##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BclI BglI BlnI
BsaAI BsaHI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsoBI BspDI BspEI
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflII 1853 CTTAAG C^TTAAG
AseI 1410 ATTAAT AT^TAAT
AsnI 1410 ATTAAT AT^TAAT
BalI 92 TGGCCA TGG^CCA
BbsI 714 GTCTTC GAAGAC(2/6)
BcgI 743 CGAGCAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1853 CTTAAG C^TTAAG
BlpI 213 GCTGAGC GC^T[ACGT]AGC
BmyI 442 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 213 GCTGAGC GC^T[ACGT]AGC
BpuAI 714 GTCTTC GAAGAC(2/6)
BsaBI 916 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1650 GAGACC GGTCTC(1/5)
BseRI 68 GAGGAG GAGGAG(10/8)
BsiEI 862 CGGCCG CG[GA][CT]^CG
Bsp1286I 442 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 944 TGTACA T^GTACA
BspHI 704 TCATGA T^CATGA
BsrDI 684 CATTGC GCAATG(2/0)
BsrGI 944 TGTACA T^GTACA
BstXI 512 CCACGGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 213 GCTGAGC GC^T[ACGT]AGC
EagI 862 CGGCCG C^GGCCG
EclXI 862 CGGCCG C^GGCCG
Eco52I 862 CGGCCG C^GGCCG
EcoNI 1191 CCTTAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 366 ATGCAT ATGCA^T
FauI 17 CCCGC CCCGC(4/6)
HgaI 259 GCGTC GACGC(5/10)
HindIII 1515 AAGCTT A^AGCTT
MaeII 868 ACGT A^CGT
MamI 916 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 92 TGGCCA TGG^CCA
MscI 92 TGGCCA TGG^CCA
MwoI 41 GCTTTGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 32 CCATGG C^CATGG
NdeI 465 CATATG CA^TATG
NsiI 366 ATGCAT ATGCA^T
PshBI 1410 ATTAAT AT^TAAT
PstI 906 CTGCAG CTGCA^G
RcaI 704 TCATGA T^CATGA
SspBI 944 TGTACA T^GTACA
SwaI 1861 ATTTAAAT ATTT^AAAT
XbaI 2462 TCTAGA T^CTAGA
XcmI 2072 CCATATAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 632 ACATGT A^C[GA][CT]GT
2192 ACATGT
AlwNI 665 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
787 CAGGAACTG
Asp700I 567 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
993 GAAAACCTTC
BciVI 1760 GGATAC GTATCC(6/5)
921 GTATCC
BcnI 113 CCGGG CC^[GC]GG
577 CCCGG
BglII 527 AGATCT A^GATCT
2718 AGATCT
BpmI 26 CTCCAG CTGGAG(16/14)
601 CTGGAG
BsmI 46 GAATGC GAATGC(1/-1)
567 GAATGC
BspLU11I 632 ACATGT A^CATGT
2192 ACATGT
BstYI 527 AGATCT [GA]^GATC[CT]
2718 AGATCT
EarI 2352 CTCTTC CTCTTC(1/4)
2466 GAAGAG
HapII 113 CCGG C^CGG
578 CCGG
HpaI 2125 GTTAAC GTT^AAC
2152 GTTAAC
HpaII 113 CCGG C^CGG
578 CCGG
Ksp632I 2352 CTCTTC CTCTTC(1/4)
2466 GAAGAG
MflI 527 AGATCT [GA]^GATC[CT]
2718 AGATCT
MspI 113 CCGG C^CGG
578 CCGG
NciI 113 CCGGG CC^[GC]GG
577 CCCGG
PvuII 898 CAGCTG CAG^CTG
1319 CAGCTG
XhoII 527 AGATCT [GA]^GATC[CT]
2718 AGATCT
XmnI 567 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
993 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BfaI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoO109I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI