################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BseAI BsiEI BsmBI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstXI BstYI CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccII 2167 CGCG CG^CG AflII 3363 CTTAAG C^TTAAG AflIII 705 ACATGT A^C[GA][CT]GT AspHI 3098 GAGCTC G[AT]GC[AT]^C AviII 2345 TGCGCA TGC^GCA BciVI 2963 GTATCC GTATCC(6/5) BcnI 1525 CCGGG CC^[GC]GG BfrI 3363 CTTAAG C^TTAAG BsaBI 1419 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 2050 GTGCAG GTGCAG(16/14) BsiHKAI 3098 GAGCTC G[AT]GC[AT]^C BsiWI 2293 CGTACG C^GTACG BspLU11I 705 ACATGT A^CATGT BstAPI 2218 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 2167 CGCG CG^CG Cac8I 2260 GCGTGC GC[ACGT]^[ACGT]GC EcoRV 3337 GATATC GAT^ATC FspI 2345 TGCGCA TGC^GCA MamI 1419 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 50 CAGCTG C[AC]G^C[GT]G MvnI 2167 CGCG CG^CG NciI 1525 CCGGG CC^[GC]GG NdeI 2156 CATATG CA^TATG NlaIV 197 GGCTCC GG[ACGT]^[ACGT]CC PflMI 3284 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 326 CTGCAG CTGCA^G PvuII 50 CAGCTG CAG^CTG SacI 3098 GAGCTC GAGCT^C SpeI 118 ACTAGT A^CTAGT SspI 292 AATATT AAT^ATT Van91I 3284 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 589 GTATAC GT^[AC][GT]AC 1672 GTATAC AocI 1272 CCTGAGG CC^T[ACGT]AGG 3125 CCTGAGG BclI 839 TGATCA T^GATCA 3351 TGATCA BsaWI 1692 ACCGGA [AT]^CCGG[AT] 1944 ACCGGA Bst1107I 589 GTATAC GTA^TAC 1672 GTATAC BstZ17I 589 GTATAC GTA^TAC 1672 GTATAC Bsu36I 1272 CCTGAGG CC^T[ACGT]AGG 3125 CCTGAGG Eco81I 1272 CCTGAGG CC^T[ACGT]AGG 3125 CCTGAGG EcoT14I 1115 CCTTGG C^C[AT][AT]GG 3407 CCTTGG FbaI 839 TGATCA T^GATCA 3351 TGATCA MaeII 914 ACGT A^CGT 2296 ACGT MwoI 2218 GCAAGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 2949 GCAAACAGAGC NspI 108 GCATGT [GA]CATG^[CT] 705 ACATGT SapI 3031 GAAGAGC GCTCTTC(1/4) 669 GCTCTTC ScaI 3187 AGTACT AGT^ACT 3222 AGTACT SfcI 128 CTATAG C^T[GA][CT]AG 326 CTGCAG StyI 1115 CCTTGG C^C[AT][AT]GG 3407 CCTTGG TaqI 427 TCGA T^CGA 2754 TCGA TthHB8I 427 TCGA T^CGA 2754 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI Asp700I BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI CfoI DdeI DpnII DraI EarI EcoRI EcoRII EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspI MvaI NdeII NlaIII NsiI PleI RsaI Sau3AI ScrFI SfaNI SmlI SspBI StuI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI