##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII BbeI BbvCI
BcgI BciVI BfrI BlpI Bpu1102I
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BssHII Bst1107I BstUI BstZ17I CelII
ClaI CpoI DrdI Eam1105I Eco47III
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 844 GACGTC GACGT^C
Acc65I 1080 GGTACC G^GTACC
AccI 2972 GTCTAC GT^[AC][GT]AC
AflIII 1092 ACGTGT A^C[GA][CT]GT
AocI 11 CCTGAGG CC^T[ACGT]AGG
Asp718I 1080 GGTACC G^GTACC
BamHI 2076 GGATCC G^GATCC
BbrPI 1091 CACGTG CAC^GTG
BclI 90 TGATCA T^GATCA
BsaAI 1091 CACGTG [CT]AC^GT[GA]
BsaBI 1482 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1332 TCCGGT [AT]^CCGG[AT]
BsgI 1373 CTGCAC GTGCAG(16/14)
BsiEI 249 CGGCCG CG[GA][CT]^CG
Bsp1407I 1095 TGTACA T^GTACA
BsrDI 828 CATTGC GCAATG(2/0)
BsrFI 192 ACCGGC [GA]^CCGG[CT]
BsrGI 1095 TGTACA T^GTACA
BssSI 258 CACGAG CACGAG(-5/-1)
BstBI 722 TTCGAA TT^CGAA
BstEII 1720 GGTGACC G^GT[ACGT]ACC
BstPI 1720 GGTGACC G^GT[ACGT]ACC
BstXI 2187 CCACTGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 11 CCTGAGG CC^T[ACGT]AGG
CfoI 860 GCGC GCG^C
Cfr10I 192 ACCGGC [GA]^CCGG[CT]
DraII 356 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 738 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 249 CGGCCG C^GGCCG
EclXI 249 CGGCCG C^GGCCG
Eco52I 249 CGGCCG C^GGCCG
Eco81I 11 CCTGAGG CC^T[ACGT]AGG
EcoNI 1622 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 356 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1720 GGTGACC G^GT[ACGT]ACC
FbaI 90 TGATCA T^GATCA
HhaI 860 GCGC GCG^C
HinP1I 860 GCGC G^CGC
KpnI 1080 GGTACC GGTAC^C
MamI 1482 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspV 722 TTCGAA TT^CGAA
PmaCI 1091 CACGTG CAC^GTG
PmlI 1091 CACGTG CAC^GTG
PpuMI 356 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 1370 CAGCTG CAG^CTG
SacI 261 GAGCTC GAGCT^C
SfuI 722 TTCGAA TT^CGAA
SmlI 2867 CTTGAG C^T[CT][GA]AG
SphI 1856 GCATGC GCATG^C
SspBI 1095 TGTACA T^GTACA
XbaI 2649 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 844 GACGTC G[GA]^CG[CT]C
1045 GGCGTC
AlwI 2077 GATCC GGATC(4/5)
2076 GGATC
AvrII 1943 CCTAGG C^CTAGG
2729 CCTAGG
BanI 298 GGCACC G^G[CT][GA]CC
1080 GGTACC
BanII 261 GAGCTC G[GA]GC[CT]^C
2349 GGGCTC
BcnI 247 CCCGG CC^[GC]GG
630 CCGGG
BglI 1037 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2756 GCCTGTTAGGC
BglII 536 AGATCT A^GATCT
2283 AGATCT
BlnI 1943 CCTAGG C^CTAGG
2729 CCTAGG
BsaHI 844 GACGTC G[GA]^CG[CT]C
1045 GGCGTC
BsmI 1431 GAATGC GAATGC(1/-1)
2322 GCATTC
BstAPI 201 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2017 GCAAAGACTGC
Hin1I 844 GACGTC G[GA]^CG[CT]C
1045 GGCGTC
HindIII 717 AAGCTT A^AGCTT
3304 AAGCTT
MspA1I 27 CAGCGG C[AC]G^C[GT]G
1370 CAGCTG
NciI 247 CCCGG CC^[GC]GG
630 CCGGG
NcoI 990 CCATGG C^CATGG
2896 CCATGG
PshAI 1915 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2696 GACAGTTGTC
SexAI 1784 ACCTGGT A^CC[AT]GGT
2088 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaII BalI BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII