##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI AflII AocI
AscI Asp700I Asp718I AvrII BamHI
BbvCI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsiWI
BspDI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI DraI Eco81I EcoRI
EcoRV FbaI FseI HindIII KpnI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PmeI Psp1406I SacI SalI SapI
SfuI SgrAI SnaBI SpeI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 424 GTCTAC GT^[AC][GT]AC
AccIII 2977 TCCGGA T^CCGGA
AgeI 1659 ACCGGT A^CCGGT
AhdI 2393 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2522 ATTAAT AT^TAAT
AsnI 2522 ATTAAT AT^TAAT
AspEI 2393 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2853 TGCGCA TGC^GCA
BbeI 904 GGCGCC GGCGC^C
BcgI 2560 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 2977 TCCGGA T^CCGGA
BsmI 2168 GAATGC GAATGC(1/-1)
Bsp1407I 673 TGTACA T^GTACA
BspEI 2977 TCCGGA T^CCGGA
BspHI 937 TCATGA T^CATGA
BsrGI 673 TGTACA T^GTACA
BssHII 2770 GCGCGC G^CGCGC
BstEII 513 GGTCACC G^GT[ACGT]ACC
BstPI 513 GGTCACC G^GT[ACGT]ACC
CpoI 2555 CGGACCG CG^G[AT]CCG
DrdI 2150 GACATTTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1683 CGGCCG C^GGCCG
Eam1105I 2393 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1683 CGGCCG C^GGCCG
Eco52I 1683 CGGCCG C^GGCCG
EcoO65I 513 GGTCACC G^GT[ACGT]ACC
EcoT22I 2526 ATGCAT ATGCA^T
FspI 2853 TGCGCA TGC^GCA
HincII 992 GTTAAC GT[CT]^[GA]AC
HindII 992 GTTAAC GT[CT]^[GA]AC
HpaI 992 GTTAAC GTT^AAC
KasI 904 GGCGCC G^GCGCC
MroI 2977 TCCGGA T^CCGGA
NaeI 93 GCCGGC GCC^GGC
NarI 904 GGCGCC GG^CGCC
NgoMIV 93 GCCGGC G^CCGGC
NsiI 2526 ATGCAT ATGCA^T
PaeR7I 973 CTCGAG C^TCGAG
PflMI 3184 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1659 ACCGGT A^CCGGT
PshAI 971 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2522 ATTAAT AT^TAAT
PvuI 834 CGATCG CGAT^CG
RcaI 937 TCATGA T^CATGA
RsrII 2555 CGGACCG CG^G[AT]CCG
SbfI 518 CCTGCAGG CCTGCA^GG
SexAI 2490 ACCAGGT A^CC[AT]GGT
SfoI 904 GGCGCC GGC^GCC
SphI 2921 GCATGC GCATG^C
Sse8387I 518 CCTGCAGG CCTGCA^GG
SspBI 673 TGTACA T^GTACA
TfiI 2320 GATTC G^A[AT]TC
Van91I 3184 CCACCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1474 TCTAGA T^CTAGA
XcmI 1111 CCACAGCGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 973 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1985 GAATTT [GA]^AATT[CT]
2012 AAATTC
Alw44I 3154 GTGCAC G^TGCAC
3409 GTGCAC
AlwI 155 GATCC GGATC(4/5)
2353 GGATC
Aor51HI 850 AGCGCT AGC^GCT
3363 AGCGCT
ApaLI 3154 GTGCAC G^TGCAC
3409 GTGCAC
ApoI 1985 GAATTT [GA]^AATT[CT]
2012 AAATTC
AspI 2189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2489 GACCAGGTC
BbsI 1420 GAAGAC GAAGAC(2/6)
2273 GTCTTC
BpuAI 1420 GAAGAC GAAGAC(2/6)
2273 GTCTTC
BspLU11I 117 ACATGT A^CATGT
665 ACATGT
BspMI 517 ACCTGC ACCTGC(4/8)
2208 ACCTGC
BsrBI 1921 CCGCTC CCGCTC(-3/-3)
2860 CCGCTC
BssSI 1316 CACGAG CACGAG(-5/-1)
3404 CACGAG
BstYI 154 AGATCC [GA]^GATC[CT]
2353 GGATCT
DraIII 1696 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3185 CACCGCGTG
Eco47III 850 AGCGCT AGC^GCT
3363 AGCGCT
EcoNI 475 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2209 CCTGCGGGAGG
MflI 154 AGATCC [GA]^GATC[CT]
2353 GGATCT
MseI 993 TTAA T^TAA
2523 TTAA
PflFI 2189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2489 GACCAGGTC
PpuMI 1383 AGGACCC [GA]G^G[AT]CC[CT]
2426 GGGACCC
ScaI 451 AGTACT AGT^ACT
1893 AGTACT
SfiI 567 GGCCTGCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
816 GGCCAGCCAGGCC
SmaI 23 CCCGGG CCC^GGG
2423 CCCGGG
SmlI 220 CTCAAG C^T[CT][GA]AG
973 CTCGAG
Tru9I 993 TTAA T^TAA
2523 TTAA
Tsp509I 1986 AATT ^AATT
2013 AATT
Tth111I 2189 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2489 GACCAGGTC
XhoII 154 AGATCC [GA]^GATC[CT]
2353 GGATCT
XmaCI 23 CCCGGG C^CCGGG
2423 CCCGGG
XmaI 23 CCCGGG C^CCGGG
2423 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I