################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccIII AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BalI BbeI BcgI BglI BlnI BlpI Bpu1102I BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI FseI FspI HaeIII HgaI Hin1I HpaI KasI KpnI MluI MluNI MroI MscI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PvuI RsrII SacI SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 3555 GTAGAC GT^[AC][GT]AC AclI 1810 AACGTT AA^CGTT AflIII 1734 ACATGT A^C[GA][CT]GT AseI 2703 ATTAAT AT^TAAT AsnI 2703 ATTAAT AT^TAAT Asp700I 1449 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 62 GTGCTC G[AT]GC[AT]^C AspI 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 83 CTCGAG C^[CT]CG[GA]G BamHI 326 GGATCC G^GATCC BanI 3379 GGTGCC G^G[CT][GA]CC BbrPI 799 CACGTG CAC^GTG BpmI 916 CTGGAG CTGGAG(16/14) BsaBI 3685 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 122 TCCGGT [AT]^CCGG[AT] BsiHKAI 62 GTGCTC G[AT]GC[AT]^C BsmI 1692 GCATTC GAATGC(1/-1) BsoBI 83 CTCGAG C^[CT]CG[GA]G BspHI 2783 TCATGA T^CATGA BspLU11I 1734 ACATGT A^CATGT BspMI 3297 GCAGGT ACCTGC(4/8) DraIII 799 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 3273 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2062 GATATC GAT^ATC FauI 168 GCGGG CCCGC(4/6) HincII 3319 GTCAAC GT[CT]^[GA]AC HindII 3319 GTCAAC GT[CT]^[GA]AC KspI 138 CCGCGG CCGC^GG MamI 3685 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 418 CAATTG C^AATTG MunI 418 CAATTG C^AATTG NdeI 1153 CATATG CA^TATG PaeR7I 83 CTCGAG C^TCGAG PflFI 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1152 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 799 CACGTG CAC^GTG PmlI 799 CACGTG CAC^GTG PshAI 2157 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2703 ATTAAT AT^TAAT Psp1406I 1810 AACGTT AA^CGTT PvuII 2680 CAGCTG CAG^CTG RcaI 2783 TCATGA T^CATGA SacII 138 CCGCGG CCGC^GG SapI 3757 GAAGAGC GCTCTTC(1/4) ScaI 3017 AGTACT AGT^ACT SnaBI 2410 TACGTA TAC^GTA Tsp45I 1731 GTGAC ^GT[GC]AC Tth111I 188 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1152 CCATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 729 TCTAGA T^CTAGA XhoI 83 CTCGAG C^TCGAG XmnI 1449 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 139 CGCG CG^CG 197 CGCG AflII 245 CTTAAG C^TTAAG 2055 CTTAAG BanII 481 GGGCTC G[GA]GC[CT]^C 1433 GAGCCC BciVI 3011 GTATCC GTATCC(6/5) 3610 GTATCC BclI 2698 TGATCA T^GATCA 3114 TGATCA BfrI 245 CTTAAG C^TTAAG 2055 CTTAAG BsaAI 799 CACGTG [CT]AC^GT[GA] 2410 TACGTA Bsp1407I 1862 TGTACA T^GTACA 2894 TGTACA BsrGI 1862 TGTACA T^GTACA 2894 TGTACA BsrI 2203 CCAGT ACTGG(1/-1) 3015 CCAGT BstUI 139 CGCG CG^CG 197 CGCG BstXI 1785 CCAGGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3614 CCAAATACTTGG Cac8I 333 GCAAGC GC[ACGT]^[ACGT]GC 2688 GCAAGC CfoI 42 GCGC GCG^C 109 GCGC EcoT22I 1502 ATGCAT ATGCA^T 3727 ATGCAT FbaI 2698 TGATCA T^GATCA 3114 TGATCA HaeII 41 AGCGCC [GA]GCGC^[CT] 108 AGCGCC HhaI 42 GCGC GCG^C 109 GCGC HinP1I 42 GCGC G^CGC 109 GCGC MvnI 139 CGCG CG^CG 197 CGCG NsiI 1502 ATGCAT ATGCA^T 3727 ATGCAT NspI 1734 ACATGT [GA]CATG^[CT] 2897 ACATGC PstI 1296 CTGCAG CTGCA^G 3295 CTGCAG SspBI 1862 TGTACA T^GTACA 2894 TGTACA SspI 3666 AATATT AAT^ATT 3738 AATATT TaqI 84 TCGA T^CGA 1465 TCGA TthHB8I 84 TCGA T^CGA 1465 TCGA XcmI 686 CCATACCATCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1554 CCATTTTGTGAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbsI BbvCI BbvI BcnI BfaI BglII BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII