##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AccIII AflII AflIII
AhdI AscI Asp718I AspEI AspI
AviII BamHI BciVI BclI BfrI
BsaBI BseAI BsiWI BspDI BspEI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI DrdI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI MamI MluI
MroI NdeI NotI NruI NsiI
NspV PacI PflFI PmeI PvuI
SalI ScaI SfuI SnaBI SpeI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 3011 GTCTAC GT^[AC][GT]AC
AclI 1393 AACGTT AA^CGTT
AcsI 824 AAATTC [GA]^AATT[CT]
AcyI 32 GGCGCC G[GA]^CG[CT]C
AgeI 3178 ACCGGT A^CCGGT
Alw44I 2999 GTGCAC G^TGCAC
Aor51HI 2945 AGCGCT AGC^GCT
ApaLI 2999 GTGCAC G^TGCAC
ApoI 824 AAATTC [GA]^AATT[CT]
AseI 3402 ATTAAT AT^TAAT
AsnI 3402 ATTAAT AT^TAAT
Asp700I 1462 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 32 GGCGCC GGCGC^C
BbrPI 1456 CACGTG CAC^GTG
BcgI 2481 GCAAAGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1624 AGATCT A^GATCT
BsaHI 32 GGCGCC G[GA]^CG[CT]C
Bsp1407I 1302 TGTACA T^GTACA
BspHI 879 TCATGA T^CATGA
BsrGI 1302 TGTACA T^GTACA
BssSI 497 CACGAG CACGAG(-5/-1)
BstEII 2149 GGTCACC G^GT[ACGT]ACC
BstPI 2149 GGTCACC G^GT[ACGT]ACC
BstXI 88 CCAGGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 3078 CGGACCG CG^G[AT]CCG
DraI 891 TTTAAA TTT^AAA
DraIII 2696 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2945 AGCGCT AGC^GCT
EcoNI 82 CCTAGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2149 GGTCACC G^GT[ACGT]ACC
Hin1I 32 GGCGCC G[GA]^CG[CT]C
HindIII 466 AAGCTT A^AGCTT
KasI 32 GGCGCC G^GCGCC
KspI 2928 CCGCGG CCGC^GG
NaeI 1956 GCCGGC GCC^GGC
NarI 32 GGCGCC GG^CGCC
NcoI 1750 CCATGG C^CATGG
NgoMIV 1956 GCCGGC G^CCGGC
NheI 2242 GCTAGC G^CTAGC
PinAI 3178 ACCGGT A^CCGGT
PmaCI 1456 CACGTG CAC^GTG
PmlI 1456 CACGTG CAC^GTG
PshAI 1898 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3402 ATTAAT AT^TAAT
Psp1406I 1393 AACGTT AA^CGTT
RcaI 879 TCATGA T^CATGA
RsrII 3078 CGGACCG CG^G[AT]CCG
SacI 1853 GAGCTC GAGCT^C
SacII 2928 CCGCGG CCGC^GG
SfiI 485 GGCCAGCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 32 GGCGCC GGC^GCC
SgrAI 3177 CACCGGTG C[GA]^CCGG[CT]G
SphI 3184 GCATGC GCATG^C
SspBI 1302 TGTACA T^GTACA
SspI 1329 AATATT AAT^ATT
XbaI 223 TCTAGA T^CTAGA
XmnI 1462 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1992 CGCG CG^CG
2929 CGCG
AocI 124 CCTCAGG CC^T[ACGT]AGG
449 CCTGAGG
AvrII 82 CCTAGG C^CTAGG
378 CCTAGG
BlnI 82 CCTAGG C^CTAGG
378 CCTAGG
BlpI 1762 GCTGAGC GC^T[ACGT]AGC
2214 GCTTAGC
Bpu1102I 1762 GCTGAGC GC^T[ACGT]AGC
2214 GCTTAGC
BsaAI 689 CACGTA [CT]AC^GT[GA]
1456 CACGTG
BsaWI 63 TCCGGT [AT]^CCGG[AT]
3178 ACCGGT
BseRI 2300 GAGGAG GAGGAG(10/8)
3302 GAGGAG
BsmBI 1196 GAGACG CGTCTC(1/5)
3331 GAGACG
BsmI 2221 GAATGC GAATGC(1/-1)
684 GCATTC
BsrBI 2113 CCGCTC CCGCTC(-3/-3)
1276 GAGCGG
BstAPI 993 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1071 GCAGGCCCTGC
BstUI 1992 CGCG CG^CG
2929 CGCG
Bsu36I 124 CCTCAGG CC^T[ACGT]AGG
449 CCTGAGG
CelII 1762 GCTGAGC GC^T[ACGT]AGC
2214 GCTTAGC
Eco81I 124 CCTCAGG CC^T[ACGT]AGG
449 CCTGAGG
MfeI 367 CAATTG C^AATTG
3414 CAATTG
MunI 367 CAATTG C^AATTG
3414 CAATTG
MvnI 1992 CGCG CG^CG
2929 CGCG
NspI 930 ACATGC [GA]CATG^[CT]
3184 GCATGC
PaeR7I 38 CTCGAG C^TCGAG
2297 CTCGAG
PflMI 1654 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2466 CCATCGCCTGG
SbfI 1077 CCTGCAGG CCTGCA^GG
1944 CCTGCAGG
SexAI 2994 ACCTGGT A^CC[AT]GGT
3267 ACCTGGT
SmaI 1013 CCCGGG CCC^GGG
2088 CCCGGG
Sse8387I 1077 CCTGCAGG CCTGCA^GG
1944 CCTGCAGG
Van91I 1654 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2466 CCATCGCCTGG
XhoI 38 CTCGAG C^TCGAG
2297 CTCGAG
XmaCI 1013 CCCGGG C^CCGGG
2088 CCCGGG
XmaI 1013 CCCGGG C^CCGGG
2088 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII