##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BsaAI BsaBI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FbaI
FseI FspI HpaI KasI MamI
MfeI MluI MluNI MroI MscI
MunI NarI NcoI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 2492 GGTACC G^GTACC
AccI 737 GTCGAC GT^[AC][GT]AC
AcsI 316 GAATTC [GA]^AATT[CT]
AflII 1149 CTTAAG C^TTAAG
AhdI 852 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 723 CCTGAGG CC^T[ACGT]AGG
ApaI 249 GGGCCC GGGCC^C
ApoI 316 GAATTC [GA]^AATT[CT]
Asp718I 2492 GGTACC G^GTACC
AspEI 852 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2144 GGATCC G^GATCC
BfrI 1149 CTTAAG C^TTAAG
BsaWI 2395 ACCGGA [AT]^CCGG[AT]
BspMI 1499 GCAGGT ACCTGC(4/8)
BsrBI 718 CCGCTC CCGCTC(-3/-3)
BsrFI 227 GCCGGC [GA]^CCGG[CT]
BstAPI 275 GCACAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1605 GGTGACC G^GT[ACGT]ACC
BstPI 1605 GGTGACC G^GT[ACGT]ACC
BstXI 1660 CCATAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 723 CCTGAGG CC^T[ACGT]AGG
Cfr10I 227 GCCGGC [GA]^CCGG[CT]
Eam1105I 852 GACCTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 723 CCTGAGG CC^T[ACGT]AGG
EcoNI 2167 CCTTGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1605 GGTGACC G^GT[ACGT]ACC
EcoRI 316 GAATTC G^AATTC
EcoT14I 2343 CCTTGG C^C[AT][AT]GG
HincII 737 GTCGAC GT[CT]^[GA]AC
HindII 737 GTCGAC GT[CT]^[GA]AC
HindIII 124 AAGCTT A^AGCTT
KpnI 2492 GGTACC GGTAC^C
KspI 840 CCGCGG CCGC^GG
NaeI 227 GCCGGC GCC^GGC
NgoMIV 227 GCCGGC G^CCGGC
PshAI 1779 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1746 GAGCTC GAGCT^C
SacII 840 CCGCGG CCGC^GG
SalI 737 GTCGAC G^TCGAC
SspI 1315 AATATT AAT^ATT
StuI 656 AGGCCT AGG^CCT
StyI 2343 CCTTGG C^C[AT][AT]GG
XcmI 2618 CCAGCATTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 1799 GGCGTC G[GA]^CG[CT]C
2034 GGCGTC
AflIII 994 ACATGT A^C[GA][CT]GT
2668 ACATGT
AspHI 273 GAGCAC G[AT]GC[AT]^C
1746 GAGCTC
AvaI 695 CTCGAG C^[CT]CG[GA]G
1790 CTCGAG
BbsI 515 GAAGAC GAAGAC(2/6)
758 GTCTTC
BglI 541 GCCCCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1212 GCCCAGTTGGC
BpuAI 515 GAAGAC GAAGAC(2/6)
758 GTCTTC
BsaHI 1799 GGCGTC G[GA]^CG[CT]C
2034 GGCGTC
BsaI 1917 GAGACC GGTCTC(1/5)
2419 GAGACC
BsiHKAI 273 GAGCAC G[AT]GC[AT]^C
1746 GAGCTC
BsoBI 695 CTCGAG C^[CT]CG[GA]G
1790 CTCGAG
BspLU11I 994 ACATGT A^CATGT
2668 ACATGT
BstYI 2072 GGATCT [GA]^GATC[CT]
2144 GGATCC
DpnII 2073 GATC ^GATC
2145 GATC
HaeII 433 GGCGCT [GA]GCGC^[CT]
1111 AGCGCC
HgaI 1800 GCGTC GACGC(5/10)
2035 GCGTC
Hin1I 1799 GGCGTC G[GA]^CG[CT]C
2034 GGCGTC
MaeII 1642 ACGT A^CGT
1717 ACGT
MboI 2073 GATC ^GATC
2145 GATC
MflI 2072 GGATCT [GA]^GATC[CT]
2144 GGATCC
NdeI 342 CATATG CA^TATG
732 CATATG
NdeII 2073 GATC ^GATC
2145 GATC
PaeR7I 695 CTCGAG C^TCGAG
1790 CTCGAG
PstI 1056 CTGCAG CTGCA^G
1764 CTGCAG
PvuII 879 CAGCTG CAG^CTG
1158 CAGCTG
SapI 1439 GAAGAGC GCTCTTC(1/4)
1494 GAAGAGC
Sau3AI 2073 GATC ^GATC
2145 GATC
ScaI 2047 AGTACT AGT^ACT
2124 AGTACT
SexAI 668 ACCAGGT A^CC[AT]GGT
2422 ACCTGGT
SphI 1168 GCATGC GCATG^C
2462 GCATGC
XhoI 695 CTCGAG C^TCGAG
1790 CTCGAG
XhoII 2072 GGATCT [GA]^GATC[CT]
2144 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr13I
DdeI DraI DraII DraIII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV NspI
PleI PpuMI RcaI RsaI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I