##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I ApaLI AscI Asp700I
Asp718I AspI AviII BbrPI BbvCI
BclI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
BssSI BstAPI BstEII BstPI ClaI
CpoI EcoO65I EcoRV FbaI FseI
FspI HindIII HpaI KpnI MluI
MroI NcoI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SalI SgrAI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 1244 CTTAAG C^TTAAG
AseI 3381 ATTAAT AT^TAAT
AsnI 3381 ATTAAT AT^TAAT
AvrII 3224 CCTAGG C^CTAGG
BamHI 578 GGATCC G^GATCC
BcgI 352 GCAGCGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1244 CTTAAG C^TTAAG
BlnI 3224 CCTAGG C^CTAGG
BlpI 1505 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1505 GCTCAGC GC^T[ACGT]AGC
BsgI 902 GTGCAG GTGCAG(16/14)
BsmI 2682 GAATGC GAATGC(1/-1)
Bsp1407I 1576 TGTACA T^GTACA
BspHI 3133 TCATGA T^CATGA
BsrDI 808 CATTGC GCAATG(2/0)
BsrGI 1576 TGTACA T^GTACA
Bst1107I 3565 GTATAC GTA^TAC
BstBI 1252 TTCGAA TT^CGAA
BstXI 2277 CCACCGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3565 GTATAC GTA^TAC
CelII 1505 GCTCAGC GC^T[ACGT]AGC
DraIII 3215 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1480 GACAGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1249 GAATTC G^AATTC
EcoT22I 3577 ATGCAT ATGCA^T
HincII 3450 GTTGAC GT[CT]^[GA]AC
HindII 3450 GTTGAC GT[CT]^[GA]AC
MfeI 410 CAATTG C^AATTG
MunI 410 CAATTG C^AATTG
NaeI 118 GCCGGC GCC^GGC
NdeI 3689 CATATG CA^TATG
NgoMIV 118 GCCGGC G^CCGGC
NheI 2231 GCTAGC G^CTAGC
NsiI 3577 ATGCAT ATGCA^T
NspV 1252 TTCGAA TT^CGAA
PpuMI 3181 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3381 ATTAAT AT^TAAT
PvuII 2834 CAGCTG CAG^CTG
RcaI 3133 TCATGA T^CATGA
SbfI 1612 CCTGCAGG CCTGCA^GG
SexAI 1728 ACCTGGT A^CC[AT]GGT
SfiI 217 GGCCACGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1252 TTCGAA TT^CGAA
Sse8387I 1612 CCTGCAGG CCTGCA^GG
SspBI 1576 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Aor51HI 1502 AGCGCT AGC^GCT
3431 AGCGCT
ApaI 1979 GGGCCC GGGCC^C
2148 GGGCCC
AspHI 515 GAGCAC G[AT]GC[AT]^C
2377 GAGCAC
BalI 2044 TGGCCA TGG^CCA
2067 TGGCCA
BbsI 743 GAAGAC GAAGAC(2/6)
2967 GTCTTC
BglII 703 AGATCT A^GATCT
2908 AGATCT
BpuAI 743 GAAGAC GAAGAC(2/6)
2967 GTCTTC
BsaAI 2225 TACGTG [CT]AC^GT[GA]
2732 CACGTA
BsaBI 815 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
863 GATGTATATC
BsiHKAI 515 GAGCAC G[AT]GC[AT]^C
2377 GAGCAC
BsrBI 188 GAGCGG CCGCTC(-3/-3)
206 GAGCGG
BsrFI 118 GCCGGC [GA]^CCGG[CT]
2097 ACCGGC
Cfr10I 118 GCCGGC [GA]^CCGG[CT]
2097 ACCGGC
DraI 2690 TTTAAA TTT^AAA
2848 TTTAAA
Eco47III 1502 AGCGCT AGC^GCT
3431 AGCGCT
HgaI 1620 GCGTC GACGC(5/10)
1800 GCGTC
HphI 40 GGTGA GGTGA(8/7)
2811 GGTGA
KspI 1828 CCGCGG CCGC^GG
2007 CCGCGG
MaeII 2226 ACGT A^CGT
2733 ACGT
MamI 815 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
863 GATGTATATC
MluNI 2044 TGGCCA TGG^CCA
2067 TGGCCA
MscI 2044 TGGCCA TGG^CCA
2067 TGGCCA
NspI 1456 ACATGC [GA]CATG^[CT]
3579 GCATGT
PflMI 1708 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1843 CCAGGGGCTGG
PstI 699 CTGCAG CTGCA^G
1613 CTGCAG
SacII 1828 CCGCGG CCGC^GG
2007 CCGCGG
SapI 512 GAAGAGC GCTCTTC(1/4)
1701 GCTCTTC
ScaI 2244 AGTACT AGT^ACT
3598 AGTACT
Van91I 1708 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1843 CCAGGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApoI AspEI AvaI AvaII
BanI BanII BbeI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI