##################################
# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BclI BcnI BfrI BglI
BlnI BpmI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BsoBI BspDI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MluI MroI MspI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AciI 887 GCGG CCGC(-3/-1)
AocI 1224 CCTGAGG CC^T[ACGT]AGG
AspHI 781 GAGCAC G[AT]GC[AT]^C
AvaII 1878 GGTCC G^G[AT]CC
BalI 2254 TGGCCA TGG^CCA
BbvCI 327 GCTGAGG CCTCAGC(-5/-2)
BlpI 63 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC
BsaBI 153 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 781 GAGCAC G[AT]GC[AT]^C
BsmBI 680 GAGACG CGTCTC(1/5)
BspHI 2366 TCATGA T^CATGA
BstAPI 2794 GCATGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 986 CCAGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1224 CCTGAGG CC^T[ACGT]AGG
CelII 63 GCTGAGC GC^T[ACGT]AGC
DsaI 1478 CCATGG C^C[GA][CT]GG
EaeI 2254 TGGCCA [CT]^GGCC[GA]
Eco81I 1224 CCTGAGG CC^T[ACGT]AGG
FauI 887 GCGGG CCCGC(4/6)
HphI 1671 GGTGA GGTGA(8/7)
MamI 153 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2323 CAATTG C^AATTG
MluNI 2254 TGGCCA TGG^CCA
MscI 2254 TGGCCA TGG^CCA
MunI 2323 CAATTG C^AATTG
NcoI 1478 CCATGG C^CATGG
NlaIV 2750 GGAACC GG[ACGT]^[ACGT]CC
RcaI 2366 TCATGA T^CATGA
SapI 1492 GAAGAGC GCTCTTC(1/4)
SspI 2344 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AclI 469 AACGTT AA^CGTT
2233 AACGTT
BanII 96 GAGCCC G[GA]GC[CT]^C
1181 GGGCTC
BciVI 3086 GGATAC GTATCC(6/5)
1955 GTATCC
BseRI 3263 CTCCTC GAGGAG(10/8)
2095 GAGGAG
BsgI 2479 CTGCAC GTGCAG(16/14)
3134 GTGCAG
BsiYI 1508 CCTGGAACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2542 CCTGTTTATGG
BslI 1508 CCTGGAACGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
2542 CCTGTTTATGG
Bsp1407I 2141 TGTACA T^GTACA
3462 TGTACA
BsrGI 2141 TGTACA T^GTACA
3462 TGTACA
EcoT14I 1478 CCATGG C^C[AT][AT]GG
1811 CCAAGG
HincII 372 GTTGAC GT[CT]^[GA]AC
2881 GTTGAC
HindII 372 GTTGAC GT[CT]^[GA]AC
2881 GTTGAC
HindIII 1444 AAGCTT A^AGCTT
3362 AAGCTT
MspA1I 325 CAGCTG C[AC]G^C[GT]G
3583 CAGCTG
Psp1406I 469 AACGTT AA^CGTT
2233 AACGTT
PstI 525 CTGCAG CTGCA^G
3037 CTGCAG
PvuII 325 CAGCTG CAG^CTG
3583 CAGCTG
SspBI 2141 TGTACA T^GTACA
3462 TGTACA
StyI 1478 CCATGG C^C[AT][AT]GG
1811 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwNI
ApoI AseI AsnI Asp700I BbsI
BbvI BfaI BglII BmyI BpuAI
BsaJI BsmAI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoRII EcoT22I Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI MwoI NdeI
NdeII NlaIII NsiI NspI PleI
PshBI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI