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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BsiEI BsmBI BsoBI
BspDI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII
BstPI BstXI BstYI CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I FseI HaeII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2167 CGCG CG^CG
AflII 3363 CTTAAG C^TTAAG
AflIII 705 ACATGT A^C[GA][CT]GT
AspHI 3098 GAGCTC G[AT]GC[AT]^C
AviII 2345 TGCGCA TGC^GCA
BciVI 2963 GTATCC GTATCC(6/5)
BcnI 1525 CCGGG CC^[GC]GG
BfrI 3363 CTTAAG C^TTAAG
BsaBI 1419 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2050 GTGCAG GTGCAG(16/14)
BsiHKAI 3098 GAGCTC G[AT]GC[AT]^C
BsiWI 2293 CGTACG C^GTACG
BspLU11I 705 ACATGT A^CATGT
BstAPI 2218 GCAAGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2167 CGCG CG^CG
Cac8I 2260 GCGTGC GC[ACGT]^[ACGT]GC
EcoRV 3337 GATATC GAT^ATC
FspI 2345 TGCGCA TGC^GCA
MamI 1419 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 50 CAGCTG C[AC]G^C[GT]G
MvnI 2167 CGCG CG^CG
NciI 1525 CCGGG CC^[GC]GG
NdeI 2156 CATATG CA^TATG
NlaIV 197 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 3284 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 326 CTGCAG CTGCA^G
PvuII 50 CAGCTG CAG^CTG
SacI 3098 GAGCTC GAGCT^C
SpeI 118 ACTAGT A^CTAGT
SspI 292 AATATT AAT^ATT
Van91I 3284 CCAAACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 589 GTATAC GT^[AC][GT]AC
1672 GTATAC
AocI 1272 CCTGAGG CC^T[ACGT]AGG
3125 CCTGAGG
BclI 839 TGATCA T^GATCA
3351 TGATCA
BsaWI 1692 ACCGGA [AT]^CCGG[AT]
1944 ACCGGA
Bst1107I 589 GTATAC GTA^TAC
1672 GTATAC
BstZ17I 589 GTATAC GTA^TAC
1672 GTATAC
Bsu36I 1272 CCTGAGG CC^T[ACGT]AGG
3125 CCTGAGG
Eco81I 1272 CCTGAGG CC^T[ACGT]AGG
3125 CCTGAGG
EcoT14I 1115 CCTTGG C^C[AT][AT]GG
3407 CCTTGG
FbaI 839 TGATCA T^GATCA
3351 TGATCA
MaeII 914 ACGT A^CGT
2296 ACGT
MwoI 2218 GCAAGGACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2949 GCAAACAGAGC
NspI 108 GCATGT [GA]CATG^[CT]
705 ACATGT
SapI 3031 GAAGAGC GCTCTTC(1/4)
669 GCTCTTC
ScaI 3187 AGTACT AGT^ACT
3222 AGTACT
SfcI 128 CTATAG C^T[GA][CT]AG
326 CTGCAG
StyI 1115 CCTTGG C^C[AT][AT]GG
3407 CCTTGG
TaqI 427 TCGA T^CGA
2754 TCGA
TthHB8I 427 TCGA T^CGA
2754 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI CfoI
DdeI DpnII DraI EarI EcoRI
EcoRII EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspI MvaI NdeII
NlaIII NsiI PleI RsaI Sau3AI
ScrFI SfaNI SmlI SspBI StuI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI