##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AvaII AviII AvrII BamHI
BanI BbeI BbrPI BcgI BciVI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT14I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I StyI TaqI
TthHB8I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 3024 CGCG CG^CG
AlwNI 1461 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 781 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1256 CCCGGG C^[CT]CG[GA]G
BalI 488 TGGCCA TGG^CCA
BglI 481 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1924 AGATCT A^GATCT
BsaI 3287 GAGACC GGTCTC(1/5)
BsmI 2682 GCATTC GAATGC(1/-1)
BsoBI 1256 CCCGGG C^[CT]CG[GA]G
BspHI 3681 TCATGA T^CATGA
BspMI 527 ACCTGC ACCTGC(4/8)
BsrDI 122 GCAATG GCAATG(2/0)
BsrFI 2643 GCCGGT [GA]^CCGG[CT]
BstUI 3024 CGCG CG^CG
Cfr10I 2643 GCCGGT [GA]^CCGG[CT]
DraII 999 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 892 CCGTGG C^C[GA][CT]GG
EaeI 488 TGGCCA [CT]^GGCC[GA]
EcoO109I 999 AGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 1680 GTCAAC GT[CT]^[GA]AC
HindII 1680 GTCAAC GT[CT]^[GA]AC
MluNI 488 TGGCCA TGG^CCA
MscI 488 TGGCCA TGG^CCA
MspA1I 755 CCGCTG C[AC]G^C[GT]G
MvnI 3024 CGCG CG^CG
NdeI 1750 CATATG CA^TATG
PflFI 781 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 419 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3681 TCATGA T^CATGA
ScaI 639 AGTACT AGT^ACT
SmaI 1256 CCCGGG CCC^GGG
StuI 1840 AGGCCT AGG^CCT
SwaI 1901 ATTTAAAT ATTT^AAAT
Tth111I 781 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 160 TCTAGA T^CTAGA
XmaCI 1256 CCCGGG C^CCGGG
XmaI 1256 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 3272 CTTAAG C^TTAAG
3583 CTTAAG
AflIII 175 ACATGT A^C[GA][CT]GT
3081 ACATGT
AseI 603 ATTAAT AT^TAAT
3088 ATTAAT
AsnI 603 ATTAAT AT^TAAT
3088 ATTAAT
BbvCI 397 CCTCAGC CCTCAGC(-5/-2)
1279 CCTCAGC
BclI 1661 TGATCA T^GATCA
2296 TGATCA
BfrI 3272 CTTAAG C^TTAAG
3583 CTTAAG
BsaWI 2139 ACCGGA [AT]^CCGG[AT]
3608 ACCGGA
BspLU11I 175 ACATGT A^CATGT
3081 ACATGT
Cac8I 942 GCATGC GC[ACGT]^[ACGT]GC
3578 GCATGC
Cfr13I 535 GGGCC G^G[ACGT]CC
1000 GGCCC
DraI 59 TTTAAA TTT^AAA
1902 TTTAAA
FauI 1275 CCCGC CCCGC(4/6)
1601 CCCGC
FbaI 1661 TGATCA T^GATCA
2296 TGATCA
PleI 1455 GACTC GAGTC(4/5)
206 GAGTC
PshBI 603 ATTAAT AT^TAAT
3088 ATTAAT
PstI 421 CTGCAG CTGCA^G
1623 CTGCAG
SacI 30 GAGCTC GAGCT^C
504 GAGCTC
Sau96I 535 GGGCC G^G[ACGT]CC
1000 GGCCC
SphI 942 GCATGC GCATG^C
3578 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI DdeI DpnII EarI
EcoRI EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NsiI NspI
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI SspBI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI