################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BseAI BsgI BsiWI Bsp1407I BspDI BspEI BspMI BsrGI BssSI BstAPI BstEII BstPI BstXI CelII ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MluNI MroI MscI MunI NcoI NheI NotI NruI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SgrAI SnaBI Sse8387I SspBI StyI SwaI Tth111I Van91I ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AatII 158 GACGTC GACGT^C ApaI 2628 GGGCCC GGGCC^C AspHI 1632 GAGCAC G[AT]GC[AT]^C BamHI 1382 GGATCC G^GATCC BanII 2628 GGGCCC G[GA]GC[CT]^C BbeI 75 GGCGCC GGCGC^C BsaAI 828 TACGTG [CT]AC^GT[GA] BsaBI 2978 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 259 TCCGGT [AT]^CCGG[AT] BsiEI 261 CGGTCG CG[GA][CT]^CG BsiHKAI 1632 GAGCAC G[AT]GC[AT]^C BspHI 2171 TCATGA T^CATGA BsrBI 425 GAGCGG CCGCTC(-3/-3) BsrDI 623 CATTGC GCAATG(2/0) Bst1107I 825 GTATAC GTA^TAC BstZ17I 825 GTATAC GTA^TAC DraI 2844 TTTAAA TTT^AAA EaeI 1436 TGGCCG [CT]^GGCC[GA] EcoT22I 2934 ATGCAT ATGCA^T HincII 40 GTCGAC GT[CT]^[GA]AC HindII 40 GTCGAC GT[CT]^[GA]AC KasI 75 GGCGCC G^GCGCC MamI 2978 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 75 GGCGCC GG^CGCC NdeI 2320 CATATG CA^TATG NsiI 2934 ATGCAT ATGCA^T PaeR7I 2130 CTCGAG C^TCGAG PstI 709 CTGCAG CTGCA^G PvuII 1856 CAGCTG CAG^CTG RcaI 2171 TCATGA T^CATGA SalI 40 GTCGAC G^TCGAC SfoI 75 GGCGCC GGC^GCC SmaI 88 CCCGGG CCC^GGG SpeI 2754 ACTAGT A^CTAGT StuI 1562 AGGCCT AGG^CCT Tsp45I 505 GTGAC ^GT[GC]AC XbaI 1611 TCTAGA T^CTAGA XcmI 1454 CCAGGCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2130 CTCGAG C^TCGAG XmaCI 88 CCCGGG C^CCGGG XmaI 88 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 40 GTCGAC GT^[AC][GT]AC 825 GTATAC AfaI 485 GTAC GT^AC 2832 GTAC AlwNI 1576 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 3011 CAGAAACTG AocI 1548 CCTGAGG CC^T[ACGT]AGG 1565 CCTTAGG BciVI 2315 GGATAC GTATCC(6/5) 2904 GGATAC BmyI 1632 GAGCAC G[AGT]GC[ACT]^C 2628 GGGCCC BpmI 362 CTCCAG CTGGAG(16/14) 844 CTCCAG BsaI 1169 GAGACC GGTCTC(1/5) 2127 GGTCTC BsmBI 490 GAGACG CGTCTC(1/5) 967 GAGACG BsmI 472 GAATGC GAATGC(1/-1) 1655 GAATGC Bsp1286I 1632 GAGCAC G[AGT]GC[ACT]^C 2628 GGGCCC BspLU11I 1055 ACATGT A^CATGT 2772 ACATGT BsrFI 107 GCCGGC [GA]^CCGG[CT] 522 GCCGGC BssHII 294 GCGCGC G^CGCGC 302 GCGCGC BstBI 1307 TTCGAA TT^CGAA 2511 TTCGAA Bsu36I 1548 CCTGAGG CC^T[ACGT]AGG 1565 CCTTAGG Cfr10I 107 GCCGGC [GA]^CCGG[CT] 522 GCCGGC Eco81I 1548 CCTGAGG CC^T[ACGT]AGG 1565 CCTTAGG HphI 504 GGTGA GGTGA(8/7) 2418 GGTGA MspA1I 55 CAGCGG C[AC]G^C[GT]G 1856 CAGCTG NaeI 107 GCCGGC GCC^GGC 522 GCCGGC NgoMIV 107 GCCGGC G^CCGGC 522 GCCGGC NspV 1307 TTCGAA TT^CGAA 2511 TTCGAA PpuMI 59 GGGTCCC [GA]G^G[AT]CC[CT] 577 GGGACCT RsaI 485 GTAC GT^AC 2832 GTAC SfuI 1307 TTCGAA TT^CGAA 2511 TTCGAA SphI 103 GCATGC GCATG^C 2936 GCATGC SspI 2178 AATATT AAT^ATT 2947 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcsI AcyI AflIII AluI AlwI ApoI Asp700I AvaI AvaII BanI BbsI BbvI BcnI BfaI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI