##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
BamHI BbeI BbrPI BbvCI BcgI
BfrI BglII BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BstAPI BstBI
Bsu36I Cfr10I ClaI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FseI Hin1I HpaI
KasI KpnI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI SacI
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SwaI Tth111I Van91I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AclI 1316 AACGTT AA^CGTT
AflIII 2560 ACATGT A^C[GA][CT]GT
AlwNI 205 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 343 TGCGCA TGC^GCA
AvrII 2502 CCTAGG C^CTAGG
BalI 2326 TGGCCA TGG^CCA
BciVI 1125 GTATCC GTATCC(6/5)
BclI 2200 TGATCA T^GATCA
BglI 1282 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2502 CCTAGG C^CTAGG
BlpI 971 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 971 GCTTAGC GC^T[ACGT]AGC
BsaBI 2201 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 295 GAGACC GGTCTC(1/5)
Bsp1407I 1791 TGTACA T^GTACA
BspHI 723 TCATGA T^CATGA
BspLU11I 2560 ACATGT A^CATGT
BspMI 511 ACCTGC ACCTGC(4/8)
BsrDI 2417 GCAATG GCAATG(2/0)
BsrGI 1791 TGTACA T^GTACA
BssSI 1892 CTCGTG CACGAG(-5/-1)
Bst1107I 842 GTATAC GTA^TAC
BstEII 1949 GGTCACC G^GT[ACGT]ACC
BstPI 1949 GGTCACC G^GT[ACGT]ACC
BstXI 1129 CCACCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 842 GTATAC GTA^TAC
CelII 971 GCTTAGC GC^T[ACGT]AGC
CpoI 486 CGGACCG CG^G[AT]CCG
DraI 2393 TTTAAA TTT^AAA
DraII 899 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1872 GACAATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2326 TGGCCA [CT]^GGCC[GA]
EcoNI 512 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 899 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1949 GGTCACC G^GT[ACGT]ACC
FbaI 2200 TGATCA T^GATCA
FspI 343 TGCGCA TGC^GCA
HaeII 407 AGCGCC [GA]GCGC^[CT]
MamI 2201 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2326 TGGCCA TGG^CCA
MscI 2326 TGGCCA TGG^CCA
NheI 928 GCTAGC G^CTAGC
PpuMI 899 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1316 AACGTT AA^CGTT
PvuII 947 CAGCTG CAG^CTG
RcaI 723 TCATGA T^CATGA
RsrII 486 CGGACCG CG^G[AT]CCG
ScaI 179 AGTACT AGT^ACT
SexAI 517 ACCAGGT A^CC[AT]GGT
SmaI 424 CCCGGG CCC^GGG
SmlI 175 CTTGAG C^T[CT][GA]AG
SnaBI 1052 TACGTA TAC^GTA
SspBI 1791 TGTACA T^GTACA
StuI 1662 AGGCCT AGG^CCT
XmaCI 424 CCCGGG C^CCGGG
XmaI 424 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 842 GTATAC GT^[AC][GT]AC
1357 GTAGAC
AvaI 424 CCCGGG C^[CT]CG[GA]G
1944 CTCGGG
BanI 1399 GGTGCC G^G[CT][GA]CC
1901 GGCACC
BbsI 793 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BpuAI 793 GAAGAC GAAGAC(2/6)
1144 GAAGAC
BsaAI 1052 TACGTA [CT]AC^GT[GA]
2196 TACGTG
BsgI 43 CTGCAC GTGCAG(16/14)
513 CTGCAC
BsmI 1862 GAATGC GAATGC(1/-1)
565 GCATTC
BsoBI 424 CCCGGG C^[CT]CG[GA]G
1944 CTCGGG
BsrBI 14 CCGCTC CCGCTC(-3/-3)
390 GAGCGG
BstYI 1059 AGATCC [GA]^GATC[CT]
1307 GGATCT
HgaI 231 GCGTC GACGC(5/10)
439 GCGTC
HincII 1313 GTCAAC GT[CT]^[GA]AC
1524 GTCAAC
HindII 1313 GTCAAC GT[CT]^[GA]AC
1524 GTCAAC
HindIII 640 AAGCTT A^AGCTT
1685 AAGCTT
KspI 26 CCGCGG CCGC^GG
411 CCGCGG
MfeI 1547 CAATTG C^AATTG
2272 CAATTG
MflI 1059 AGATCC [GA]^GATC[CT]
1307 GGATCT
MunI 1547 CAATTG C^AATTG
2272 CAATTG
NcoI 1303 CCATGG C^CATGG
2010 CCATGG
NspI 1090 GCATGT [GA]CATG^[CT]
2560 ACATGT
SacII 26 CCGCGG CCGC^GG
411 CCGCGG
XbaI 855 TCTAGA T^CTAGA
2224 TCTAGA
XhoII 1059 AGATCC [GA]^GATC[CT]
1307 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI