##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
AocI AscI AspEI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DsaI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI KasI KspI
MfeI MflI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 2388 GGCGTC G[GA]^CG[CT]C
AflII 3037 CTTAAG C^TTAAG
AflIII 763 ACATGT A^C[GA][CT]GT
Alw44I 2117 GTGCAC G^TGCAC
AlwNI 1965 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2790 AGCGCT AGC^GCT
ApaI 63 GGGCCC GGGCC^C
ApaLI 2117 GTGCAC G^TGCAC
AseI 34 ATTAAT AT^TAAT
AsnI 34 ATTAAT AT^TAAT
AspI 408 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 2759 GAAGAC GAAGAC(2/6)
BfrI 3037 CTTAAG C^TTAAG
BlpI 2786 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2786 GCTAAGC GC^T[ACGT]AGC
BpuAI 2759 GAAGAC GAAGAC(2/6)
BsaAI 3087 TACGTG [CT]AC^GT[GA]
BsaHI 2388 GGCGTC G[GA]^CG[CT]C
BsiEI 92 CGGCCG CG[GA][CT]^CG
BsmI 1615 GAATGC GAATGC(1/-1)
BspLU11I 763 ACATGT A^CATGT
BsrBI 129 GAGCGG CCGCTC(-3/-3)
BstXI 243 CCAAGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2786 GCTAAGC GC^T[ACGT]AGC
DrdI 968 GACTTCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 92 CGGCCG [CT]^GGCC[GA]
EagI 92 CGGCCG C^GGCCG
EclXI 92 CGGCCG C^GGCCG
Eco47III 2790 AGCGCT AGC^GCT
Eco52I 92 CGGCCG C^GGCCG
EcoRI 1473 GAATTC G^AATTC
Hin1I 2388 GGCGTC G[GA]^CG[CT]C
HpaI 1450 GTTAAC GTT^AAC
PflFI 408 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2672 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 34 ATTAAT AT^TAAT
ScaI 1648 AGTACT AGT^ACT
SspI 811 AATATT AAT^ATT
Tth111I 408 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 1531 GGTACC G^GTACC
2146 GGTACC
Asp718I 1531 GGTACC G^GTACC
2146 GGTACC
AvaI 120 CCCGAG C^[CT]CG[GA]G
394 CTCGGG
BanI 1531 GGTACC G^G[CT][GA]CC
2146 GGTACC
BcnI 66 CCCGG CC^[GC]GG
95 CCGGG
BsaBI 828 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2483 GATAACAATC
BsgI 1822 CTGCAC GTGCAG(16/14)
2004 CTGCAC
BsoBI 120 CCCGAG C^[CT]CG[GA]G
394 CTCGGG
Bsp1407I 3015 TGTACA T^GTACA
3146 TGTACA
BsrDI 1592 CATTGC GCAATG(2/0)
3315 GCAATG
BsrGI 3015 TGTACA T^GTACA
3146 TGTACA
EcoT14I 444 CCAAGG C^C[AT][AT]GG
3358 CCTTGG
HaeII 530 AGCGCC [GA]GCGC^[CT]
2790 AGCGCT
HapII 67 CCGG C^CGG
95 CCGG
HincII 1069 GTCAAC GT[CT]^[GA]AC
1450 GTTAAC
HindII 1069 GTCAAC GT[CT]^[GA]AC
1450 GTTAAC
HindIII 1886 AAGCTT A^AGCTT
2504 AAGCTT
HpaII 67 CCGG C^CGG
95 CCGG
KpnI 1531 GGTACC GGTAC^C
2146 GGTACC
MamI 828 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2483 GATAACAATC
MspI 67 CCGG C^CGG
95 CCGG
NciI 66 CCCGG CC^[GC]GG
95 CCGGG
PflMI 425 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3432 CCATTGGTTGG
PpuMI 1018 GGGTCCT [GA]G^G[AT]CC[CT]
2161 GGGTCCC
PvuII 1955 CAGCTG CAG^CTG
3225 CAGCTG
SacI 547 GAGCTC GAGCT^C
1813 GAGCTC
SspBI 3015 TGTACA T^GTACA
3146 TGTACA
StuI 605 AGGCCT AGG^CCT
1872 AGGCCT
StyI 444 CCAAGG C^C[AT][AT]GG
3358 CCTTGG
Van91I 425 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3432 CCATTGGTTGG
XcmI 243 CCAAGAACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2419 CCAAGAGAATGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AclI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI Psp1406I
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI