##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI BbeI BbvCI
BclI BfrI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BsrFI
BssHII Bst1107I BstZ17I Cfr10I ClaI
CpoI DraIII EagI EclXI Eco52I
EcoRI EcoRV FbaI FseI HgaI
Hin1I HindIII HpaI KasI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SapI SbfI SfiI SfoI
SgrAI SmlI SpeI Sse8387I SspI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 2223 GGTACC G^GTACC
AccI 1568 GTCGAC GT^[AC][GT]AC
AccII 66 CGCG CG^CG
AcsI 310 AAATTC [GA]^AATT[CT]
AhdI 1111 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1660 CCTCAGG CC^T[ACGT]AGG
Aor51HI 914 AGCGCT AGC^GCT
ApoI 310 AAATTC [GA]^AATT[CT]
Asp718I 2223 GGTACC G^GTACC
AspEI 1111 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2127 GAGCAC G[AT]GC[AT]^C
AviII 1835 TGCGCA TGC^GCA
BalI 1002 TGGCCA TGG^CCA
BbrPI 654 CACGTG CAC^GTG
BcgI 2016 GCATTTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 549 GTATCC GTATCC(6/5)
BglII 1394 AGATCT A^GATCT
BlpI 2722 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2722 GCTAAGC GC^T[ACGT]AGC
BsaBI 868 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2127 GAGCAC G[AT]GC[AT]^C
BsmI 3711 GAATGC GAATGC(1/-1)
BssSI 1898 CTCGTG CACGAG(-5/-1)
BstAPI 1885 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2252 TTCGAA TT^CGAA
BstEII 3444 GGTCACC G^GT[ACGT]ACC
BstPI 3444 GGTCACC G^GT[ACGT]ACC
BstUI 66 CGCG CG^CG
BstXI 2228 CCACGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1660 CCTCAGG CC^T[ACGT]AGG
CelII 2722 GCTAAGC GC^T[ACGT]AGC
DraI 3669 TTTAAA TTT^AAA
DrdI 2551 GACCCAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1111 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 914 AGCGCT AGC^GCT
Eco81I 1660 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3444 GGTCACC G^GT[ACGT]ACC
EcoT22I 3634 ATGCAT ATGCA^T
FspI 1835 TGCGCA TGC^GCA
HaeII 914 AGCGCT [GA]GCGC^[CT]
KpnI 2223 GGTACC GGTAC^C
KspI 65 CCGCGG CCGC^GG
MamI 868 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1002 TGGCCA TGG^CCA
MscI 1002 TGGCCA TGG^CCA
MvnI 66 CGCG CG^CG
NsiI 3634 ATGCAT ATGCA^T
NspV 2252 TTCGAA TT^CGAA
PmaCI 654 CACGTG CAC^GTG
PmlI 654 CACGTG CAC^GTG
SacII 65 CCGCGG CCGC^GG
SalI 1568 GTCGAC G^TCGAC
ScaI 53 AGTACT AGT^ACT
SfuI 2252 TTCGAA TT^CGAA
SmaI 558 CCCGGG CCC^GGG
SnaBI 3578 TACGTA TAC^GTA
SphI 3632 GCATGC GCATG^C
StuI 2063 AGGCCT AGG^CCT
XbaI 567 TCTAGA T^CTAGA
XmaCI 558 CCCGGG C^CCGGG
XmaI 558 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
ApaI 3107 GGGCCC GGGCC^C
3126 GGGCCC
AvrII 715 CCTAGG C^CTAGG
2070 CCTAGG
BamHI 659 GGATCC G^GATCC
737 GGATCC
BlnI 715 CCTAGG C^CTAGG
2070 CCTAGG
BsaAI 654 CACGTG [CT]AC^GT[GA]
3578 TACGTA
BsaI 2549 GAGACC GGTCTC(1/5)
2782 GAGACC
BsgI 622 CTGCAC GTGCAG(16/14)
409 GTGCAG
BsmBI 1180 GAGACG CGTCTC(1/5)
1732 GAGACG
BspHI 596 TCATGA T^CATGA
1505 TCATGA
BspLU11I 213 ACATGT A^CATGT
1844 ACATGT
BspMI 1247 ACCTGC ACCTGC(4/8)
612 GCAGGT
CfoI 915 GCGC GCG^C
1836 GCGC
HhaI 915 GCGC GCG^C
1836 GCGC
HinP1I 915 GCGC G^CGC
1836 GCGC
NcoI 3054 CCATGG C^CATGG
3240 CCATGG
PflMI 1608 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3054 CCATGGCTTGG
PpuMI 969 AGGTCCC [GA]G^G[AT]CC[CT]
3547 GGGACCC
RcaI 596 TCATGA T^CATGA
1505 TCATGA
SexAI 1550 ACCAGGT A^CC[AT]GGT
1721 ACCTGGT
Van91I 1608 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3054 CCATGGCTTGG
XcmI 1142 CCATGACCGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCACGGCCTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrBI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII