##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
AscI Asp718I AspEI AviII BanI
BbeI BbvCI BclI BfrI BsaBI
BsaHI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp45I Van91I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1613 TCCGGA T^CCGGA
Alw44I 3138 GTGCAC G^TGCAC
ApaLI 3138 GTGCAC G^TGCAC
AspI 3125 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1974 CCTAGG C^CTAGG
BbrPI 2141 CACGTG CAC^GTG
BcgI 2278 GCAGGTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 8 GTATCC GTATCC(6/5)
BglII 379 AGATCT A^GATCT
BlnI 1974 CCTAGG C^CTAGG
BsaAI 2141 CACGTG [CT]AC^GT[GA]
BsaI 1876 GGTCTC GGTCTC(1/5)
BseAI 1613 TCCGGA T^CCGGA
BspEI 1613 TCCGGA T^CCGGA
BssHII 709 GCGCGC G^CGCGC
BstXI 1064 CCAGGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2381 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 1933 GAATTC G^AATTC
MaeII 2142 ACGT A^CGT
MaeIII 179 GTAAC ^GT[ACGT]AC
MroI 1613 TCCGGA T^CCGGA
NspI 774 GCATGC [GA]CATG^[CT]
PflFI 3125 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2141 CACGTG CAC^GTG
PmlI 2141 CACGTG CAC^GTG
PshAI 913 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 173 AGTACT AGT^ACT
SfiI 2629 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 774 GCATGC GCATG^C
StuI 989 AGGCCT AGG^CCT
Tth111I 3125 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 2108 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3123 CAGACACTG
AseI 215 ATTAAT AT^TAAT
470 ATTAAT
AsnI 215 ATTAAT AT^TAAT
470 ATTAAT
BamHI 131 GGATCC G^GATCC
1815 GGATCC
BsaWI 1613 TCCGGA [AT]^CCGG[AT]
1919 TCCGGT
BsmBI 1204 GAGACG CGTCTC(1/5)
2844 GAGACG
BsmI 155 GAATGC GAATGC(1/-1)
2878 GAATGC
BsrDI 272 GCAATG GCAATG(2/0)
2857 GCAATG
BssSI 1420 CACGAG CACGAG(-5/-1)
2891 CTCGTG
BstAPI 1419 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1895 GCAAACAGTGC
EagI 720 CGGCCG C^GGCCG
873 CGGCCG
EclXI 720 CGGCCG C^GGCCG
873 CGGCCG
Eco52I 720 CGGCCG C^GGCCG
873 CGGCCG
EcoNI 2113 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2362 CCTCATTGAGG
HgaI 2037 GCGTC GACGC(5/10)
2566 GCGTC
NaeI 1404 GCCGGC GCC^GGC
2607 GCCGGC
NgoMIV 1404 GCCGGC G^CCGGC
2607 GCCGGC
PshBI 215 ATTAAT AT^TAAT
470 ATTAAT
SacI 1372 GAGCTC GAGCT^C
2510 GAGCTC
TfiI 2221 GATTC G^A[AT]TC
3227 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI ApaI ApoI Asp700I
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI
XmnI