################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## Acc65I AccII AclI AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaBI BsaJI BsgI BsiEI BsiWI BsmBI BsoBI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI CelII CfoI Cfr10I CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 607 GACGTC GACGT^C AccIII 661 TCCGGA T^CCGGA AcyI 607 GACGTC G[GA]^CG[CT]C AocI 737 CCTTAGG CC^T[ACGT]AGG BanII 3462 GAGCTC G[GA]GC[CT]^C BbsI 217 GAAGAC GAAGAC(2/6) BbvCI 548 CCTCAGC CCTCAGC(-5/-2) BpuAI 217 GAAGAC GAAGAC(2/6) BsaAI 1060 TACGTG [CT]AC^GT[GA] BsaHI 607 GACGTC G[GA]^CG[CT]C BseAI 661 TCCGGA T^CCGGA BsmFI 187 GGGAC GGGAC(10/14) Bsp1407I 2016 TGTACA T^GTACA BspDI 451 ATCGAT AT^CGAT BspEI 661 TCCGGA T^CCGGA BspHI 1963 TCATGA T^CATGA BspLU11I 241 ACATGT A^CATGT BspMI 3041 GCAGGT ACCTGC(4/8) BsrGI 2016 TGTACA T^GTACA Bst1107I 3005 GTATAC GTA^TAC BstZ17I 3005 GTATAC GTA^TAC Bsu36I 737 CCTTAGG CC^T[ACGT]AGG ClaI 451 ATCGAT AT^CGAT Eco81I 737 CCTTAGG CC^T[ACGT]AGG EcoNI 2240 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 553 GCGGG CCCGC(4/6) Hin1I 607 GACGTC G[GA]^CG[CT]C HincII 3701 GTCAAC GT[CT]^[GA]AC HindII 3701 GTCAAC GT[CT]^[GA]AC HindIII 3275 AAGCTT A^AGCTT MroI 661 TCCGGA T^CCGGA NdeI 472 CATATG CA^TATG RcaI 1963 TCATGA T^CATGA SacI 3462 GAGCTC GAGCT^C SapI 3464 GCTCTTC GCTCTTC(1/4) SexAI 1880 ACCAGGT A^CC[AT]GGT SspBI 2016 TGTACA T^GTACA StuI 2905 AGGCCT AGG^CCT XbaI 1631 TCTAGA T^CTAGA XcmI 2059 CCATGTAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 2836 GTCTAC GT^[AC][GT]AC 3005 GTATAC AflII 538 CTTAAG C^TTAAG 1992 CTTAAG AflIII 241 ACATGT A^C[GA][CT]GT 1061 ACGTGT AlwI 319 GATCC GGATC(4/5) 1360 GATCC BalI 1084 TGGCCA TGG^CCA 3399 TGGCCA BanI 1024 GGTGCC G^G[CT][GA]CC 2922 GGCACC BfrI 538 CTTAAG C^TTAAG 1992 CTTAAG BglII 1329 AGATCT A^GATCT 2738 AGATCT BsaI 287 GGTCTC GGTCTC(1/5) 701 GGTCTC BsaWI 661 TCCGGA [AT]^CCGG[AT] 1129 TCCGGT BseRI 2487 CTCCTC GAGGAG(10/8) 1997 GAGGAG BsmI 711 GAATGC GAATGC(1/-1) 1350 GAATGC Cac8I 424 GCGAGC GC[ACGT]^[ACGT]GC 1086 GCCAGC DraIII 460 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG 1912 CACTTGGTG EaeI 1084 TGGCCA [CT]^GGCC[GA] 3399 TGGCCA MluNI 1084 TGGCCA TGG^CCA 3399 TGGCCA MscI 1084 TGGCCA TGG^CCA 3399 TGGCCA PpuMI 1337 AGGACCT [GA]G^G[AT]CC[CT] 2236 GGGTCCT PstI 1408 CTGCAG CTGCA^G 2915 CTGCAG PvuII 706 CAGCTG CAG^CTG 2220 CAGCTG ScaI 1488 AGTACT AGT^ACT 3600 AGTACT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AseI AsnI AspHI AvaII BbvI BfaI BmyI BpmI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII