]C
Alw44I 365 GTGCAC G^TGCAC
AocI 3215 CCTGAGG CC^T[ACGT]AGG
ApaI 2244 GGGCCC GGGCC^C
ApaLI 365 GTGCAC G^TGCAC
AseI 845 ATTAAT AT^TAAT
AsnI 845 ATTAAT AT^TAAT
Asp700I 3295 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 405 GGATCC G^GATCC
BbrPI 1746 CACGTG CAC^GTG
BcgI 2416 GCATAAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 633 TGATCA T^GATCA
BcnI 1862 CCGGG CC^[GC]GG
BglI 2847 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1661 GACGCC G[GA]^CG[CT]C
BsaWI 1253 ACCGGA [AT]^CCGG[AT]
BsmBI 1484 GAGACG CGTCTC(1/5)
BspHI 2746 TCATGA T^CATGA
BspLU11I 2705 ACATGT A^CATGT
BsrBI 3277 CCGCTC CCGCTC(-3/-3)
BsrDI 3246 CATTGC GCAATG(2/0)
BsrFI 1760 ACCGGC [GA]^CCGG[CT]
BstBI 3261 TTCGAA TT^CGAA
Bsu36I 3215 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1760 ACCGGC [GA]^CCGG[CT]
Eco81I 3215 CCTGAGG CC^T[ACGT]AGG
EcoNI 284 CCTCTTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1221 GATATC GAT^ATC
EcoT14I 1242 CCAAGG C^C[AT][AT]GG
FbaI 633 TGATCA T^GATCA
HgaI 1661 GACGC GACGC(5/10)
Hin1I 1661 GACGCC G[GA]^CG[CT]C
HincII 2456 GTCAAC GT[CT]^[GA]AC
HindII 2456 GTCAAC GT[CT]^[GA]AC
HindIII 3676 AAGCTT A^AGCTT
MfeI 2979 CAATTG C^AATTG
MunI 2979 CAATTG C^AATTG
NciI 1862 CCGGG CC^[GC]GG
NdeI 3334 CATATG CA^TATG
NspV 3261 TTCGAA TT^CGAA
PmaCI 1746 CACGTG CAC^GTG
PmlI 1746 CACGTG CAC^GTG
PpuMI 594 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 845 ATTAAT AT^TAAT
RcaI 2746 TCATGA T^CATGA
SacI 1642 GAGCTC GAGCT^C
SapI 1909 GAAGAGC GCTCTTC(1/4)
ScaI 3491 AGTACT AGT^ACT
SfuI 3261 TTCGAA TT^CGAA
StuI 2356 AGGCCT AGG^CCT
StyI 1242 CCAAGG C^C[AT][AT]GG
XbaI 2618 TCTAGA T^CTAGA
XmnI 3295 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 260 GTCTAC GT^[AC][GT]AC
739 GTCTAC
AflIII 2705 ACATGT A^C[GA][CT]GT
2756 ACGTGT
BglII 1608 AGATCT A^GATCT
1995 AGATCT
BsaAI 1746 CACGTG [CT]AC^GT[GA]
2783 CACGTA
Bsp1407I 1617 TGTACA T^GTACA
3004 TGTACA
BsrGI 1617 TGTACA T^GTACA
3004 TGTACA
BssSI 1428 CTCGTG CACGAG(-5/-1)
2488 CTCGTG
BstEII 1234 GGTGACC G^GT[ACGT]ACC
1960 GGTAACC
BstPI 1234 GGTGACC G^GT[ACGT]ACC
1960 GGTAACC
CfoI 1185 GCGC GCG^C
2860 GCGC
DraII 594 GGGACCT [GA]G^G[ACGT]CC[CT]
2244 GGGCCCC
EarI 1909 GAAGAG CTCTTC(1/4)
2429 GAAGAG
EcoO109I 594 GGGACCT [GA]G^G[ACGT]CC[CT]
2244 GGGCCCC
EcoO65I 1234 GGTGACC G^GT[ACGT]ACC
1960 GGTAACC
EcoRI 469 GAATTC G^AATTC
2729 GAATTC
HaeII 1184 AGCGCC [GA]GCGC^[CT]
2859 GGCGCT
HhaI 1185 GCGC GCG^C
2860 GCGC
HinP1I 1185 GCGC G^CGC
2860 GCGC
Ksp632I 1909 GAAGAG CTCTTC(1/4)
2429 GAAGAG
PaeR7I 381 CTCGAG C^TCGAG
441 CTCGAG
PstI 1005 CTGCAG CTGCA^G
1119 CTGCAG
PvuII 33 CAGCTG CAG^CTG
637 CAGCTG
SspBI 1617 TGTACA T^GTACA
3004 TGTACA
XcmI 1716 CCAATTCCTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2515 CCATTATGCCGTTGG
XhoI 381 CTCGAG C^TCGAG
441 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII