##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AscI AseI AsnI Asp700I Asp718I
AvrII BciVI BclI BfrI BlnI
BsaBI BsmI Bsp1407I BspDI BspHI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EcoRI EcoRV EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2248 GACGTC GACGT^C
AcsI 3242 GAATTT [GA]^AATT[CT]
AhdI 676 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1201 GTGCAC G^TGCAC
Aor51HI 1849 AGCGCT AGC^GCT
ApaLI 1201 GTGCAC G^TGCAC
ApoI 3242 GAATTT [GA]^AATT[CT]
AspEI 676 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1609 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2281 CACGTG CAC^GTG
BglII 1859 AGATCT A^GATCT
BsaAI 2281 CACGTG [CT]AC^GT[GA]
BsiWI 1709 CGTACG C^GTACG
BspLU11I 2793 ACATGT A^CATGT
BstEII 1668 GGTCACC G^GT[ACGT]ACC
BstPI 1668 GGTCACC G^GT[ACGT]ACC
EagI 257 CGGCCG C^GGCCG
Eam1105I 676 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 257 CGGCCG C^GGCCG
Eco47III 1849 AGCGCT AGC^GCT
Eco52I 257 CGGCCG C^GGCCG
EcoO65I 1668 GGTCACC G^GT[ACGT]ACC
HincII 1372 GTCAAC GT[CT]^[GA]AC
HindII 1372 GTCAAC GT[CT]^[GA]AC
HindIII 3282 AAGCTT A^AGCTT
MslI 1467 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 1609 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2281 CACGTG CAC^GTG
PmlI 2281 CACGTG CAC^GTG
SexAI 2144 ACCTGGT A^CC[AT]GGT
SphI 719 GCATGC GCATG^C
Tth111I 1609 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2493 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1223 TCCGGA T^CCGGA
2585 TCCGGA
AflIII 1643 ACGTGT A^C[GA][CT]GT
2793 ACATGT
AviII 611 TGCGCA TGC^GCA
1397 TGCGCA
BalI 2016 TGGCCA TGG^CCA
2988 TGGCCA
BamHI 72 GGATCC G^GATCC
2578 GGATCC
BbeI 836 GGCGCC GGCGC^C
1723 GGCGCC
BbsI 753 GAAGAC GAAGAC(2/6)
1615 GTCTTC
BcgI 108 CGAGGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
855 CGAGACCATTGC
BlpI 118 GCTGAGC GC^T[ACGT]AGC
3284 GCTTAGC
Bpu1102I 118 GCTGAGC GC^T[ACGT]AGC
3284 GCTTAGC
BpuAI 753 GAAGAC GAAGAC(2/6)
1615 GTCTTC
BsaI 856 GAGACC GGTCTC(1/5)
3234 GGTCTC
BsaWI 1223 TCCGGA [AT]^CCGG[AT]
2585 TCCGGA
BseAI 1223 TCCGGA T^CCGGA
2585 TCCGGA
BsmBI 2771 CGTCTC CGTCTC(1/5)
2392 GAGACG
BspEI 1223 TCCGGA T^CCGGA
2585 TCCGGA
BsrDI 861 CATTGC GCAATG(2/0)
2253 CATTGC
CelII 118 GCTGAGC GC^T[ACGT]AGC
3284 GCTTAGC
FspI 611 TGCGCA TGC^GCA
1397 TGCGCA
KasI 836 GGCGCC G^GCGCC
1723 GGCGCC
MluNI 2016 TGGCCA TGG^CCA
2988 TGGCCA
MroI 1223 TCCGGA T^CCGGA
2585 TCCGGA
MscI 2016 TGGCCA TGG^CCA
2988 TGGCCA
MseI 3268 TTAA T^TAA
3303 TTAA
NarI 836 GGCGCC GG^CGCC
1723 GGCGCC
NspI 719 GCATGC [GA]CATG^[CT]
2793 ACATGT
PflMI 126 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3188 CCAGCGGGTGG
SacI 1035 GAGCTC GAGCT^C
1444 GAGCTC
SbfI 441 CCTGCAGG CCTGCA^GG
975 CCTGCAGG
SfoI 836 GGCGCC GGC^GCC
1723 GGCGCC
SmaI 50 CCCGGG CCC^GGG
285 CCCGGG
Sse8387I 441 CCTGCAGG CCTGCA^GG
975 CCTGCAGG
Tru9I 3268 TTAA T^TAA
3303 TTAA
Tsp509I 2840 AATT ^AATT
3243 AATT
Van91I 126 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3188 CCAGCGGGTGG
XcmI 1175 CCAGAGCCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2891 CCATCAGCGAACTGG
XmaCI 50 CCCGGG C^CCGGG
285 CCCGGG
XmaI 50 CCCGGG C^CCGGG
285 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII