##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AclI        AflII       AhdI        AocI        AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BalI        BbvCI       BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsiWI       
BspDI       BsrBI       BssHII      BstAPI      BstBI       
BstEII      BstPI       Bsu36I      CelII       ClaI        
CpoI        Eam1105I    Eco81I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        KspI        MamI        
MluI        MluNI       MscI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AatII        3430  GACGTC               GACGT^C
Acc65I       3595  GGTACC               G^GTACC
AccIII        977  TCCGGA               T^CCGGA
AgeI         2563  ACCGGT               A^CCGGT
ApaI          797  GGGCCC               GGGCC^C
Asp700I      2433  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3595  GGTACC               G^GTACC
BbeI         2895  GGCGCC               GGCGC^C
BbrPI        3355  CACGTG               CAC^GTG
BclI         2410  TGATCA               T^GATCA
BsaAI        3355  CACGTG               [CT]AC^GT[GA]
BseAI         977  TCCGGA               T^CCGGA
BsiEI        2039  CGGCCG               CG[GA][CT]^CG
BsmI         2589  GAATGC               GAATGC(1/-1)
Bsp1407I     3203  TGTACA               T^GTACA
BspEI         977  TCCGGA               T^CCGGA
BspHI         485  TCATGA               T^CATGA
BsrDI         769  GCAATG               GCAATG(2/0)
BsrGI        3203  TGTACA               T^GTACA
Bst1107I      698  GTATAC               GTA^TAC
BstZ17I       698  GTATAC               GTA^TAC
DraI           59  TTTAAA               TTT^AAA
DraIII       2076  CACGAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2862  GACAACGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2039  CGGCCG               C^GGCCG
EclXI        2039  CGGCCG               C^GGCCG
Eco52I       2039  CGGCCG               C^GGCCG
EcoNI        3410  CCTCCTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         519  GAATTC               G^AATTC
FbaI         2410  TGATCA               T^GATCA
HincII       3234  GTTAAC               GT[CT]^[GA]AC
HindII       3234  GTTAAC               GT[CT]^[GA]AC
HindIII       777  AAGCTT               A^AGCTT
HpaI         3234  GTTAAC               GTT^AAC
KasI         2895  GGCGCC               G^GCGCC
KpnI         3595  GGTACC               GGTAC^C
MfeI          346  CAATTG               C^AATTG
MroI          977  TCCGGA               T^CCGGA
MunI          346  CAATTG               C^AATTG
NarI         2895  GGCGCC               GG^CGCC
PinAI        2563  ACCGGT               A^CCGGT
PmaCI        3355  CACGTG               CAC^GTG
PmlI         3355  CACGTG               CAC^GTG
PvuII         813  CAGCTG               CAG^CTG
RcaI          485  TCATGA               T^CATGA
SacI         1035  GAGCTC               GAGCT^C
ScaI         1195  AGTACT               AGT^ACT
SexAI        1648  ACCTGGT              A^CC[AT]GGT
SfiI          798  GGCCCTAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         2895  GGCGCC               GGC^GCC
SspBI        3203  TGTACA               T^GTACA
StuI         1336  AGGCCT               AGG^CCT
XcmI         1147  CCACCCATGATCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2433  GAAAAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Aor51HI       264  AGCGCT               AGC^GCT
             1674  AGCGCT              
BcgI          985  GCACGAGACTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1228  GCACCAAGGTCG        
BglI          799  GCCCTAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2845  GCCTCCATGGC         
BglII         458  AGATCT               A^GATCT
             1552  AGATCT              
BsaWI         977  TCCGGA               [AT]^CCGG[AT]
             2563  ACCGGT              
BspLU11I      854  ACATGT               A^CATGT
             1796  ACATGT              
BssSI         986  CACGAG               CACGAG(-5/-1)
             1320  CACGAG              
BstXI         871  CCATCCGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1606  CCAACACGGTGG        
Eco47III      264  AGCGCT               AGC^GCT
             1674  AGCGCT              
PflMI        1151  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1688  CCATAGCATGG         
PpuMI        1573  AGGACCT              [GA]G^G[AT]CC[CT]
             1721  AGGTCCT             
SmaI         1949  CCCGGG               CCC^GGG
             2826  CCCGGG              
SmlI         1710  CTCAAG               C^T[CT][GA]AG
             3414  CTCAAG              
TaqI         1978  TCGA                 T^CGA
             2378  TCGA                
TthHB8I      1978  TCGA                 T^CGA
             2378  TCGA                
Van91I       1151  CCATGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1688  CCATAGCATGG         
XmaCI        1949  CCCGGG               C^CCGGG
             2826  CCCGGG              
XmaI         1949  CCCGGG               C^CCGGG
             2826  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        Alw44I      AlwI        
AlwNI       ApaLI       ApoI        AspHI       AvaI        
AvaII       BamHI       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII