################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AccII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII BbeI BbvCI BcgI BciVI BfrI BlpI Bpu1102I BseAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII Bst1107I BstUI BstZ17I CelII ClaI CpoI DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HincII HindII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 844 GACGTC GACGT^C Acc65I 1080 GGTACC G^GTACC AccI 2972 GTCTAC GT^[AC][GT]AC AflIII 1092 ACGTGT A^C[GA][CT]GT AocI 11 CCTGAGG CC^T[ACGT]AGG Asp718I 1080 GGTACC G^GTACC BamHI 2076 GGATCC G^GATCC BbrPI 1091 CACGTG CAC^GTG BclI 90 TGATCA T^GATCA BsaAI 1091 CACGTG [CT]AC^GT[GA] BsaBI 1482 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1332 TCCGGT [AT]^CCGG[AT] BsgI 1373 CTGCAC GTGCAG(16/14) BsiEI 249 CGGCCG CG[GA][CT]^CG Bsp1407I 1095 TGTACA T^GTACA BsrDI 828 CATTGC GCAATG(2/0) BsrFI 192 ACCGGC [GA]^CCGG[CT] BsrGI 1095 TGTACA T^GTACA BssSI 258 CACGAG CACGAG(-5/-1) BstBI 722 TTCGAA TT^CGAA BstEII 1720 GGTGACC G^GT[ACGT]ACC BstPI 1720 GGTGACC G^GT[ACGT]ACC BstXI 2187 CCACTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 11 CCTGAGG CC^T[ACGT]AGG CfoI 860 GCGC GCG^C Cfr10I 192 ACCGGC [GA]^CCGG[CT] DraII 356 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 738 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 249 CGGCCG C^GGCCG EclXI 249 CGGCCG C^GGCCG Eco52I 249 CGGCCG C^GGCCG Eco81I 11 CCTGAGG CC^T[ACGT]AGG EcoNI 1622 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 356 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 1720 GGTGACC G^GT[ACGT]ACC FbaI 90 TGATCA T^GATCA HhaI 860 GCGC GCG^C HinP1I 860 GCGC G^CGC KpnI 1080 GGTACC GGTAC^C MamI 1482 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NspV 722 TTCGAA TT^CGAA PmaCI 1091 CACGTG CAC^GTG PmlI 1091 CACGTG CAC^GTG PpuMI 356 GGGACCT [GA]G^G[AT]CC[CT] PvuII 1370 CAGCTG CAG^CTG SacI 261 GAGCTC GAGCT^C SfuI 722 TTCGAA TT^CGAA SmlI 2867 CTTGAG C^T[CT][GA]AG SphI 1856 GCATGC GCATG^C SspBI 1095 TGTACA T^GTACA XbaI 2649 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 844 GACGTC G[GA]^CG[CT]C 1045 GGCGTC AlwI 2077 GATCC GGATC(4/5) 2076 GGATC AvrII 1943 CCTAGG C^CTAGG 2729 CCTAGG BanI 298 GGCACC G^G[CT][GA]CC 1080 GGTACC BanII 261 GAGCTC G[GA]GC[CT]^C 2349 GGGCTC BcnI 247 CCCGG CC^[GC]GG 630 CCGGG BglI 1037 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2756 GCCTGTTAGGC BglII 536 AGATCT A^GATCT 2283 AGATCT BlnI 1943 CCTAGG C^CTAGG 2729 CCTAGG BsaHI 844 GACGTC G[GA]^CG[CT]C 1045 GGCGTC BsmI 1431 GAATGC GAATGC(1/-1) 2322 GCATTC BstAPI 201 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2017 GCAAAGACTGC Hin1I 844 GACGTC G[GA]^CG[CT]C 1045 GGCGTC HindIII 717 AAGCTT A^AGCTT 3304 AAGCTT MspA1I 27 CAGCGG C[AC]G^C[GT]G 1370 CAGCTG NciI 247 CCCGG CC^[GC]GG 630 CCGGG NcoI 990 CCATGG C^CATGG 2896 CCATGG PshAI 1915 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2696 GACAGTTGTC SexAI 1784 ACCTGGT A^CC[AT]GGT 2088 ACCTGGT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI AvaII BalI BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII