##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BciVI BlnI BsaBI BsaHI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I ClaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
Hin1I KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmeI PshBI Psp1406I
PstI PvuI RcaI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 674 GTAGAC GT^[AC][GT]AC
AflII 374 CTTAAG C^TTAAG
ApaI 1822 GGGCCC GGGCC^C
AspI 515 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1854 GGATCC G^GATCC
BbrPI 3025 CACGTG CAC^GTG
BfrI 374 CTTAAG C^TTAAG
BglII 1698 AGATCT A^GATCT
BlpI 1459 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1459 GCTGAGC GC^T[ACGT]AGC
BsaAI 3025 CACGTG [CT]AC^GT[GA]
BsiEI 667 CGACCG CG[GA][CT]^CG
Bsp1407I 1503 TGTACA T^GTACA
BspMI 508 GCAGGT ACCTGC(4/8)
BsrFI 1776 GCCGGT [GA]^CCGG[CT]
BsrGI 1503 TGTACA T^GTACA
BstAPI 2732 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2484 GGTCACC G^GT[ACGT]ACC
BstPI 2484 GGTCACC G^GT[ACGT]ACC
CelII 1459 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1776 GCCGGT [GA]^CCGG[CT]
DraIII 191 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2901 GACACCGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2484 GGTCACC G^GT[ACGT]ACC
EcoT22I 2072 ATGCAT ATGCA^T
HaeII 794 AGCGCC [GA]GCGC^[CT]
HpaI 2312 GTTAAC GTT^AAC
KspI 2562 CCGCGG CCGC^GG
MaeII 3026 ACGT A^CGT
NcoI 412 CCATGG C^CATGG
NdeI 2650 CATATG CA^TATG
NsiI 2072 ATGCAT ATGCA^T
NspI 610 GCATGC [GA]CATG^[CT]
PaeR7I 1417 CTCGAG C^TCGAG
PflFI 515 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3025 CACGTG CAC^GTG
PmlI 3025 CACGTG CAC^GTG
PshAI 3043 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2132 GAGCTC GAGCT^C
SacII 2562 CCGCGG CCGC^GG
SapI 1786 GCTCTTC GCTCTTC(1/4)
SphI 610 GCATGC GCATG^C
SspBI 1503 TGTACA T^GTACA
Tth111I 515 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2267 TCTAGA T^CTAGA
XhoI 1417 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AspHI 2132 GAGCTC G[AT]GC[AT]^C
2336 GAGCAC
BbsI 598 GAAGAC GAAGAC(2/6)
1174 GAAGAC
BbvCI 1391 CCTCAGC CCTCAGC(-5/-2)
340 GCTGAGG
BcgI 1105 GCAGAAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3124 GCAATGAGGTCG
BclI 565 TGATCA T^GATCA
2827 TGATCA
BglI 1542 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1562 GCCACAGAGGC
BpuAI 598 GAAGAC GAAGAC(2/6)
1174 GAAGAC
BsiHKAI 2132 GAGCTC G[AT]GC[AT]^C
2336 GAGCAC
BsmI 1126 GAATGC GAATGC(1/-1)
2138 GCATTC
BsrBI 15 GAGCGG CCGCTC(-3/-3)
1307 GAGCGG
BsrDI 1961 CATTGC GCAATG(2/0)
3124 GCAATG
BstXI 412 CCATGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1797 CCAACTATGTGG
BstYI 1698 AGATCT [GA]^GATC[CT]
1854 GGATCC
CpoI 53 CGGACCG CG^G[AT]CCG
519 CGGTCCG
DraI 121 TTTAAA TTT^AAA
3196 TTTAAA
EarI 1787 CTCTTC CTCTTC(1/4)
1322 GAAGAG
EcoRI 3162 GAATTC G^AATTC
3207 GAATTC
FbaI 565 TGATCA T^GATCA
2827 TGATCA
HincII 1132 GTCAAC GT[CT]^[GA]AC
2312 GTTAAC
HindII 1132 GTCAAC GT[CT]^[GA]AC
2312 GTTAAC
HindIII 177 AAGCTT A^AGCTT
405 AAGCTT
Ksp632I 1787 CTCTTC CTCTTC(1/4)
1322 GAAGAG
MflI 1698 AGATCT [GA]^GATC[CT]
1854 GGATCC
PflMI 86 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1291 CCAGCGGATGG
PvuII 462 CAGCTG CAG^CTG
938 CAGCTG
RsrII 53 CGGACCG CG^G[AT]CCG
519 CGGTCCG
SmaI 46 CCCGGG CCC^GGG
1594 CCCGGG
SmlI 374 CTTAAG C^T[CT][GA]AG
1417 CTCGAG
StuI 1874 AGGCCT AGG^CCT
2206 AGGCCT
Van91I 86 CCAATGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1291 CCAGCGGATGG
XcmI 1329 CCAGGAGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2649 CCATATGGTCCCTGG
XhoII 1698 AGATCT [GA]^GATC[CT]
1854 GGATCC
XmaCI 46 CCCGGG C^CCGGG
1594 CCCGGG
XmaI 46 CCCGGG C^CCGGG
1594 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BssSI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I