##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII AvrII BcgI BglI
BlnI BlpI Bpu1102I BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI BstEII BstPI CelII Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FseI FspI HindIII KspI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI PacI
PaeR7I PflFI PinAI PmeI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 2937 GGTACC G^GTACC
AclI 383 AACGTT AA^CGTT
AcyI 274 GGCGCC G[GA]^CG[CT]C
AocI 880 CCTGAGG CC^T[ACGT]AGG
Asp700I 3716 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2937 GGTACC G^GTACC
BalI 2233 TGGCCA TGG^CCA
BamHI 246 GGATCC G^GATCC
BbeI 274 GGCGCC GGCGC^C
BclI 3638 TGATCA T^GATCA
BglII 855 AGATCT A^GATCT
BsaBI 856 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 274 GGCGCC G[GA]^CG[CT]C
BsaI 1865 GGTCTC GGTCTC(1/5)
BsaWI 1328 ACCGGA [AT]^CCGG[AT]
BspHI 2085 TCATGA T^CATGA
BspLU11I 2309 ACATGT A^CATGT
BspMI 3027 ACCTGC ACCTGC(4/8)
BsrBI 1633 GAGCGG CCGCTC(-3/-3)
Bst1107I 2313 GTATAC GTA^TAC
BstAPI 1756 GCAAAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2313 GTATAC GTA^TAC
Bsu36I 880 CCTGAGG CC^T[ACGT]AGG
EaeI 2233 TGGCCA [CT]^GGCC[GA]
Eco81I 880 CCTGAGG CC^T[ACGT]AGG
EcoRI 2337 GAATTC G^AATTC
FbaI 3638 TGATCA T^GATCA
HgaI 182 GCGTC GACGC(5/10)
Hin1I 274 GGCGCC G[GA]^CG[CT]C
KasI 274 GGCGCC G^GCGCC
KpnI 2937 GGTACC GGTAC^C
MamI 856 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2233 TGGCCA TGG^CCA
MscI 2233 TGGCCA TGG^CCA
NarI 274 GGCGCC GG^CGCC
NruI 1317 TCGCGA TCG^CGA
PshAI 1457 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 383 AACGTT AA^CGTT
RcaI 2085 TCATGA T^CATGA
SapI 294 GAAGAGC GCTCTTC(1/4)
ScaI 2194 AGTACT AGT^ACT
SfoI 274 GGCGCC GGC^GCC
SphI 299 GCATGC GCATG^C
StuI 2393 AGGCCT AGG^CCT
XbaI 2992 TCTAGA T^CTAGA
XcmI 2162 CCACTTCCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3716 GAATGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 2313 GTATAC GT^[AC][GT]AC
3666 GTCTAC
AccII 1318 CGCG CG^CG
2330 CGCG
AflII 3090 CTTAAG C^TTAAG
3144 CTTAAG
BbrPI 1860 CACGTG CAC^GTG
2256 CACGTG
BbvCI 1830 CCTCAGC CCTCAGC(-5/-2)
2246 CCTCAGC
BfrI 3090 CTTAAG C^TTAAG
3144 CTTAAG
BsmI 2280 GCATTC GAATGC(1/-1)
2854 GCATTC
BstBI 1058 TTCGAA TT^CGAA
1138 TTCGAA
BstUI 1318 CGCG CG^CG
2330 CGCG
BstYI 246 GGATCC [GA]^GATC[CT]
855 AGATCT
DraIII 3304 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3647 CACTCAGTG
EcoNI 876 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1928 CCTGTATGAGG
EcoT22I 301 ATGCAT ATGCA^T
550 ATGCAT
FauI 2228 CCCGC CCCGC(4/6)
1635 GCGGG
HaeII 274 GGCGCC [GA]GCGC^[CT]
1546 AGCGCC
HpaI 639 GTTAAC GTT^AAC
3260 GTTAAC
MflI 246 GGATCC [GA]^GATC[CT]
855 AGATCT
MvnI 1318 CGCG CG^CG
2330 CGCG
NsiI 301 ATGCAT ATGCA^T
550 ATGCAT
NspV 1058 TTCGAA TT^CGAA
1138 TTCGAA
PflMI 1242 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1365 CCATCTCTTGG
PmaCI 1860 CACGTG CAC^GTG
2256 CACGTG
PmlI 1860 CACGTG CAC^GTG
2256 CACGTG
PstI 1024 CTGCAG CTGCA^G
1679 CTGCAG
PvuII 1904 CAGCTG CAG^CTG
1921 CAGCTG
SfuI 1058 TTCGAA TT^CGAA
1138 TTCGAA
Van91I 1242 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1365 CCATCTCTTGG
XhoII 246 GGATCC [GA]^GATC[CT]
855 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PshBI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I