##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BalI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 65 GGCGTC G[GA]^CG[CT]C
AhdI 2028 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2392 CCTCAGG CC^T[ACGT]AGG
Asp700I 3296 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2028 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1741 CCCGAG C^[CT]CG[GA]G
BamHI 1065 GGATCC G^GATCC
BanI 2397 GGCACC G^G[CT][GA]CC
BglI 2329 GCCCGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2924 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2924 GCTGAGC GC^T[ACGT]AGC
BsaHI 65 GGCGTC G[GA]^CG[CT]C
BseRI 44 CTCCTC GAGGAG(10/8)
BsmBI 1660 CGTCTC CGTCTC(1/5)
BsoBI 1741 CCCGAG C^[CT]CG[GA]G
Bsp1407I 933 TGTACA T^GTACA
BspHI 2668 TCATGA T^CATGA
BsrGI 933 TGTACA T^GTACA
Bst1107I 1037 GTATAC GTA^TAC
BstAPI 2733 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 639 GGTTACC G^GT[ACGT]ACC
BstPI 639 GGTTACC G^GT[ACGT]ACC
BstXI 1439 CCAAACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1037 GTATAC GTA^TAC
Bsu36I 2392 CCTCAGG CC^T[ACGT]AGG
CelII 2924 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2028 GACTAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2392 CCTCAGG CC^T[ACGT]AGG
EcoO65I 639 GGTTACC G^GT[ACGT]ACC
HaeII 1555 AGCGCC [GA]GCGC^[CT]
Hin1I 65 GGCGTC G[GA]^CG[CT]C
HincII 2036 GTCGAC GT[CT]^[GA]AC
HindII 2036 GTCGAC GT[CT]^[GA]AC
HindIII 1052 AAGCTT A^AGCTT
KspI 78 CCGCGG CCGC^GG
NcoI 2985 CCATGG C^CATGG
NdeI 328 CATATG CA^TATG
PflMI 2301 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 788 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 2668 TCATGA T^CATGA
SacII 78 CCGCGG CCGC^GG
SalI 2036 GTCGAC G^TCGAC
SbfI 3021 CCTGCAGG CCTGCA^GG
SmlI 1155 CTCAAG C^T[CT][GA]AG
Sse8387I 3021 CCTGCAGG CCTGCA^GG
SspBI 933 TGTACA T^GTACA
Van91I 2301 CCACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3296 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 1037 GTATAC GT^[AC][GT]AC
2036 GTCGAC
AvaII 789 GGACC G^G[AT]CC
1980 GGTCC
BclI 524 TGATCA T^GATCA
2708 TGATCA
BcnI 145 CCGGG CC^[GC]GG
3004 CCCGG
BspMI 2787 GCAGGT ACCTGC(4/8)
2953 GCAGGT
DsaI 78 CCGCGG C^C[GA][CT]GG
2985 CCATGG
FbaI 524 TGATCA T^GATCA
2708 TGATCA
MaeII 519 ACGT A^CGT
2034 ACGT
NciI 145 CCGGG CC^[GC]GG
3004 CCCGG
PvuII 844 CAGCTG CAG^CTG
2427 CAGCTG
SacI 1834 GAGCTC GAGCT^C
2962 GAGCTC
SphI 1385 GCATGC GCATG^C
1422 GCATGC
StuI 1853 AGGCCT AGG^CCT
2389 AGGCCT
TaqI 443 TCGA T^CGA
2037 TCGA
TthHB8I 443 TCGA T^CGA
2037 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII