##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BcgI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
ClaI DraI DrdI Eam1105I Eco47III
Eco81I EcoT22I FauI FseI FspI
HincII HindII HpaI KpnI KspI
MamI MluI MroI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI SacII SalI ScaI
SfiI SfuI SmlI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AgeI 176 ACCGGT A^CCGGT
AvaI 918 CCCGGG C^[CT]CG[GA]G
BbeI 2084 GGCGCC GGCGC^C
BbvCI 833 GCTGAGG CCTCAGC(-5/-2)
BciVI 683 GGATAC GTATCC(6/5)
BclI 2420 TGATCA T^GATCA
BglI 1428 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2216 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2216 GCTGAGC GC^T[ACGT]AGC
BsaI 1854 GAGACC GGTCTC(1/5)
BsaWI 176 ACCGGT [AT]^CCGG[AT]
BsiEI 2379 CGGCCG CG[GA][CT]^CG
BsmBI 812 CGTCTC CGTCTC(1/5)
BsoBI 918 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1197 TGTACA T^GTACA
BsrFI 176 ACCGGT [GA]^CCGG[CT]
BsrGI 1197 TGTACA T^GTACA
BstYI 2142 GGATCT [GA]^GATC[CT]
CelII 2216 GCTGAGC GC^T[ACGT]AGC
Cfr10I 176 ACCGGT [GA]^CCGG[CT]
CpoI 2366 CGGTCCG CG^G[AT]CCG
DraIII 1247 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2379 CGGCCG C^GGCCG
EclXI 2379 CGGCCG C^GGCCG
Eco52I 2379 CGGCCG C^GGCCG
EcoRI 975 GAATTC G^AATTC
EcoRV 2645 GATATC GAT^ATC
FbaI 2420 TGATCA T^GATCA
HaeII 2084 GGCGCC [GA]GCGC^[CT]
KasI 2084 GGCGCC G^GCGCC
MaeII 1266 ACGT A^CGT
MflI 2142 GGATCT [GA]^GATC[CT]
NarI 2084 GGCGCC GG^CGCC
PinAI 176 ACCGGT A^CCGGT
RsrII 2366 CGGTCCG CG^G[AT]CCG
SbfI 1858 CCTGCAGG CCTGCA^GG
SexAI 357 ACCTGGT A^CC[AT]GGT
SfoI 2084 GGCGCC GGC^GCC
SgrAI 175 CACCGGTG C[GA]^CCGG[CT]G
SmaI 918 CCCGGG CCC^GGG
Sse8387I 1858 CCTGCAGG CCTGCA^GG
SspBI 1197 TGTACA T^GTACA
StuI 214 AGGCCT AGG^CCT
XhoII 2142 GGATCT [GA]^GATC[CT]
XmaCI 918 CCCGGG C^CCGGG
XmaI 918 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 955 GACGCC G[GA]^CG[CT]C
2084 GGCGCC
BsaHI 955 GACGCC G[GA]^CG[CT]C
2084 GGCGCC
BsgI 1693 CTGCAC GTGCAG(16/14)
941 GTGCAG
BsmI 136 GAATGC GAATGC(1/-1)
1291 GAATGC
BspHI 45 TCATGA T^CATGA
1179 TCATGA
BspMI 1857 ACCTGC ACCTGC(4/8)
470 GCAGGT
BsrDI 97 CATTGC GCAATG(2/0)
1938 GCAATG
BstEII 1064 GGTAACC G^GT[ACGT]ACC
1105 GGTGACC
BstPI 1064 GGTAACC G^GT[ACGT]ACC
1105 GGTGACC
CfoI 1078 GCGC GCG^C
2085 GCGC
EarI 56 GAAGAG CTCTTC(1/4)
1471 GAAGAG
EcoNI 1324 CCTTATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1372 CCTGGCCAAGG
EcoO65I 1064 GGTAACC G^GT[ACGT]ACC
1105 GGTGACC
HhaI 1078 GCGC GCG^C
2085 GCGC
Hin1I 955 GACGCC G[GA]^CG[CT]C
2084 GGCGCC
HinP1I 1078 GCGC G^CGC
2085 GCGC
HindIII 1202 AAGCTT A^AGCTT
2680 AAGCTT
Ksp632I 56 GAAGAG CTCTTC(1/4)
1471 GAAGAG
MfeI 537 CAATTG C^AATTG
843 CAATTG
MunI 537 CAATTG C^AATTG
843 CAATTG
NcoI 1128 CCATGG C^CATGG
1791 CCATGG
NspI 937 GCATGT [GA]CATG^[CT]
2028 ACATGC
PflMI 2206 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGGAGCTGG
PpuMI 797 AGGACCC [GA]G^G[AT]CC[CT]
1025 GGGACCC
PstI 1281 CTGCAG CTGCA^G
1859 CTGCAG
RcaI 45 TCATGA T^CATGA
1179 TCATGA
SacI 1595 GAGCTC GAGCT^C
2560 GAGCTC
SapI 56 GAAGAGC GCTCTTC(1/4)
1471 GAAGAGC
Van91I 2206 CCAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2530 CCAGGAGCTGG
XcmI 749 CCAGATGACGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1791 CCATGGCTGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I