################################## # NO CUT ENZYMES ( 57 enzymes) # ################################## AatII Acc65I AccIII AflII AflIII AhdI AscI Asp718I AspEI AspI AviII BamHI BciVI BclI BfrI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI MamI MluI MroI NdeI NotI NruI NsiI NspV PacI PflFI PmeI PvuI SalI ScaI SfuI SnaBI SpeI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 3011 GTCTAC GT^[AC][GT]AC AclI 1393 AACGTT AA^CGTT AcsI 824 AAATTC [GA]^AATT[CT] AcyI 32 GGCGCC G[GA]^CG[CT]C AgeI 3178 ACCGGT A^CCGGT Alw44I 2999 GTGCAC G^TGCAC Aor51HI 2945 AGCGCT AGC^GCT ApaLI 2999 GTGCAC G^TGCAC ApoI 824 AAATTC [GA]^AATT[CT] AseI 3402 ATTAAT AT^TAAT AsnI 3402 ATTAAT AT^TAAT Asp700I 1462 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 32 GGCGCC GGCGC^C BbrPI 1456 CACGTG CAC^GTG BcgI 2481 GCAAAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1624 AGATCT A^GATCT BsaHI 32 GGCGCC G[GA]^CG[CT]C Bsp1407I 1302 TGTACA T^GTACA BspHI 879 TCATGA T^CATGA BsrGI 1302 TGTACA T^GTACA BssSI 497 CACGAG CACGAG(-5/-1) BstEII 2149 GGTCACC G^GT[ACGT]ACC BstPI 2149 GGTCACC G^GT[ACGT]ACC BstXI 88 CCAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CpoI 3078 CGGACCG CG^G[AT]CCG DraI 891 TTTAAA TTT^AAA DraIII 2696 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2945 AGCGCT AGC^GCT EcoNI 82 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2149 GGTCACC G^GT[ACGT]ACC Hin1I 32 GGCGCC G[GA]^CG[CT]C HindIII 466 AAGCTT A^AGCTT KasI 32 GGCGCC G^GCGCC KspI 2928 CCGCGG CCGC^GG NaeI 1956 GCCGGC GCC^GGC NarI 32 GGCGCC GG^CGCC NcoI 1750 CCATGG C^CATGG NgoMIV 1956 GCCGGC G^CCGGC NheI 2242 GCTAGC G^CTAGC PinAI 3178 ACCGGT A^CCGGT PmaCI 1456 CACGTG CAC^GTG PmlI 1456 CACGTG CAC^GTG PshAI 1898 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3402 ATTAAT AT^TAAT Psp1406I 1393 AACGTT AA^CGTT RcaI 879 TCATGA T^CATGA RsrII 3078 CGGACCG CG^G[AT]CCG SacI 1853 GAGCTC GAGCT^C SacII 2928 CCGCGG CCGC^GG SfiI 485 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 32 GGCGCC GGC^GCC SgrAI 3177 CACCGGTG C[GA]^CCGG[CT]G SphI 3184 GCATGC GCATG^C SspBI 1302 TGTACA T^GTACA SspI 1329 AATATT AAT^ATT XbaI 223 TCTAGA T^CTAGA XmnI 1462 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 1992 CGCG CG^CG 2929 CGCG AocI 124 CCTCAGG CC^T[ACGT]AGG 449 CCTGAGG AvrII 82 CCTAGG C^CTAGG 378 CCTAGG BlnI 82 CCTAGG C^CTAGG 378 CCTAGG BlpI 1762 GCTGAGC GC^T[ACGT]AGC 2214 GCTTAGC Bpu1102I 1762 GCTGAGC GC^T[ACGT]AGC 2214 GCTTAGC BsaAI 689 CACGTA [CT]AC^GT[GA] 1456 CACGTG BsaWI 63 TCCGGT [AT]^CCGG[AT] 3178 ACCGGT BseRI 2300 GAGGAG GAGGAG(10/8) 3302 GAGGAG BsmBI 1196 GAGACG CGTCTC(1/5) 3331 GAGACG BsmI 2221 GAATGC GAATGC(1/-1) 684 GCATTC BsrBI 2113 CCGCTC CCGCTC(-3/-3) 1276 GAGCGG BstAPI 993 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1071 GCAGGCCCTGC BstUI 1992 CGCG CG^CG 2929 CGCG Bsu36I 124 CCTCAGG CC^T[ACGT]AGG 449 CCTGAGG CelII 1762 GCTGAGC GC^T[ACGT]AGC 2214 GCTTAGC Eco81I 124 CCTCAGG CC^T[ACGT]AGG 449 CCTGAGG MfeI 367 CAATTG C^AATTG 3414 CAATTG MunI 367 CAATTG C^AATTG 3414 CAATTG MvnI 1992 CGCG CG^CG 2929 CGCG NspI 930 ACATGC [GA]CATG^[CT] 3184 GCATGC PaeR7I 38 CTCGAG C^TCGAG 2297 CTCGAG PflMI 1654 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2466 CCATCGCCTGG SbfI 1077 CCTGCAGG CCTGCA^GG 1944 CCTGCAGG SexAI 2994 ACCTGGT A^CC[AT]GGT 3267 ACCTGGT SmaI 1013 CCCGGG CCC^GGG 2088 CCCGGG Sse8387I 1077 CCTGCAGG CCTGCA^GG 1944 CCTGCAGG Van91I 1654 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2466 CCATCGCCTGG XhoI 38 CTCGAG C^TCGAG 2297 CTCGAG XmaCI 1013 CCCGGG C^CCGGG 2088 CCCGGG XmaI 1013 CCCGGG C^CCGGG 2088 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII