##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvrII BamHI
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspMI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FbaI
FseI HapII HgaI Hin1I HpaII
KasI KpnI KspI MaeII MamI
MfeI MflI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 2728 ACATGT A^C[GA][CT]GT
AhdI 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1893 ATTAAT AT^TAAT
AsnI 1893 ATTAAT AT^TAAT
Asp700I 3093 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2264 TGCGCA TGC^GCA
BalI 352 TGGCCA TGG^CCA
BanI 1322 GGTGCC G^G[CT][GA]CC
BanII 310 GAGCCC G[GA]GC[CT]^C
BbsI 1540 GAAGAC GAAGAC(2/6)
BmyI 310 GAGCCC G[AGT]GC[ACT]^C
BpuAI 1540 GAAGAC GAAGAC(2/6)
Bsp1286I 310 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 2728 ACATGT A^CATGT
BsrBI 1588 GAGCGG CCGCTC(-3/-3)
BstAPI 2604 GCATATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 991 TTCGAA TT^CGAA
DraI 233 TTTAAA TTT^AAA
EaeI 352 TGGCCA [CT]^GGCC[GA]
Eam1105I 685 GACAGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3314 GAATTC G^AATTC
FspI 2264 TGCGCA TGC^GCA
HaeII 718 AGCGCC [GA]GCGC^[CT]
MluNI 352 TGGCCA TGG^CCA
MscI 352 TGGCCA TGG^CCA
NspV 991 TTCGAA TT^CGAA
PflMI 2617 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1893 ATTAAT AT^TAAT
SfuI 991 TTCGAA TT^CGAA
SphI 3355 GCATGC GCATG^C
Van91I 2617 CCATGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 382 CCAGTGCTAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3093 GAATCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1839 GTAGAC GT^[AC][GT]AC
2064 GTCTAC
AlwI 2359 GATCC GGATC(4/5)
1668 GGATC
AvaI 1596 CCCGAG C^[CT]CG[GA]G
2242 CCCGAG
BsaI 1555 GAGACC GGTCTC(1/5)
2335 GGTCTC
BsmI 1107 GAATGC GAATGC(1/-1)
2836 GCATTC
BsoBI 1596 CCCGAG C^[CT]CG[GA]G
2242 CCCGAG
Bsp1407I 1717 TGTACA T^GTACA
2669 TGTACA
BsrGI 1717 TGTACA T^GTACA
2669 TGTACA
BstXI 1259 CCAGTATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2379 CCAAAGTGCTGG
Cac8I 398 GCAAGC GC[ACGT]^[ACGT]GC
3355 GCATGC
EcoT14I 274 CCTTGG C^C[AT][AT]GG
3296 CCTTGG
FauI 1507 CCCGC CCCGC(4/6)
2365 CCCGC
HindIII 147 AAGCTT A^AGCTT
1224 AAGCTT
HpaI 2643 GTTAAC GTT^AAC
3278 GTTAAC
NspI 2728 ACATGT [GA]CATG^[CT]
3355 GCATGC
SapI 189 GAAGAGC GCTCTTC(1/4)
994 GAAGAGC
SmlI 2052 CTCAAG C^T[CT][GA]AG
2352 CTCAAG
SspBI 1717 TGTACA T^GTACA
2669 TGTACA
StyI 274 CCTTGG C^C[AT][AT]GG
3296 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvCI BbvI BfaI BglI
BlpI BpmI Bpu1102I BsaJI BsgI
BsiYI BslI BsmAI BsmFI BspHI
BsrDI BsrI BssKI BstF5I BstNI
CelII CfoI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeI NdeII NlaIII
NlaIV PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I