##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvrII BbeI BbrPI BcgI BciVI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
FseI HgaI Hin1I HindIII HpaI
KasI KpnI MaeII MluI MroI
NarI NdeI NheI NotI NruI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflII 337 CTTAAG C^TTAAG
Alw44I 2814 GTGCAC G^TGCAC
ApaLI 2814 GTGCAC G^TGCAC
AseI 2642 ATTAAT AT^TAAT
AsnI 2642 ATTAAT AT^TAAT
AviII 2513 TGCGCA TGC^GCA
BamHI 1102 GGATCC G^GATCC
BbvCI 2095 GCTGAGG CCTCAGC(-5/-2)
BcnI 1527 CCGGG CC^[GC]GG
BfrI 337 CTTAAG C^TTAAG
BsaBI 1990 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2881 GCATTC GAATGC(1/-1)
BspHI 1998 TCATGA T^CATGA
BspMI 2424 ACCTGC ACCTGC(4/8)
DraII 53 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 53 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 1012 GATATC GAT^ATC
EcoT22I 2307 ATGCAT ATGCA^T
FspI 2513 TGCGCA TGC^GCA
HaeII 1798 GGCGCT [GA]GCGC^[CT]
HincII 1309 GTTGAC GT[CT]^[GA]AC
HindII 1309 GTTGAC GT[CT]^[GA]AC
KspI 58 CCGCGG CCGC^GG
MamI 1990 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1000 CAATTG C^AATTG
MunI 1000 CAATTG C^AATTG
NaeI 27 GCCGGC GCC^GGC
NciI 1527 CCGGG CC^[GC]GG
NgoMIV 27 GCCGGC G^CCGGC
NsiI 2307 ATGCAT ATGCA^T
PacI 2376 TTAATTAA TTAAT^TAA
PflMI 2004 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 53 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1771 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2642 ATTAAT AT^TAAT
RcaI 1998 TCATGA T^CATGA
SacII 58 CCGCGG CCGC^GG
SbfI 1113 CCTGCAGG CCTGCA^GG
Sse8387I 1113 CCTGCAGG CCTGCA^GG
StuI 3083 AGGCCT AGG^CCT
TaqI 1160 TCGA T^CGA
TthHB8I 1160 TCGA T^CGA
Van91I 2004 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1743 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 266 GTCTAC GT^[AC][GT]AC
3047 GTCTAC
AccII 20 CGCG CG^CG
59 CGCG
AflIII 2051 ACATGT A^C[GA][CT]GT
3053 ACATGT
AlwNI 2005 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
2503 CAGGTGCTG
BalI 89 TGGCCA TGG^CCA
368 TGGCCA
BanI 772 GGCACC G^G[CT][GA]CC
3200 GGCACC
BanII 69 GAGCTC G[GA]GC[CT]^C
1751 GAGCTC
BsgI 1225 CTGCAC GTGCAG(16/14)
682 GTGCAG
BspLU11I 2051 ACATGT A^CATGT
3053 ACATGT
BsrDI 1053 CATTGC GCAATG(2/0)
1842 CATTGC
BsrFI 27 GCCGGC [GA]^CCGG[CT]
1473 ACCGGC
BstF5I 2706 GGATG GGATG(2/0)
3127 GGATG
BstUI 20 CGCG CG^CG
59 CGCG
BstYI 912 AGATCC [GA]^GATC[CT]
1102 GGATCC
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
1473 ACCGGC
DraI 2131 TTTAAA TTT^AAA
3191 TTTAAA
EcoRI 1171 GAATTC G^AATTC
2579 GAATTC
FauI 57 CCCGC CCCGC(4/6)
1667 CCCGC
FokI 2706 GGATG GGATG(9/13)
3127 GGATG
MflI 912 AGATCC [GA]^GATC[CT]
1102 GGATCC
MluNI 89 TGGCCA TGG^CCA
368 TGGCCA
MscI 89 TGGCCA TGG^CCA
368 TGGCCA
MvnI 20 CGCG CG^CG
59 CGCG
NcoI 331 CCATGG C^CATGG
952 CCATGG
PstI 245 CTGCAG CTGCA^G
1114 CTGCAG
PvuII 248 CAGCTG CAG^CTG
783 CAGCTG
SacI 69 GAGCTC GAGCT^C
1751 GAGCTC
SmlI 337 CTTAAG C^T[CT][GA]AG
3302 CTCAAG
XhoII 912 AGATCC [GA]^GATC[CT]
1102 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII DrdI DsaI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI