##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AvrII BbeI
BbrPI BcgI BlnI BsaWI BseAI
BsiWI BsmI BspEI BssHII BssSI
BstBI BstEII BstPI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII KasI MfeI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2203 GGTACC G^GTACC
AccI 2528 GTATAC GT^[AC][GT]AC
AcyI 540 GACGCC G[GA]^CG[CT]C
AflII 2411 CTTAAG C^TTAAG
AocI 740 CCTGAGG CC^T[ACGT]AGG
ApaI 1007 GGGCCC GGGCC^C
Asp718I 2203 GGTACC G^GTACC
AviII 922 TGCGCA TGC^GCA
BamHI 1483 GGATCC G^GATCC
BciVI 1378 GTATCC GTATCC(6/5)
BfrI 2411 CTTAAG C^TTAAG
BglII 2138 AGATCT A^GATCT
BsaHI 540 GACGCC G[GA]^CG[CT]C
BsiEI 639 CGGCCG CG[GA][CT]^CG
BsmBI 4 CGTCTC CGTCTC(1/5)
BspHI 2344 TCATGA T^CATGA
BspLU11I 3360 ACATGT A^CATGT
BsrBI 1530 GAGCGG CCGCTC(-3/-3)
BsrDI 2357 CATTGC GCAATG(2/0)
BsrFI 1944 GCCGGT [GA]^CCGG[CT]
Bst1107I 2528 GTATAC GTA^TAC
BstAPI 1358 GCAGCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 157 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2528 GTATAC GTA^TAC
Bsu36I 740 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1944 GCCGGT [GA]^CCGG[CT]
CpoI 1912 CGGACCG CG^G[AT]CCG
DraIII 826 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2216 GACAGTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 639 CGGCCG C^GGCCG
EclXI 639 CGGCCG C^GGCCG
Eco52I 639 CGGCCG C^GGCCG
Eco81I 740 CCTGAGG CC^T[ACGT]AGG
EcoNI 527 CCTGGATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 922 TGCGCA TGC^GCA
Hin1I 540 GACGCC G[GA]^CG[CT]C
HpaI 3586 GTTAAC GTT^AAC
KpnI 2203 GGTACC GGTAC^C
MluI 1519 ACGCGT A^CGCGT
NcoI 1657 CCATGG C^CATGG
NdeI 3591 CATATG CA^TATG
PflMI 1046 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2448 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 2344 TCATGA T^CATGA
RsrII 1912 CGGACCG CG^G[AT]CCG
SapI 412 GCTCTTC GCTCTTC(1/4)
ScaI 2165 AGTACT AGT^ACT
SexAI 2874 ACCAGGT A^CC[AT]GGT
SphI 1918 GCATGC GCATG^C
StuI 1792 AGGCCT AGG^CCT
Van91I 1046 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Asp700I 1250 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1790 GAAGGCCTTC
AvaI 1710 CTCGGG C^[CT]CG[GA]G
1780 CTCGGG
BbvCI 2389 CCTCAGC CCTCAGC(-5/-2)
1140 GCTGAGG
BclI 2042 TGATCA T^GATCA
3515 TGATCA
BlpI 1835 GCTGAGC GC^T[ACGT]AGC
3021 GCTCAGC
Bpu1102I 1835 GCTGAGC GC^T[ACGT]AGC
3021 GCTCAGC
BsaI 366 GAGACC GGTCTC(1/5)
2438 GAGACC
BsoBI 1710 CTCGGG C^[CT]CG[GA]G
1780 CTCGGG
Bsp1407I 131 TGTACA T^GTACA
3404 TGTACA
BspDI 141 ATCGAT AT^CGAT
1017 ATCGAT
BspMI 724 ACCTGC ACCTGC(4/8)
775 ACCTGC
BsrGI 131 TGTACA T^GTACA
3404 TGTACA
CelII 1835 GCTGAGC GC^T[ACGT]AGC
3021 GCTCAGC
ClaI 141 ATCGAT AT^CGAT
1017 ATCGAT
FbaI 2042 TGATCA T^GATCA
3515 TGATCA
HaeII 664 AGCGCC [GA]GCGC^[CT]
1849 AGCGCC
HincII 2117 GTTGAC GT[CT]^[GA]AC
3586 GTTAAC
HindII 2117 GTTGAC GT[CT]^[GA]AC
3586 GTTAAC
KspI 16 CCGCGG CCGC^GG
57 CCGCGG
SacI 471 GAGCTC GAGCT^C
813 GAGCTC
SacII 16 CCGCGG CCGC^GG
57 CCGCGG
SspBI 131 TGTACA T^GTACA
3404 TGTACA
XbaI 3038 TCTAGA T^CTAGA
3377 TCTAGA
XcmI 1015 CCATCGATTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1765 CCAGCACCCCTGTGG
XmnI 1250 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1790 GAAGGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII