##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AvrII BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SbfI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 784 GGTACC G^GTACC
AccI 2033 GTCGAC GT^[AC][GT]AC
AflIII 2612 ACACGT A^C[GA][CT]GT
Alw44I 851 GTGCAC G^TGCAC
Aor51HI 1429 AGCGCT AGC^GCT
ApaLI 851 GTGCAC G^TGCAC
Asp718I 784 GGTACC G^GTACC
AspI 1779 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 221 CCCGAG C^[CT]CG[GA]G
AviII 1186 TGCGCA TGC^GCA
BglII 288 AGATCT A^GATCT
BlpI 1197 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1197 GCTAAGC GC^T[ACGT]AGC
BsiWI 1445 CGTACG C^GTACG
BsmBI 2873 CGTCTC CGTCTC(1/5)
BsoBI 221 CCCGAG C^[CT]CG[GA]G
BspHI 2632 TCATGA T^CATGA
BsrDI 1454 CATTGC GCAATG(2/0)
BsrFI 2399 ACCGGC [GA]^CCGG[CT]
BssHII 2516 GCGCGC G^CGCGC
CelII 1197 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2399 ACCGGC [GA]^CCGG[CT]
DrdI 737 GACCAACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1429 AGCGCT AGC^GCT
EcoRI 16 GAATTC G^AATTC
FspI 1186 TGCGCA TGC^GCA
HincII 2033 GTCGAC GT[CT]^[GA]AC
HindII 2033 GTCGAC GT[CT]^[GA]AC
KpnI 784 GGTACC GGTAC^C
NheI 2820 GCTAGC G^CTAGC
NspI 1346 GCATGC [GA]CATG^[CT]
PflFI 1779 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2031 GAGTC GAGTC(4/5)
RcaI 2632 TCATGA T^CATGA
SalI 2033 GTCGAC G^TCGAC
SapI 1019 GAAGAGC GCTCTTC(1/4)
ScaI 3147 AGTACT AGT^ACT
SexAI 855 ACCAGGT A^CC[AT]GGT
SfiI 2393 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1346 GCATGC GCATG^C
SspI 1638 AATATT AAT^ATT
TfiI 1988 GATTC G^A[AT]TC
Tth111I 1779 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
ApaI 336 GGGCCC GGGCC^C
1696 GGGCCC
AspHI 672 GAGCAC G[AT]GC[AT]^C
851 GTGCAC
BamHI 1176 GGATCC G^GATCC
1435 GGATCC
BbeI 1598 GGCGCC GGCGC^C
2215 GGCGCC
BsiHKAI 672 GAGCAC G[AT]GC[AT]^C
851 GTGCAC
BstEII 98 GGTGACC G^GT[ACGT]ACC
228 GGTGACC
BstPI 98 GGTGACC G^GT[ACGT]ACC
228 GGTGACC
DraIII 143 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
854 CACCAGGTG
EarI 2303 CTCTTC CTCTTC(1/4)
1019 GAAGAG
EcoO65I 98 GGTGACC G^GT[ACGT]ACC
228 GGTGACC
HinfI 1988 GATTC G^A[ACGT]TC
2031 GAGTC
KasI 1598 GGCGCC G^GCGCC
2215 GGCGCC
Ksp632I 2303 CTCTTC CTCTTC(1/4)
1019 GAAGAG
KspI 1313 CCGCGG CCGC^GG
2546 CCGCGG
MseI 2564 TTAA T^TAA
2668 TTAA
NarI 1598 GGCGCC GG^CGCC
2215 GGCGCC
SacII 1313 CCGCGG CCGC^GG
2546 CCGCGG
SfoI 1598 GGCGCC GGC^GCC
2215 GGCGCC
Tru9I 2564 TTAA T^TAA
2668 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII