##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
BcgI BfrI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BspDI BspEI
Bst1107I BstAPI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SbfI
SexAI SfiI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1535 GACGTC GACGT^C
AcsI 2648 GAATTT [GA]^AATT[CT]
Alw44I 2938 GTGCAC G^TGCAC
ApaI 2914 GGGCCC GGGCC^C
ApaLI 2938 GTGCAC G^TGCAC
ApoI 2648 GAATTT [GA]^AATT[CT]
AvrII 488 CCTAGG C^CTAGG
BbeI 1460 GGCGCC GGCGC^C
BbrPI 1721 CACGTG CAC^GTG
BciVI 1514 GTATCC GTATCC(6/5)
BlnI 488 CCTAGG C^CTAGG
BsaWI 584 ACCGGA [AT]^CCGG[AT]
BsmBI 930 GAGACG CGTCTC(1/5)
BsmI 2007 GCATTC GAATGC(1/-1)
BspLU11I 2547 ACATGT A^CATGT
BsrBI 2763 CCGCTC CCGCTC(-3/-3)
BsrDI 2278 CATTGC GCAATG(2/0)
BssHII 128 GCGCGC G^CGCGC
BssSI 2904 CTCGTG CACGAG(-5/-1)
BstBI 2855 TTCGAA TT^CGAA
HaeII 1460 GGCGCC [GA]GCGC^[CT]
HindIII 3071 AAGCTT A^AGCTT
HpaI 2024 GTTAAC GTT^AAC
KasI 1460 GGCGCC G^GCGCC
KspI 829 CCGCGG CCGC^GG
NarI 1460 GGCGCC GG^CGCC
NcoI 429 CCATGG C^CATGG
NspV 2855 TTCGAA TT^CGAA
PmaCI 1721 CACGTG CAC^GTG
PmlI 1721 CACGTG CAC^GTG
SacII 829 CCGCGG CCGC^GG
SalI 1538 GTCGAC G^TCGAC
SapI 1426 GCTCTTC GCTCTTC(1/4)
SfoI 1460 GGCGCC GGC^GCC
SfuI 2855 TTCGAA TT^CGAA
SmaI 104 CCCGGG CCC^GGG
SphI 1413 GCATGC GCATG^C
SspI 1028 AATATT AAT^ATT
XmaCI 104 CCCGGG C^CCGGG
XmaI 104 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1538 GTCGAC GT^[AC][GT]AC
1697 GTCTAC
AflIII 2547 ACATGT A^C[GA][CT]GT
2739 ACACGT
AocI 211 CCTGAGG CC^T[ACGT]AGG
737 CCTGAGG
BalI 190 TGGCCA TGG^CCA
1665 TGGCCA
BamHI 825 GGATCC G^GATCC
1155 GGATCC
BbvCI 731 CCTCAGC CCTCAGC(-5/-2)
707 GCTGAGG
BclI 1941 TGATCA T^GATCA
2586 TGATCA
BglII 1636 AGATCT A^GATCT
2803 AGATCT
BsaI 588 GAGACC GGTCTC(1/5)
1475 GAGACC
BstEII 970 GGTCACC G^GT[ACGT]ACC
1957 GGTGACC
BstPI 970 GGTCACC G^GT[ACGT]ACC
1957 GGTGACC
Bsu36I 211 CCTGAGG CC^T[ACGT]AGG
737 CCTGAGG
DraIII 227 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
2417 CACCTTGTG
Eco81I 211 CCTGAGG CC^T[ACGT]AGG
737 CCTGAGG
EcoO65I 970 GGTCACC G^GT[ACGT]ACC
1957 GGTGACC
FbaI 1941 TGATCA T^GATCA
2586 TGATCA
MluNI 190 TGGCCA TGG^CCA
1665 TGGCCA
MscI 190 TGGCCA TGG^CCA
1665 TGGCCA
PpuMI 26 AGGTCCC [GA]G^G[AT]CC[CT]
2490 AGGTCCC
PstI 412 CTGCAG CTGCA^G
599 CTGCAG
SacI 4 GAGCTC GAGCT^C
392 GAGCTC
ScaI 321 AGTACT AGT^ACT
2436 AGTACT
SfcI 412 CTGCAG C^T[GA][CT]AG
599 CTGCAG
SmlI 1097 CTTGAG C^T[CT][GA]AG
2927 CTTGAG
StuI 864 AGGCCT AGG^CCT
913 AGGCCT
XcmI 1423 CCAGCTCTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1671 CCATTGAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII