##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AclI AflII AhdI AocI AscI
AseI AsnI AspEI AspI AviII
AvrII BalI BbvCI BciVI BfrI
BlnI BlpI Bpu1102I BsaBI BsiWI
BspDI BsrBI BssHII BstAPI BstBI
BstEII BstPI Bsu36I CelII ClaI
CpoI Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI KspI MamI
MluI MluNI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 3430 GACGTC GACGT^C
Acc65I 3595 GGTACC G^GTACC
AccIII 977 TCCGGA T^CCGGA
AgeI 2563 ACCGGT A^CCGGT
ApaI 797 GGGCCC GGGCC^C
Asp700I 2433 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3595 GGTACC G^GTACC
BbeI 2895 GGCGCC GGCGC^C
BbrPI 3355 CACGTG CAC^GTG
BclI 2410 TGATCA T^GATCA
BsaAI 3355 CACGTG [CT]AC^GT[GA]
BseAI 977 TCCGGA T^CCGGA
BsiEI 2039 CGGCCG CG[GA][CT]^CG
BsmI 2589 GAATGC GAATGC(1/-1)
Bsp1407I 3203 TGTACA T^GTACA
BspEI 977 TCCGGA T^CCGGA
BspHI 485 TCATGA T^CATGA
BsrDI 769 GCAATG GCAATG(2/0)
BsrGI 3203 TGTACA T^GTACA
Bst1107I 698 GTATAC GTA^TAC
BstZ17I 698 GTATAC GTA^TAC
DraI 59 TTTAAA TTT^AAA
DraIII 2076 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2862 GACAACGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2039 CGGCCG C^GGCCG
EclXI 2039 CGGCCG C^GGCCG
Eco52I 2039 CGGCCG C^GGCCG
EcoNI 3410 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 519 GAATTC G^AATTC
FbaI 2410 TGATCA T^GATCA
HincII 3234 GTTAAC GT[CT]^[GA]AC
HindII 3234 GTTAAC GT[CT]^[GA]AC
HindIII 777 AAGCTT A^AGCTT
HpaI 3234 GTTAAC GTT^AAC
KasI 2895 GGCGCC G^GCGCC
KpnI 3595 GGTACC GGTAC^C
MfeI 346 CAATTG C^AATTG
MroI 977 TCCGGA T^CCGGA
MunI 346 CAATTG C^AATTG
NarI 2895 GGCGCC GG^CGCC
PinAI 2563 ACCGGT A^CCGGT
PmaCI 3355 CACGTG CAC^GTG
PmlI 3355 CACGTG CAC^GTG
PvuII 813 CAGCTG CAG^CTG
RcaI 485 TCATGA T^CATGA
SacI 1035 GAGCTC GAGCT^C
ScaI 1195 AGTACT AGT^ACT
SexAI 1648 ACCTGGT A^CC[AT]GGT
SfiI 798 GGCCCTAAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2895 GGCGCC GGC^GCC
SspBI 3203 TGTACA T^GTACA
StuI 1336 AGGCCT AGG^CCT
XcmI 1147 CCACCCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2433 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 264 AGCGCT AGC^GCT
1674 AGCGCT
BcgI 985 GCACGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1228 GCACCAAGGTCG
BglI 799 GCCCTAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2845 GCCTCCATGGC
BglII 458 AGATCT A^GATCT
1552 AGATCT
BsaWI 977 TCCGGA [AT]^CCGG[AT]
2563 ACCGGT
BspLU11I 854 ACATGT A^CATGT
1796 ACATGT
BssSI 986 CACGAG CACGAG(-5/-1)
1320 CACGAG
BstXI 871 CCATCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1606 CCAACACGGTGG
Eco47III 264 AGCGCT AGC^GCT
1674 AGCGCT
PflMI 1151 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1688 CCATAGCATGG
PpuMI 1573 AGGACCT [GA]G^G[AT]CC[CT]
1721 AGGTCCT
SmaI 1949 CCCGGG CCC^GGG
2826 CCCGGG
SmlI 1710 CTCAAG C^T[CT][GA]AG
3414 CTCAAG
TaqI 1978 TCGA T^CGA
2378 TCGA
TthHB8I 1978 TCGA T^CGA
2378 TCGA
Van91I 1151 CCATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1688 CCATAGCATGG
XmaCI 1949 CCCGGG C^CCGGG
2826 CCCGGG
XmaI 1949 CCCGGG C^CCGGG
2826 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII