##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
Acc65I AccII AclI AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BglI BlnI BlpI Bpu1102I BsaBI
BsaJI BsgI BsiEI BsiWI BsmBI
BsoBI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI CelII CfoI Cfr10I CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI HinP1I HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StyI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 607 GACGTC GACGT^C
AccIII 661 TCCGGA T^CCGGA
AcyI 607 GACGTC G[GA]^CG[CT]C
AocI 737 CCTTAGG CC^T[ACGT]AGG
BanII 3462 GAGCTC G[GA]GC[CT]^C
BbsI 217 GAAGAC GAAGAC(2/6)
BbvCI 548 CCTCAGC CCTCAGC(-5/-2)
BpuAI 217 GAAGAC GAAGAC(2/6)
BsaAI 1060 TACGTG [CT]AC^GT[GA]
BsaHI 607 GACGTC G[GA]^CG[CT]C
BseAI 661 TCCGGA T^CCGGA
BsmFI 187 GGGAC GGGAC(10/14)
Bsp1407I 2016 TGTACA T^GTACA
BspDI 451 ATCGAT AT^CGAT
BspEI 661 TCCGGA T^CCGGA
BspHI 1963 TCATGA T^CATGA
BspLU11I 241 ACATGT A^CATGT
BspMI 3041 GCAGGT ACCTGC(4/8)
BsrGI 2016 TGTACA T^GTACA
Bst1107I 3005 GTATAC GTA^TAC
BstZ17I 3005 GTATAC GTA^TAC
Bsu36I 737 CCTTAGG CC^T[ACGT]AGG
ClaI 451 ATCGAT AT^CGAT
Eco81I 737 CCTTAGG CC^T[ACGT]AGG
EcoNI 2240 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 553 GCGGG CCCGC(4/6)
Hin1I 607 GACGTC G[GA]^CG[CT]C
HincII 3701 GTCAAC GT[CT]^[GA]AC
HindII 3701 GTCAAC GT[CT]^[GA]AC
HindIII 3275 AAGCTT A^AGCTT
MroI 661 TCCGGA T^CCGGA
NdeI 472 CATATG CA^TATG
RcaI 1963 TCATGA T^CATGA
SacI 3462 GAGCTC GAGCT^C
SapI 3464 GCTCTTC GCTCTTC(1/4)
SexAI 1880 ACCAGGT A^CC[AT]GGT
SspBI 2016 TGTACA T^GTACA
StuI 2905 AGGCCT AGG^CCT
XbaI 1631 TCTAGA T^CTAGA
XcmI 2059 CCATGTAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2836 GTCTAC GT^[AC][GT]AC
3005 GTATAC
AflII 538 CTTAAG C^TTAAG
1992 CTTAAG
AflIII 241 ACATGT A^C[GA][CT]GT
1061 ACGTGT
AlwI 319 GATCC GGATC(4/5)
1360 GATCC
BalI 1084 TGGCCA TGG^CCA
3399 TGGCCA
BanI 1024 GGTGCC G^G[CT][GA]CC
2922 GGCACC
BfrI 538 CTTAAG C^TTAAG
1992 CTTAAG
BglII 1329 AGATCT A^GATCT
2738 AGATCT
BsaI 287 GGTCTC GGTCTC(1/5)
701 GGTCTC
BsaWI 661 TCCGGA [AT]^CCGG[AT]
1129 TCCGGT
BseRI 2487 CTCCTC GAGGAG(10/8)
1997 GAGGAG
BsmI 711 GAATGC GAATGC(1/-1)
1350 GAATGC
Cac8I 424 GCGAGC GC[ACGT]^[ACGT]GC
1086 GCCAGC
DraIII 460 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
1912 CACTTGGTG
EaeI 1084 TGGCCA [CT]^GGCC[GA]
3399 TGGCCA
MluNI 1084 TGGCCA TGG^CCA
3399 TGGCCA
MscI 1084 TGGCCA TGG^CCA
3399 TGGCCA
PpuMI 1337 AGGACCT [GA]G^G[AT]CC[CT]
2236 GGGTCCT
PstI 1408 CTGCAG CTGCA^G
2915 CTGCAG
PvuII 706 CAGCTG CAG^CTG
2220 CAGCTG
ScaI 1488 AGTACT AGT^ACT
3600 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AseI AsnI AspHI AvaII BbvI
BfaI BmyI BpmI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII