##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AvaI AviII BanI BbeI BbrPI
BbvCI BcgI BfrI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoT22I
FseI FspI HaeII HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI TaqI
TthHB8I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1088 GACGTC GACGT^C
AccII 38 CGCG CG^CG
AcyI 1088 GACGTC G[GA]^CG[CT]C
AocI 2863 CCTGAGG CC^T[ACGT]AGG
AvrII 2070 CCTAGG C^CTAGG
BalI 640 TGGCCA TGG^CCA
BamHI 2811 GGATCC G^GATCC
BciVI 798 GGATAC GTATCC(6/5)
BclI 1506 TGATCA T^GATCA
BcnI 2189 CCGGG CC^[GC]GG
BglI 2389 GCCTGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2070 CCTAGG C^CTAGG
BsaBI 887 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1088 GACGTC G[GA]^CG[CT]C
BsmI 2909 GAATGC GAATGC(1/-1)
Bsp1407I 96 TGTACA T^GTACA
BsrFI 1802 ACCGGC [GA]^CCGG[CT]
BsrGI 96 TGTACA T^GTACA
BstUI 38 CGCG CG^CG
Bsu36I 2863 CCTGAGG CC^T[ACGT]AGG
CfoI 37 GCGC GCG^C
Cfr10I 1802 ACCGGC [GA]^CCGG[CT]
DsaI 438 CCACGG C^C[GA][CT]GG
EarI 2896 GAAGAG CTCTTC(1/4)
Eco81I 2863 CCTGAGG CC^T[ACGT]AGG
FauI 1674 GCGGG CCCGC(4/6)
FbaI 1506 TGATCA T^GATCA
HhaI 37 GCGC GCG^C
Hin1I 1088 GACGTC G[GA]^CG[CT]C
HinP1I 37 GCGC G^CGC
Ksp632I 2896 GAAGAG CTCTTC(1/4)
MamI 887 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 640 TGGCCA TGG^CCA
MscI 640 TGGCCA TGG^CCA
MvnI 38 CGCG CG^CG
NciI 2189 CCGGG CC^[GC]GG
PflMI 1718 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2525 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2040 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspBI 96 TGTACA T^GTACA
SspI 1377 AATATT AAT^ATT
Van91I 1718 CCAGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1961 CCATCTCACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Asp700I 1297 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2663 GAACCATTTC
AspI 2457 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2977 GACTCTGTC
AvaII 929 GGACC G^G[AT]CC
2526 GGACC
BanII 443 GAGCTC G[GA]GC[CT]^C
2960 GAGCTC
BglII 363 AGATCT A^GATCT
1837 AGATCT
BsgI 2429 CTGCAC GTGCAG(16/14)
29 GTGCAG
BstAPI 311 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2916 GCATTACCTGC
DraIII 1499 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1761 CACTGTGTG
EcoNI 2517 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3140 CCTAGTTTAGG
EcoRI 86 GAATTC G^AATTC
2141 GAATTC
EcoRV 47 GATATC GAT^ATC
1245 GATATC
HapII 1803 CCGG C^CGG
2189 CCGG
HgaI 609 GACGC GACGC(5/10)
1195 GCGTC
HindIII 374 AAGCTT A^AGCTT
1770 AAGCTT
HpaII 1803 CCGG C^CGG
2189 CCGG
MslI 1758 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2120 CATTCTTGTG
MspI 1803 CCGG C^CGG
2189 CCGG
PflFI 2457 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2977 GACTCTGTC
PvuII 5 CAGCTG CAG^CTG
2352 CAGCTG
SacI 443 GAGCTC GAGCT^C
2960 GAGCTC
ScaI 877 AGTACT AGT^ACT
2496 AGTACT
TfiI 148 GAATC G^A[AT]TC
1706 GAATC
Tth111I 2457 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2977 GACTCTGTC
XmnI 1297 GAAACGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2663 GAACCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII