##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BbrPI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspMI
BsrGI BssSI BstAPI BstEII BstPI
BstXI CelII ClaI CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NcoI
NheI NotI NruI PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SgrAI SnaBI Sse8387I
SspBI StyI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 158 GACGTC GACGT^C
ApaI 2628 GGGCCC GGGCC^C
AspHI 1632 GAGCAC G[AT]GC[AT]^C
BamHI 1382 GGATCC G^GATCC
BanII 2628 GGGCCC G[GA]GC[CT]^C
BbeI 75 GGCGCC GGCGC^C
BsaAI 828 TACGTG [CT]AC^GT[GA]
BsaBI 2978 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 259 TCCGGT [AT]^CCGG[AT]
BsiEI 261 CGGTCG CG[GA][CT]^CG
BsiHKAI 1632 GAGCAC G[AT]GC[AT]^C
BspHI 2171 TCATGA T^CATGA
BsrBI 425 GAGCGG CCGCTC(-3/-3)
BsrDI 623 CATTGC GCAATG(2/0)
Bst1107I 825 GTATAC GTA^TAC
BstZ17I 825 GTATAC GTA^TAC
DraI 2844 TTTAAA TTT^AAA
EaeI 1436 TGGCCG [CT]^GGCC[GA]
EcoT22I 2934 ATGCAT ATGCA^T
HincII 40 GTCGAC GT[CT]^[GA]AC
HindII 40 GTCGAC GT[CT]^[GA]AC
KasI 75 GGCGCC G^GCGCC
MamI 2978 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 75 GGCGCC GG^CGCC
NdeI 2320 CATATG CA^TATG
NsiI 2934 ATGCAT ATGCA^T
PaeR7I 2130 CTCGAG C^TCGAG
PstI 709 CTGCAG CTGCA^G
PvuII 1856 CAGCTG CAG^CTG
RcaI 2171 TCATGA T^CATGA
SalI 40 GTCGAC G^TCGAC
SfoI 75 GGCGCC GGC^GCC
SmaI 88 CCCGGG CCC^GGG
SpeI 2754 ACTAGT A^CTAGT
StuI 1562 AGGCCT AGG^CCT
Tsp45I 505 GTGAC ^GT[GC]AC
XbaI 1611 TCTAGA T^CTAGA
XcmI 1454 CCAGGCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2130 CTCGAG C^TCGAG
XmaCI 88 CCCGGG C^CCGGG
XmaI 88 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 40 GTCGAC GT^[AC][GT]AC
825 GTATAC
AfaI 485 GTAC GT^AC
2832 GTAC
AlwNI 1576 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
3011 CAGAAACTG
AocI 1548 CCTGAGG CC^T[ACGT]AGG
1565 CCTTAGG
BciVI 2315 GGATAC GTATCC(6/5)
2904 GGATAC
BmyI 1632 GAGCAC G[AGT]GC[ACT]^C
2628 GGGCCC
BpmI 362 CTCCAG CTGGAG(16/14)
844 CTCCAG
BsaI 1169 GAGACC GGTCTC(1/5)
2127 GGTCTC
BsmBI 490 GAGACG CGTCTC(1/5)
967 GAGACG
BsmI 472 GAATGC GAATGC(1/-1)
1655 GAATGC
Bsp1286I 1632 GAGCAC G[AGT]GC[ACT]^C
2628 GGGCCC
BspLU11I 1055 ACATGT A^CATGT
2772 ACATGT
BsrFI 107 GCCGGC [GA]^CCGG[CT]
522 GCCGGC
BssHII 294 GCGCGC G^CGCGC
302 GCGCGC
BstBI 1307 TTCGAA TT^CGAA
2511 TTCGAA
Bsu36I 1548 CCTGAGG CC^T[ACGT]AGG
1565 CCTTAGG
Cfr10I 107 GCCGGC [GA]^CCGG[CT]
522 GCCGGC
Eco81I 1548 CCTGAGG CC^T[ACGT]AGG
1565 CCTTAGG
HphI 504 GGTGA GGTGA(8/7)
2418 GGTGA
MspA1I 55 CAGCGG C[AC]G^C[GT]G
1856 CAGCTG
NaeI 107 GCCGGC GCC^GGC
522 GCCGGC
NgoMIV 107 GCCGGC G^CCGGC
522 GCCGGC
NspV 1307 TTCGAA TT^CGAA
2511 TTCGAA
PpuMI 59 GGGTCCC [GA]G^G[AT]CC[CT]
577 GGGACCT
RsaI 485 GTAC GT^AC
2832 GTAC
SfuI 1307 TTCGAA TT^CGAA
2511 TTCGAA
SphI 103 GCATGC GCATG^C
2936 GCATGC
SspI 2178 AATATT AAT^ATT
2947 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AcyI AflIII
AluI AlwI ApoI Asp700I AvaI
AvaII BanI BbsI BbvI BcnI
BfaI BpuAI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII XmnI