##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspI AviII AvrII
BanII BbrPI BcgI BclI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I Cfr10I
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII KspI MaeII MamI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccII 1417 CGCG CG^CG
AflIII 3219 ACATGT A^C[GA][CT]GT
AhdI 255 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 255 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2826 TGGCCA TGG^CCA
BamHI 2865 GGATCC G^GATCC
BbsI 244 GAAGAC GAAGAC(2/6)
BbvCI 710 CCTCAGC CCTCAGC(-5/-2)
BglII 616 AGATCT A^GATCT
BlpI 2291 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2291 GCTGAGC GC^T[ACGT]AGC
BpuAI 244 GAAGAC GAAGAC(2/6)
BsmI 3185 GCATTC GAATGC(1/-1)
BspDI 2488 ATCGAT AT^CGAT
BspLU11I 3219 ACATGT A^CATGT
Bst1107I 2501 GTATAC GTA^TAC
BstUI 1417 CGCG CG^CG
BstZ17I 2501 GTATAC GTA^TAC
CelII 2291 GCTGAGC GC^T[ACGT]AGC
ClaI 2488 ATCGAT AT^CGAT
DraII 3148 AGGACCC [GA]G^G[ACGT]CC[CT]
Eam1105I 255 GACAGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 3148 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1643 GAATTC G^AATTC
HpaI 3127 GTTAAC GTT^AAC
MfeI 1900 CAATTG C^AATTG
MluNI 2826 TGGCCA TGG^CCA
MscI 2826 TGGCCA TGG^CCA
MunI 1900 CAATTG C^AATTG
MvnI 1417 CGCG CG^CG
PpuMI 3148 AGGACCC [GA]G^G[AT]CC[CT]
SapI 1591 GCTCTTC GCTCTTC(1/4)
SmaI 3243 CCCGGG CCC^GGG
StuI 766 AGGCCT AGG^CCT
SwaI 2875 ATTTAAAT ATTT^AAAT
XmaCI 3243 CCCGGG C^CCGGG
XmaI 3243 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 565 GGTACC G^GTACC
1390 GGTACC
AcyI 14 GGCGCC G[GA]^CG[CT]C
100 GGCGCC
AlwNI 731 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2471 CAGGATCTG
AseI 2061 ATTAAT AT^TAAT
2417 ATTAAT
AsnI 2061 ATTAAT AT^TAAT
2417 ATTAAT
Asp718I 565 GGTACC G^GTACC
1390 GGTACC
AspHI 808 GAGCAC G[AT]GC[AT]^C
893 GTGCTC
BbeI 14 GGCGCC GGCGC^C
100 GGCGCC
BciVI 609 GGATAC GTATCC(6/5)
1327 GGATAC
BpmI 728 CTCCAG CTGGAG(16/14)
887 CTCCAG
BsaHI 14 GGCGCC G[GA]^CG[CT]C
100 GGCGCC
BsaI 1248 GAGACC GGTCTC(1/5)
2314 GGTCTC
BseRI 1340 GAGGAG GAGGAG(10/8)
1430 GAGGAG
BsgI 2956 CTGCAC GTGCAG(16/14)
700 GTGCAG
BsiHKAI 808 GAGCAC G[AT]GC[AT]^C
893 GTGCTC
BsmFI 1835 GGGAC GGGAC(10/14)
3241 GTCCC
Bsp1407I 1651 TGTACA T^GTACA
2105 TGTACA
BspHI 2634 TCATGA T^CATGA
3160 TCATGA
BspMI 723 ACCTGC ACCTGC(4/8)
3380 ACCTGC
BsrDI 2177 CATTGC GCAATG(2/0)
2764 CATTGC
BsrGI 1651 TGTACA T^GTACA
2105 TGTACA
CfoI 15 GCGC GCG^C
101 GCGC
EaeI 1317 TGGCCG [CT]^GGCC[GA]
2826 TGGCCA
EarI 1592 CTCTTC CTCTTC(1/4)
1027 GAAGAG
EcoNI 2569 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3140 CCTTTTAGAGG
HaeII 14 GGCGCC [GA]GCGC^[CT]
100 GGCGCC
HapII 1446 CCGG C^CGG
3244 CCGG
HhaI 15 GCGC GCG^C
101 GCGC
Hin1I 14 GGCGCC G[GA]^CG[CT]C
100 GGCGCC
HinP1I 15 GCGC G^CGC
101 GCGC
HpaII 1446 CCGG C^CGG
3244 CCGG
KasI 14 GGCGCC G^GCGCC
100 GGCGCC
KpnI 565 GGTACC GGTAC^C
1390 GGTACC
Ksp632I 1592 CTCTTC CTCTTC(1/4)
1027 GAAGAG
MslI 928 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2830 CACTTTTGTG
MspA1I 80 CAGCTG C[AC]G^C[GT]G
734 CAGCTG
MspI 1446 CCGG C^CGG
3244 CCGG
NarI 14 GGCGCC GG^CGCC
100 GGCGCC
NspI 3123 GCATGT [GA]CATG^[CT]
3219 ACATGT
PaeR7I 275 CTCGAG C^TCGAG
1406 CTCGAG
PshBI 2061 ATTAAT AT^TAAT
2417 ATTAAT
PstI 1914 CTGCAG CTGCA^G
3382 CTGCAG
PvuII 80 CAGCTG CAG^CTG
734 CAGCTG
RcaI 2634 TCATGA T^CATGA
3160 TCATGA
ScaI 1824 AGTACT AGT^ACT
3074 AGTACT
SfoI 14 GGCGCC GGC^GCC
100 GGCGCC
SpeI 2782 ACTAGT A^CTAGT
3276 ACTAGT
SspBI 1651 TGTACA T^GTACA
2105 TGTACA
Tsp45I 314 GTGAC ^GT[GC]AC
1660 GTCAC
XhoI 275 CTCGAG C^TCGAG
1406 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanI
BbvI BcnI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
Van91I XhoII