##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BbeI        
BciVI       BlnI        BsaBI       BsaHI       BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
Hin1I       KasI        KpnI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PinAI       PmeI        PshBI       Psp1406I    
PstI        PvuI        RcaI        SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspI        
SwaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          674  GTAGAC               GT^[AC][GT]AC
AflII         374  CTTAAG               C^TTAAG
ApaI         1822  GGGCCC               GGGCC^C
AspI          515  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1854  GGATCC               G^GATCC
BbrPI        3025  CACGTG               CAC^GTG
BfrI          374  CTTAAG               C^TTAAG
BglII        1698  AGATCT               A^GATCT
BlpI         1459  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1459  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3025  CACGTG               [CT]AC^GT[GA]
BsiEI         667  CGACCG               CG[GA][CT]^CG
Bsp1407I     1503  TGTACA               T^GTACA
BspMI         508  GCAGGT               ACCTGC(4/8)
BsrFI        1776  GCCGGT               [GA]^CCGG[CT]
BsrGI        1503  TGTACA               T^GTACA
BstAPI       2732  GCAGTGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       2484  GGTCACC              G^GT[ACGT]ACC
BstPI        2484  GGTCACC              G^GT[ACGT]ACC
CelII        1459  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1776  GCCGGT               [GA]^CCGG[CT]
DraIII        191  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2901  GACACCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      2484  GGTCACC              G^GT[ACGT]ACC
EcoT22I      2072  ATGCAT               ATGCA^T
HaeII         794  AGCGCC               [GA]GCGC^[CT]
HpaI         2312  GTTAAC               GTT^AAC
KspI         2562  CCGCGG               CCGC^GG
MaeII        3026  ACGT                 A^CGT
NcoI          412  CCATGG               C^CATGG
NdeI         2650  CATATG               CA^TATG
NsiI         2072  ATGCAT               ATGCA^T
NspI          610  GCATGC               [GA]CATG^[CT]
PaeR7I       1417  CTCGAG               C^TCGAG
PflFI         515  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        3025  CACGTG               CAC^GTG
PmlI         3025  CACGTG               CAC^GTG
PshAI        3043  GACTTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         2132  GAGCTC               GAGCT^C
SacII        2562  CCGCGG               CCGC^GG
SapI         1786  GCTCTTC              GCTCTTC(1/4)
SphI          610  GCATGC               GCATG^C
SspBI        1503  TGTACA               T^GTACA
Tth111I       515  GACTCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         2267  TCTAGA               T^CTAGA
XhoI         1417  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AspHI        2132  GAGCTC               G[AT]GC[AT]^C
             2336  GAGCAC              
BbsI          598  GAAGAC               GAAGAC(2/6)
             1174  GAAGAC              
BbvCI        1391  CCTCAGC              CCTCAGC(-5/-2)
              340  GCTGAGG             
BcgI         1105  GCAGAAGGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3124  GCAATGAGGTCG        
BclI          565  TGATCA               T^GATCA
             2827  TGATCA              
BglI         1542  GCCAGCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1562  GCCACAGAGGC         
BpuAI         598  GAAGAC               GAAGAC(2/6)
             1174  GAAGAC              
BsiHKAI      2132  GAGCTC               G[AT]GC[AT]^C
             2336  GAGCAC              
BsmI         1126  GAATGC               GAATGC(1/-1)
             2138  GCATTC              
BsrBI          15  GAGCGG               CCGCTC(-3/-3)
             1307  GAGCGG              
BsrDI        1961  CATTGC               GCAATG(2/0)
             3124  GCAATG              
BstXI         412  CCATGGCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1797  CCAACTATGTGG        
BstYI        1698  AGATCT               [GA]^GATC[CT]
             1854  GGATCC              
CpoI           53  CGGACCG              CG^G[AT]CCG
              519  CGGTCCG             
DraI          121  TTTAAA               TTT^AAA
             3196  TTTAAA              
EarI         1787  CTCTTC               CTCTTC(1/4)
             1322  GAAGAG              
EcoRI        3162  GAATTC               G^AATTC
             3207  GAATTC              
FbaI          565  TGATCA               T^GATCA
             2827  TGATCA              
HincII       1132  GTCAAC               GT[CT]^[GA]AC
             2312  GTTAAC              
HindII       1132  GTCAAC               GT[CT]^[GA]AC
             2312  GTTAAC              
HindIII       177  AAGCTT               A^AGCTT
              405  AAGCTT              
Ksp632I      1787  CTCTTC               CTCTTC(1/4)
             1322  GAAGAG              
MflI         1698  AGATCT               [GA]^GATC[CT]
             1854  GGATCC              
PflMI          86  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1291  CCAGCGGATGG         
PvuII         462  CAGCTG               CAG^CTG
              938  CAGCTG              
RsrII          53  CGGACCG              CG^G[AT]CCG
              519  CGGTCCG             
SmaI           46  CCCGGG               CCC^GGG
             1594  CCCGGG              
SmlI          374  CTTAAG               C^T[CT][GA]AG
             1417  CTCGAG              
StuI         1874  AGGCCT               AGG^CCT
             2206  AGGCCT              
Van91I         86  CCAATGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1291  CCAGCGGATGG         
XcmI         1329  CCAGGAGGAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2649  CCATATGGTCCCTGG     
XhoII        1698  AGATCT               [GA]^GATC[CT]
             1854  GGATCC              
XmaCI          46  CCCGGG               C^CCGGG
             1594  CCCGGG              
XmaI           46  CCCGGG               C^CCGGG
             1594  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        AvaI        
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BssSI       BstF5I      BstNI       BstUI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I