##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbrPI BcgI BfrI
BglI BglII BlnI BsaAI BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI FseI
HindIII HpaI KasI KpnI KspI
MamI MluI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1993 GACGTC GACGT^C
AccIII 714 TCCGGA T^CCGGA
Alw44I 1453 GTGCAC G^TGCAC
AlwNI 300 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 496 GGGCCC GGGCC^C
ApaLI 1453 GTGCAC G^TGCAC
AvaI 1205 CCCGAG C^[CT]CG[GA]G
BanI 1471 GGCACC G^G[CT][GA]CC
BbvCI 411 CCTCAGC CCTCAGC(-5/-2)
BciVI 634 GTATCC GTATCC(6/5)
BlpI 991 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 991 GCTCAGC GC^T[ACGT]AGC
BseAI 714 TCCGGA T^CCGGA
BseRI 895 GAGGAG GAGGAG(10/8)
BsoBI 1205 CCCGAG C^[CT]CG[GA]G
BspEI 714 TCCGGA T^CCGGA
BspHI 2189 TCATGA T^CATGA
CelII 991 GCTCAGC GC^T[ACGT]AGC
DraI 1561 TTTAAA TTT^AAA
EcoRV 2515 GATATC GAT^ATC
EcoT22I 866 ATGCAT ATGCA^T
HaeII 1830 GGCGCT [GA]GCGC^[CT]
HincII 848 GTCAAC GT[CT]^[GA]AC
HindII 848 GTCAAC GT[CT]^[GA]AC
MfeI 2855 CAATTG C^AATTG
MroI 714 TCCGGA T^CCGGA
MunI 2855 CAATTG C^AATTG
NdeI 1289 CATATG CA^TATG
NsiI 866 ATGCAT ATGCA^T
PshAI 615 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1462 CTGCAG CTGCA^G
PvuII 2098 CAGCTG CAG^CTG
RcaI 2189 TCATGA T^CATGA
SapI 1270 GAAGAGC GCTCTTC(1/4)
ScaI 3231 AGTACT AGT^ACT
SmlI 436 CTCAAG C^T[CT][GA]AG
SspI 3192 AATATT AAT^ATT
XbaI 426 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 2895 AAATTC [GA]^AATT[CT]
2992 AAATTT
AcyI 538 GACGCC G[GA]^CG[CT]C
1993 GACGTC
AflIII 852 ACATGT A^C[GA][CT]GT
3077 ACATGT
ApoI 2895 AAATTC [GA]^AATT[CT]
2992 AAATTT
AviII 292 TGCGCA TGC^GCA
2004 TGCGCA
BalI 2115 TGGCCA TGG^CCA
2790 TGGCCA
BamHI 591 GGATCC G^GATCC
1569 GGATCC
BsaHI 538 GACGCC G[GA]^CG[CT]C
1993 GACGTC
BsaWI 714 TCCGGA [AT]^CCGG[AT]
1369 ACCGGA
BsgI 1278 CTGCAC GTGCAG(16/14)
2088 CTGCAC
BspLU11I 852 ACATGT A^CATGT
3077 ACATGT
BspMI 244 ACCTGC ACCTGC(4/8)
391 ACCTGC
BsrFI 395 GCCGGC [GA]^CCGG[CT]
1721 GCCGGC
BstAPI 1010 GCAAACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1455 GCACGGTCTGC
BstYI 591 GGATCC [GA]^GATC[CT]
1569 GGATCC
Cfr10I 395 GCCGGC [GA]^CCGG[CT]
1721 GCCGGC
DraII 272 AGGACCC [GA]G^G[ACGT]CC[CT]
566 AGGTCCT
DraIII 338 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2028 CACCGTGTG
EcoO109I 272 AGGACCC [GA]G^G[ACGT]CC[CT]
566 AGGTCCT
FspI 292 TGCGCA TGC^GCA
2004 TGCGCA
Hin1I 538 GACGCC G[GA]^CG[CT]C
1993 GACGTC
MflI 591 GGATCC [GA]^GATC[CT]
1569 GGATCC
MluNI 2115 TGGCCA TGG^CCA
2790 TGGCCA
MscI 2115 TGGCCA TGG^CCA
2790 TGGCCA
MspA1I 1839 CCGCTG C[AC]G^C[GT]G
2098 CAGCTG
NaeI 395 GCCGGC GCC^GGC
1721 GCCGGC
NcoI 1124 CCATGG C^CATGG
2532 CCATGG
NgoMIV 395 GCCGGC G^CCGGC
1721 GCCGGC
PpuMI 272 AGGACCC [GA]G^G[AT]CC[CT]
566 AGGTCCT
XcmI 350 CCAACGAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1677 CCAGGGGAGCTATGG
XhoII 591 GGATCC [GA]^GATC[CT]
1569 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I