################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI AscI AseI AsnI Asp718I AspEI BbrPI BbvCI BcgI BciVI BsaBI BsiEI Bsp1407I BspDI BsrGI BssHII BstBI BstXI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI HpaI KpnI KspI MamI MluI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RsrII SacII SalI SapI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 868 GTATAC GT^[AC][GT]AC AflII 373 CTTAAG C^TTAAG AocI 318 CCTAAGG CC^T[ACGT]AGG Aor51HI 1551 AGCGCT AGC^GCT AspI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2029 TGCGCA TGC^GCA AvrII 3495 CCTAGG C^CTAGG BbeI 2277 GGCGCC GGCGC^C BfrI 373 CTTAAG C^TTAAG BglI 1925 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1626 AGATCT A^GATCT BlnI 3495 CCTAGG C^CTAGG BlpI 3048 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3048 GCTGAGC GC^T[ACGT]AGC BsaAI 199 TACGTG [CT]AC^GT[GA] BsiWI 991 CGTACG C^GTACG BsmI 3078 GAATGC GAATGC(1/-1) BspLU11I 1094 ACATGT A^CATGT BsrBI 2072 GAGCGG CCGCTC(-3/-3) Bst1107I 868 GTATAC GTA^TAC BstEII 54 GGTCACC G^GT[ACGT]ACC BstPI 54 GGTCACC G^GT[ACGT]ACC BstZ17I 868 GTATAC GTA^TAC Bsu36I 318 CCTAAGG CC^T[ACGT]AGG CelII 3048 GCTGAGC GC^T[ACGT]AGC Eco47III 1551 AGCGCT AGC^GCT Eco81I 318 CCTAAGG CC^T[ACGT]AGG EcoO65I 54 GGTCACC G^GT[ACGT]ACC EcoRI 1129 GAATTC G^AATTC FspI 2029 TGCGCA TGC^GCA HincII 463 GTCAAC GT[CT]^[GA]AC HindII 463 GTCAAC GT[CT]^[GA]AC KasI 2277 GGCGCC G^GCGCC MfeI 1948 CAATTG C^AATTG MunI 1948 CAATTG C^AATTG NaeI 328 GCCGGC GCC^GGC NarI 2277 GGCGCC GG^CGCC NgoMIV 328 GCCGGC G^CCGGC NheI 1225 GCTAGC G^CTAGC PflFI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3422 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 989 GACGTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 2233 CCTGCAGG CCTGCA^GG ScaI 2940 AGTACT AGT^ACT SfoI 2277 GGCGCC GGC^GCC SphI 2257 GCATGC GCATG^C Sse8387I 2233 CCTGCAGG CCTGCA^GG StuI 1587 AGGCCT AGG^CCT Tth111I 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3422 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2562 CCATCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 2181 TCCGGA T^CCGGA 2865 TCCGGA AcyI 1654 GGCGTC G[GA]^CG[CT]C 2277 GGCGCC AflIII 200 ACGTGT A^C[GA][CT]GT 1094 ACATGT Alw44I 938 GTGCAC G^TGCAC 2991 GTGCAC ApaI 2309 GGGCCC GGGCC^C 2971 GGGCCC ApaLI 938 GTGCAC G^TGCAC 2991 GTGCAC BalI 806 TGGCCA TGG^CCA 2088 TGGCCA BclI 1903 TGATCA T^GATCA 2022 TGATCA BsaHI 1654 GGCGTC G[GA]^CG[CT]C 2277 GGCGCC BsaI 9 GAGACC GGTCTC(1/5) 2451 GAGACC BseAI 2181 TCCGGA T^CCGGA 2865 TCCGGA BsgI 187 GTGCAG GTGCAG(16/14) 1364 GTGCAG BsmBI 995 CGTCTC CGTCTC(1/5) 2822 GAGACG BspEI 2181 TCCGGA T^CCGGA 2865 TCCGGA BsrDI 539 GCAATG GCAATG(2/0) 1139 GCAATG BsrFI 328 GCCGGC [GA]^CCGG[CT] 743 ACCGGC Cfr10I 328 GCCGGC [GA]^CCGG[CT] 743 ACCGGC DraII 2309 GGGCCCT [GA]G^G[ACGT]CC[CT] 2472 AGGCCCT EarI 3013 CTCTTC CTCTTC(1/4) 1855 GAAGAG EcoO109I 2309 GGGCCCT [GA]G^G[ACGT]CC[CT] 2472 AGGCCCT FbaI 1903 TGATCA T^GATCA 2022 TGATCA Hin1I 1654 GGCGTC G[GA]^CG[CT]C 2277 GGCGCC Ksp632I 3013 CTCTTC CTCTTC(1/4) 1855 GAAGAG MluNI 806 TGGCCA TGG^CCA 2088 TGGCCA MroI 2181 TCCGGA T^CCGGA 2865 TCCGGA MscI 806 TGGCCA TGG^CCA 2088 TGGCCA MslI 2516 CACATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3073 CATCAGAATG NspI 1094 ACATGT [GA]CATG^[CT] 2257 GCATGC PvuII 1379 CAGCTG CAG^CTG 2390 CAGCTG SspI 796 AATATT AAT^ATT 3575 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI