##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BalI BbeI BcgI
BglI BlnI BlpI Bpu1102I BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI FseI
FspI HaeIII HgaI Hin1I HpaI
KasI KpnI MluI MluNI MroI
MscI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PvuI RsrII SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
StuI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 3555 GTAGAC GT^[AC][GT]AC
AclI 1810 AACGTT AA^CGTT
AflIII 1734 ACATGT A^C[GA][CT]GT
AseI 2703 ATTAAT AT^TAAT
AsnI 2703 ATTAAT AT^TAAT
Asp700I 1449 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 62 GTGCTC G[AT]GC[AT]^C
AspI 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 83 CTCGAG C^[CT]CG[GA]G
BamHI 326 GGATCC G^GATCC
BanI 3379 GGTGCC G^G[CT][GA]CC
BbrPI 799 CACGTG CAC^GTG
BpmI 916 CTGGAG CTGGAG(16/14)
BsaBI 3685 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 122 TCCGGT [AT]^CCGG[AT]
BsiHKAI 62 GTGCTC G[AT]GC[AT]^C
BsmI 1692 GCATTC GAATGC(1/-1)
BsoBI 83 CTCGAG C^[CT]CG[GA]G
BspHI 2783 TCATGA T^CATGA
BspLU11I 1734 ACATGT A^CATGT
BspMI 3297 GCAGGT ACCTGC(4/8)
DraIII 799 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3273 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2062 GATATC GAT^ATC
FauI 168 GCGGG CCCGC(4/6)
HincII 3319 GTCAAC GT[CT]^[GA]AC
HindII 3319 GTCAAC GT[CT]^[GA]AC
KspI 138 CCGCGG CCGC^GG
MamI 3685 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 418 CAATTG C^AATTG
MunI 418 CAATTG C^AATTG
NdeI 1153 CATATG CA^TATG
PaeR7I 83 CTCGAG C^TCGAG
PflFI 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1152 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 799 CACGTG CAC^GTG
PmlI 799 CACGTG CAC^GTG
PshAI 2157 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2703 ATTAAT AT^TAAT
Psp1406I 1810 AACGTT AA^CGTT
PvuII 2680 CAGCTG CAG^CTG
RcaI 2783 TCATGA T^CATGA
SacII 138 CCGCGG CCGC^GG
SapI 3757 GAAGAGC GCTCTTC(1/4)
ScaI 3017 AGTACT AGT^ACT
SnaBI 2410 TACGTA TAC^GTA
Tsp45I 1731 GTGAC ^GT[GC]AC
Tth111I 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1152 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 729 TCTAGA T^CTAGA
XhoI 83 CTCGAG C^TCGAG
XmnI 1449 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 139 CGCG CG^CG
197 CGCG
AflII 245 CTTAAG C^TTAAG
2055 CTTAAG
BanII 481 GGGCTC G[GA]GC[CT]^C
1433 GAGCCC
BciVI 3011 GTATCC GTATCC(6/5)
3610 GTATCC
BclI 2698 TGATCA T^GATCA
3114 TGATCA
BfrI 245 CTTAAG C^TTAAG
2055 CTTAAG
BsaAI 799 CACGTG [CT]AC^GT[GA]
2410 TACGTA
Bsp1407I 1862 TGTACA T^GTACA
2894 TGTACA
BsrGI 1862 TGTACA T^GTACA
2894 TGTACA
BsrI 2203 CCAGT ACTGG(1/-1)
3015 CCAGT
BstUI 139 CGCG CG^CG
197 CGCG
BstXI 1785 CCAGGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3614 CCAAATACTTGG
Cac8I 333 GCAAGC GC[ACGT]^[ACGT]GC
2688 GCAAGC
CfoI 42 GCGC GCG^C
109 GCGC
EcoT22I 1502 ATGCAT ATGCA^T
3727 ATGCAT
FbaI 2698 TGATCA T^GATCA
3114 TGATCA
HaeII 41 AGCGCC [GA]GCGC^[CT]
108 AGCGCC
HhaI 42 GCGC GCG^C
109 GCGC
HinP1I 42 GCGC G^CGC
109 GCGC
MvnI 139 CGCG CG^CG
197 CGCG
NsiI 1502 ATGCAT ATGCA^T
3727 ATGCAT
NspI 1734 ACATGT [GA]CATG^[CT]
2897 ACATGC
PstI 1296 CTGCAG CTGCA^G
3295 CTGCAG
SspBI 1862 TGTACA T^GTACA
2894 TGTACA
SspI 3666 AATATT AAT^ATT
3738 AATATT
TaqI 84 TCGA T^CGA
1465 TCGA
TthHB8I 84 TCGA T^CGA
1465 TCGA
XcmI 686 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1554 CCATTTTGTGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvCI BbvI
BcnI BfaI BglII BmyI BpuAI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII