################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspHI AspI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspMI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI HapII HgaI Hin1I HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 2728 ACATGT A^C[GA][CT]GT AhdI 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 1893 ATTAAT AT^TAAT AsnI 1893 ATTAAT AT^TAAT Asp700I 3093 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 2264 TGCGCA TGC^GCA BalI 352 TGGCCA TGG^CCA BanI 1322 GGTGCC G^G[CT][GA]CC BanII 310 GAGCCC G[GA]GC[CT]^C BbsI 1540 GAAGAC GAAGAC(2/6) BmyI 310 GAGCCC G[AGT]GC[ACT]^C BpuAI 1540 GAAGAC GAAGAC(2/6) Bsp1286I 310 GAGCCC G[AGT]GC[ACT]^C BspLU11I 2728 ACATGT A^CATGT BsrBI 1588 GAGCGG CCGCTC(-3/-3) BstAPI 2604 GCATATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 991 TTCGAA TT^CGAA DraI 233 TTTAAA TTT^AAA EaeI 352 TGGCCA [CT]^GGCC[GA] Eam1105I 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 3314 GAATTC G^AATTC FspI 2264 TGCGCA TGC^GCA HaeII 718 AGCGCC [GA]GCGC^[CT] MluNI 352 TGGCCA TGG^CCA MscI 352 TGGCCA TGG^CCA NspV 991 TTCGAA TT^CGAA PflMI 2617 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1893 ATTAAT AT^TAAT SfuI 991 TTCGAA TT^CGAA SphI 3355 GCATGC GCATG^C Van91I 2617 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 382 CCAGTGCTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3093 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 1839 GTAGAC GT^[AC][GT]AC 2064 GTCTAC AlwI 2359 GATCC GGATC(4/5) 1668 GGATC AvaI 1596 CCCGAG C^[CT]CG[GA]G 2242 CCCGAG BsaI 1555 GAGACC GGTCTC(1/5) 2335 GGTCTC BsmI 1107 GAATGC GAATGC(1/-1) 2836 GCATTC BsoBI 1596 CCCGAG C^[CT]CG[GA]G 2242 CCCGAG Bsp1407I 1717 TGTACA T^GTACA 2669 TGTACA BsrGI 1717 TGTACA T^GTACA 2669 TGTACA BstXI 1259 CCAGTATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2379 CCAAAGTGCTGG Cac8I 398 GCAAGC GC[ACGT]^[ACGT]GC 3355 GCATGC EcoT14I 274 CCTTGG C^C[AT][AT]GG 3296 CCTTGG FauI 1507 CCCGC CCCGC(4/6) 2365 CCCGC HindIII 147 AAGCTT A^AGCTT 1224 AAGCTT HpaI 2643 GTTAAC GTT^AAC 3278 GTTAAC NspI 2728 ACATGT [GA]CATG^[CT] 3355 GCATGC SapI 189 GAAGAGC GCTCTTC(1/4) 994 GAAGAGC SmlI 2052 CTCAAG C^T[CT][GA]AG 2352 CTCAAG SspBI 1717 TGTACA T^GTACA 2669 TGTACA StyI 274 CCTTGG C^C[AT][AT]GG 3296 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AvaII BbvCI BbvI BfaI BglI BlpI BpmI Bpu1102I BsaJI BsgI BsiYI BslI BsmAI BsmFI BspHI BsrDI BsrI BssKI BstF5I BstNI CelII CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I