##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BciVI BfrI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HpaI KpnI KspI
MamI MluI MroI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1653 GACGTC GACGT^C
AccII 1937 CGCG CG^CG
AcsI 3364 GAATTT [GA]^AATT[CT]
Aor51HI 2 AGCGCT AGC^GCT
ApoI 3364 GAATTT [GA]^AATT[CT]
Asp700I 1607 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 677 CTCGGG C^[CT]CG[GA]G
BbeI 3048 GGCGCC GGCGC^C
BbsI 2583 GTCTTC GAAGAC(2/6)
BcgI 2433 GCATAGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2385 TGATCA T^GATCA
BlpI 807 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 807 GCTCAGC GC^T[ACGT]AGC
BpuAI 2583 GTCTTC GAAGAC(2/6)
BsaAI 2619 TACGTG [CT]AC^GT[GA]
BsmI 2893 GCATTC GAATGC(1/-1)
BsoBI 677 CTCGGG C^[CT]CG[GA]G
BstUI 1937 CGCG CG^CG
BstXI 2322 CCAGTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 807 GCTCAGC GC^T[ACGT]AGC
DraII 1369 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 2 AGCGCT AGC^GCT
EcoNI 2353 CCTCCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1369 GGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 2385 TGATCA T^GATCA
KasI 3048 GGCGCC G^GCGCC
MfeI 3326 CAATTG C^AATTG
MunI 3326 CAATTG C^AATTG
MvnI 1937 CGCG CG^CG
NaeI 717 GCCGGC GCC^GGC
NarI 3048 GGCGCC GG^CGCC
NgoMIV 717 GCCGGC G^CCGGC
NheI 2902 GCTAGC G^CTAGC
PpuMI 1369 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1669 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1919 AGTACT AGT^ACT
SexAI 1558 ACCAGGT A^CC[AT]GGT
SfoI 3048 GGCGCC GGC^GCC
StuI 2796 AGGCCT AGG^CCT
XbaI 732 TCTAGA T^CTAGA
XcmI 1300 CCAGAGTTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1607 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2008 GTAGAC GT^[AC][GT]AC
2833 GTCTAC
AcyI 1653 GACGTC G[GA]^CG[CT]C
3048 GGCGCC
BalI 878 TGGCCA TGG^CCA
2109 TGGCCA
BbvCI 705 CCTCAGC CCTCAGC(-5/-2)
2372 CCTCAGC
BcnI 364 CCCGG CC^[GC]GG
518 CCCGG
BglII 1929 AGATCT A^GATCT
3227 AGATCT
BsaHI 1653 GACGTC G[GA]^CG[CT]C
3048 GGCGCC
BsgI 136 CTGCAC GTGCAG(16/14)
1660 GTGCAG
Bsp1407I 142 TGTACA T^GTACA
1413 TGTACA
BspMI 700 ACCTGC ACCTGC(4/8)
1662 GCAGGT
BsrDI 1375 CATTGC GCAATG(2/0)
2408 CATTGC
BsrFI 433 ACCGGC [GA]^CCGG[CT]
717 GCCGGC
BsrGI 142 TGTACA T^GTACA
1413 TGTACA
BssSI 61 CACGAG CACGAG(-5/-1)
1176 CACGAG
BstAPI 922 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1220 GCAACCACTGC
CfoI 3 GCGC GCG^C
3049 GCGC
Cfr10I 433 ACCGGC [GA]^CCGG[CT]
717 GCCGGC
HaeII 2 AGCGCT [GA]GCGC^[CT]
3048 GGCGCC
HgaI 1876 GCGTC GACGC(5/10)
1938 GCGTC
HhaI 3 GCGC GCG^C
3049 GCGC
Hin1I 1653 GACGTC G[GA]^CG[CT]C
3048 GGCGCC
HinP1I 3 GCGC G^CGC
3049 GCGC
MluNI 878 TGGCCA TGG^CCA
2109 TGGCCA
MscI 878 TGGCCA TGG^CCA
2109 TGGCCA
NciI 364 CCCGG CC^[GC]GG
518 CCCGG
NcoI 1309 CCATGG C^CATGG
2263 CCATGG
NdeI 1743 CATATG CA^TATG
2065 CATATG
NspI 481 ACATGC [GA]CATG^[CT]
1006 GCATGT
PflMI 60 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2944 CCATCAAATGG
PstI 6 CTGCAG CTGCA^G
929 CTGCAG
SapI 648 GAAGAGC GCTCTTC(1/4)
725 GCTCTTC
SspBI 142 TGTACA T^GTACA
1413 TGTACA
TfiI 1722 GAATC G^A[AT]TC
2078 GATTC
Van91I 60 CCACGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2944 CCATCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BanI BanII BbvI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII