##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BbrPI BbvCI BcgI BciVI BsaBI
BsiEI Bsp1407I BspDI BsrGI BssHII
BstBI BstXI ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI HpaI
KpnI KspI MamI MluI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 868 GTATAC GT^[AC][GT]AC
AflII 373 CTTAAG C^TTAAG
AocI 318 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1551 AGCGCT AGC^GCT
AspI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2029 TGCGCA TGC^GCA
AvrII 3495 CCTAGG C^CTAGG
BbeI 2277 GGCGCC GGCGC^C
BfrI 373 CTTAAG C^TTAAG
BglI 1925 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1626 AGATCT A^GATCT
BlnI 3495 CCTAGG C^CTAGG
BlpI 3048 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3048 GCTGAGC GC^T[ACGT]AGC
BsaAI 199 TACGTG [CT]AC^GT[GA]
BsiWI 991 CGTACG C^GTACG
BsmI 3078 GAATGC GAATGC(1/-1)
BspLU11I 1094 ACATGT A^CATGT
BsrBI 2072 GAGCGG CCGCTC(-3/-3)
Bst1107I 868 GTATAC GTA^TAC
BstEII 54 GGTCACC G^GT[ACGT]ACC
BstPI 54 GGTCACC G^GT[ACGT]ACC
BstZ17I 868 GTATAC GTA^TAC
Bsu36I 318 CCTAAGG CC^T[ACGT]AGG
CelII 3048 GCTGAGC GC^T[ACGT]AGC
Eco47III 1551 AGCGCT AGC^GCT
Eco81I 318 CCTAAGG CC^T[ACGT]AGG
EcoO65I 54 GGTCACC G^GT[ACGT]ACC
EcoRI 1129 GAATTC G^AATTC
FspI 2029 TGCGCA TGC^GCA
HincII 463 GTCAAC GT[CT]^[GA]AC
HindII 463 GTCAAC GT[CT]^[GA]AC
KasI 2277 GGCGCC G^GCGCC
MfeI 1948 CAATTG C^AATTG
MunI 1948 CAATTG C^AATTG
NaeI 328 GCCGGC GCC^GGC
NarI 2277 GGCGCC GG^CGCC
NgoMIV 328 GCCGGC G^CCGGC
NheI 1225 GCTAGC G^CTAGC
PflFI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3422 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 989 GACGTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2233 CCTGCAGG CCTGCA^GG
ScaI 2940 AGTACT AGT^ACT
SfoI 2277 GGCGCC GGC^GCC
SphI 2257 GCATGC GCATG^C
Sse8387I 2233 CCTGCAGG CCTGCA^GG
StuI 1587 AGGCCT AGG^CCT
Tth111I 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3422 CCACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2562 CCATCAATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 2181 TCCGGA T^CCGGA
2865 TCCGGA
AcyI 1654 GGCGTC G[GA]^CG[CT]C
2277 GGCGCC
AflIII 200 ACGTGT A^C[GA][CT]GT
1094 ACATGT
Alw44I 938 GTGCAC G^TGCAC
2991 GTGCAC
ApaI 2309 GGGCCC GGGCC^C
2971 GGGCCC
ApaLI 938 GTGCAC G^TGCAC
2991 GTGCAC
BalI 806 TGGCCA TGG^CCA
2088 TGGCCA
BclI 1903 TGATCA T^GATCA
2022 TGATCA
BsaHI 1654 GGCGTC G[GA]^CG[CT]C
2277 GGCGCC
BsaI 9 GAGACC GGTCTC(1/5)
2451 GAGACC
BseAI 2181 TCCGGA T^CCGGA
2865 TCCGGA
BsgI 187 GTGCAG GTGCAG(16/14)
1364 GTGCAG
BsmBI 995 CGTCTC CGTCTC(1/5)
2822 GAGACG
BspEI 2181 TCCGGA T^CCGGA
2865 TCCGGA
BsrDI 539 GCAATG GCAATG(2/0)
1139 GCAATG
BsrFI 328 GCCGGC [GA]^CCGG[CT]
743 ACCGGC
Cfr10I 328 GCCGGC [GA]^CCGG[CT]
743 ACCGGC
DraII 2309 GGGCCCT [GA]G^G[ACGT]CC[CT]
2472 AGGCCCT
EarI 3013 CTCTTC CTCTTC(1/4)
1855 GAAGAG
EcoO109I 2309 GGGCCCT [GA]G^G[ACGT]CC[CT]
2472 AGGCCCT
FbaI 1903 TGATCA T^GATCA
2022 TGATCA
Hin1I 1654 GGCGTC G[GA]^CG[CT]C
2277 GGCGCC
Ksp632I 3013 CTCTTC CTCTTC(1/4)
1855 GAAGAG
MluNI 806 TGGCCA TGG^CCA
2088 TGGCCA
MroI 2181 TCCGGA T^CCGGA
2865 TCCGGA
MscI 806 TGGCCA TGG^CCA
2088 TGGCCA
MslI 2516 CACATTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3073 CATCAGAATG
NspI 1094 ACATGT [GA]CATG^[CT]
2257 GCATGC
PvuII 1379 CAGCTG CAG^CTG
2390 CAGCTG
SspI 796 AATATT AAT^ATT
3575 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI