##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbsI BciVI BclI BpuAI BsaBI
BsiEI BsiWI BsmBI BspDI BspLU11I
BsrFI BssHII BstBI Cfr10I ClaI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KspI MamI MfeI MluI MunI
NaeI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
SacII SalI SapI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 385 GTATAC GT^[AC][GT]AC
AccIII 1465 TCCGGA T^CCGGA
AcsI 2398 AAATTC [GA]^AATT[CT]
AflII 1124 CTTAAG C^TTAAG
ApaI 170 GGGCCC GGGCC^C
ApoI 2398 AAATTC [GA]^AATT[CT]
AspI 1385 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2601 CCTAGG C^CTAGG
BbeI 28 GGCGCC GGCGC^C
BbrPI 2509 CACGTG CAC^GTG
BbvCI 754 CCTCAGC CCTCAGC(-5/-2)
BcgI 1168 CGACAAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1124 CTTAAG C^TTAAG
BlnI 2601 CCTAGG C^CTAGG
BsaAI 2509 CACGTG [CT]AC^GT[GA]
BsaWI 1465 TCCGGA [AT]^CCGG[AT]
BseAI 1465 TCCGGA T^CCGGA
BsmI 2937 GCATTC GAATGC(1/-1)
Bsp1407I 2769 TGTACA T^GTACA
BspEI 1465 TCCGGA T^CCGGA
BspHI 846 TCATGA T^CATGA
BsrGI 2769 TGTACA T^GTACA
BssSI 275 CTCGTG CACGAG(-5/-1)
Bst1107I 385 GTATAC GTA^TAC
BstAPI 15 GCAGACGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1971 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 385 GTATAC GTA^TAC
CpoI 1485 CGGTCCG CG^G[AT]CCG
DraIII 1447 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1324 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 128 GACGC GACGC(5/10)
HindIII 1546 AAGCTT A^AGCTT
KasI 28 GGCGCC G^GCGCC
MaeII 2510 ACGT A^CGT
MroI 1465 TCCGGA T^CCGGA
MseI 1125 TTAA T^TAA
MslI 1750 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 28 GGCGCC GG^CGCC
PflFI 1385 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1762 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2509 CACGTG CAC^GTG
PmlI 2509 CACGTG CAC^GTG
PpuMI 1703 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 445 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 846 TCATGA T^CATGA
RsrII 1485 CGGTCCG CG^G[AT]CCG
SacI 1853 GAGCTC GAGCT^C
SbfI 1345 CCTGCAGG CCTGCA^GG
ScaI 2040 AGTACT AGT^ACT
SfoI 28 GGCGCC GGC^GCC
Sse8387I 1345 CCTGCAGG CCTGCA^GG
SspBI 2769 TGTACA T^GTACA
StuI 2519 AGGCCT AGG^CCT
Tru9I 1125 TTAA T^TAA
Tth111I 1385 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1762 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 331 GGTACC G^GTACC
1683 GGTACC
AccII 407 CGCG CG^CG
1250 CGCG
AcyI 28 GGCGCC G[GA]^CG[CT]C
128 GACGCC
AocI 793 CCTGAGG CC^T[ACGT]AGG
2136 CCTTAGG
Asp718I 331 GGTACC G^GTACC
1683 GGTACC
AspHI 1812 GTGCTC G[AT]GC[AT]^C
1853 GAGCTC
BlpI 622 GCTGAGC GC^T[ACGT]AGC
2409 GCTCAGC
Bpu1102I 622 GCTGAGC GC^T[ACGT]AGC
2409 GCTCAGC
BsaHI 28 GGCGCC G[GA]^CG[CT]C
128 GACGCC
BsiHKAI 1812 GTGCTC G[AT]GC[AT]^C
1853 GAGCTC
BsrBI 394 CCGCTC CCGCTC(-3/-3)
1079 CCGCTC
BstEII 1900 GGTCACC G^GT[ACGT]ACC
2606 GGTCACC
BstPI 1900 GGTCACC G^GT[ACGT]ACC
2606 GGTCACC
BstUI 407 CGCG CG^CG
1250 CGCG
Bsu36I 793 CCTGAGG CC^T[ACGT]AGG
2136 CCTTAGG
CelII 622 GCTGAGC GC^T[ACGT]AGC
2409 GCTCAGC
Eco81I 793 CCTGAGG CC^T[ACGT]AGG
2136 CCTTAGG
EcoO65I 1900 GGTCACC G^GT[ACGT]ACC
2606 GGTCACC
HaeII 28 GGCGCC [GA]GCGC^[CT]
1476 AGCGCC
Hin1I 28 GGCGCC G[GA]^CG[CT]C
128 GACGCC
KpnI 331 GGTACC GGTAC^C
1683 GGTACC
MvnI 407 CGCG CG^CG
1250 CGCG
NspI 340 GCATGT [GA]CATG^[CT]
1113 ACATGC
SexAI 1767 ACCTGGT A^CC[AT]GGT
1934 ACCTGGT
TfiI 2335 GAATC G^A[AT]TC
3007 GATTC
Tsp509I 2399 AATT ^AATT
2964 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NdeII NheI NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII
XmaCI XmaI