##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BcgI BciVI
BfrI BglII BlnI BsaI BsiEI
BsiWI BsmBI BsrBI Bst1107I BstBI
BstZ17I CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FseI FspI HpaI KasI
MluI NarI NcoI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI SphI
SspI SwaI XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1377 GACGTC GACGT^C
Acc65I 2670 GGTACC G^GTACC
AcsI 706 AAATTC [GA]^AATT[CT]
AflIII 3011 ACATGT A^C[GA][CT]GT
Alw44I 1206 GTGCAC G^TGCAC
ApaLI 1206 GTGCAC G^TGCAC
ApoI 706 AAATTC [GA]^AATT[CT]
Asp718I 2670 GGTACC G^GTACC
BalI 2616 TGGCCA TGG^CCA
BbrPI 54 CACGTG CAC^GTG
BglI 746 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 54 CACGTG [CT]AC^GT[GA]
BsmI 3411 GCATTC GAATGC(1/-1)
Bsp1407I 2484 TGTACA T^GTACA
BspDI 3214 ATCGAT AT^CGAT
BspLU11I 3011 ACATGT A^CATGT
BsrGI 2484 TGTACA T^GTACA
BssHII 1328 GCGCGC G^CGCGC
BssSI 175 CACGAG CACGAG(-5/-1)
BstAPI 1800 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 993 GGTCACC G^GT[ACGT]ACC
BstPI 993 GGTCACC G^GT[ACGT]ACC
BstXI 1691 CCAAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3214 ATCGAT AT^CGAT
DraI 439 TTTAAA TTT^AAA
DsaI 1187 CCGCGG C^C[GA][CT]GG
EcoO65I 993 GGTCACC G^GT[ACGT]ACC
EcoRV 2349 GATATC GAT^ATC
HaeII 888 GGCGCT [GA]GCGC^[CT]
HincII 2417 GTCAAC GT[CT]^[GA]AC
HindII 2417 GTCAAC GT[CT]^[GA]AC
HindIII 2747 AAGCTT A^AGCTT
KpnI 2670 GGTACC GGTAC^C
KspI 1187 CCGCGG CCGC^GG
MfeI 2557 CAATTG C^AATTG
MluNI 2616 TGGCCA TGG^CCA
MscI 2616 TGGCCA TGG^CCA
MunI 2557 CAATTG C^AATTG
NaeI 22 GCCGGC GCC^GGC
NdeI 3362 CATATG CA^TATG
NgoMIV 22 GCCGGC G^CCGGC
PmaCI 54 CACGTG CAC^GTG
PmlI 54 CACGTG CAC^GTG
SacII 1187 CCGCGG CCGC^GG
SgrAI 21 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 598 CCCGGG CCC^GGG
SspBI 2484 TGTACA T^GTACA
XmaCI 598 CCCGGG C^CCGGG
XmaI 598 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 205 GTAGAC GT^[AC][GT]AC
1897 GTAGAC
AcyI 1377 GACGTC G[GA]^CG[CT]C
2008 GACGCC
AocI 1014 CCTGAGG CC^T[ACGT]AGG
3272 CCTCAGG
AspI 1243 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACAGAGTC
BamHI 1028 GGATCC G^GATCC
2144 GGATCC
BclI 152 TGATCA T^GATCA
2034 TGATCA
BsaHI 1377 GACGTC G[GA]^CG[CT]C
2008 GACGCC
BspHI 3163 TCATGA T^CATGA
3171 TCATGA
BsrDI 3290 CATTGC GCAATG(2/0)
2285 GCAATG
Bsu36I 1014 CCTGAGG CC^T[ACGT]AGG
3272 CCTCAGG
Eco81I 1014 CCTGAGG CC^T[ACGT]AGG
3272 CCTCAGG
FbaI 152 TGATCA T^GATCA
2034 TGATCA
Hin1I 1377 GACGTC G[GA]^CG[CT]C
2008 GACGCC
NspI 2042 ACATGC [GA]CATG^[CT]
3011 ACATGT
PflFI 1243 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACAGAGTC
RcaI 3163 TCATGA T^CATGA
3171 TCATGA
SapI 510 GAAGAGC GCTCTTC(1/4)
2242 GCTCTTC
StuI 639 AGGCCT AGG^CCT
3152 AGGCCT
Tth111I 1243 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1468 GACAGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PshAI PstI PvuII RsaI SacI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SmlI Sse8387I StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII