##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AviII AvrII BcgI
BfrI BlnI BsgI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraIII Eco47III Eco81I EcoO65I
FseI FspI HpaI KpnI KspI
MfeI MluI MunI NdeI NheI
NotI NruI PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SbfI ScaI SfcI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1331 GTCGAC GT^[AC][GT]AC
AhdI 52 GACCGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 452 GTGCAC G^TGCAC
ApaLI 452 GTGCAC G^TGCAC
Asp700I 2386 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 52 GACCGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 452 GTGCAC G[AT]GC[AT]^C
BalI 1035 TGGCCA TGG^CCA
BanII 2102 GAGCCC G[GA]GC[CT]^C
BbvCI 1591 GCTGAGG CCTCAGC(-5/-2)
BciVI 2544 GTATCC GTATCC(6/5)
BglI 3198 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1239 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1239 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 452 GTGCAC G[AT]GC[AT]^C
BspMI 2531 ACCTGC ACCTGC(4/8)
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BssSI 3450 CTCGTG CACGAG(-5/-1)
BstBI 366 TTCGAA TT^CGAA
BstXI 1245 CCAACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1239 GCTCAGC GC^T[ACGT]AGC
DraI 620 TTTAAA TTT^AAA
DrdI 2646 GACTGGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2811 CGGCCG C^GGCCG
Eam1105I 52 GACCGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2811 CGGCCG C^GGCCG
Eco52I 2811 CGGCCG C^GGCCG
EcoRI 369 GAATTC G^AATTC
EcoT22I 3138 ATGCAT ATGCA^T
HindIII 2693 AAGCTT A^AGCTT
MluNI 1035 TGGCCA TGG^CCA
MscI 1035 TGGCCA TGG^CCA
NaeI 2957 GCCGGC GCC^GGC
NcoI 1458 CCATGG C^CATGG
NgoMIV 2957 GCCGGC G^CCGGC
NsiI 3138 ATGCAT ATGCA^T
NspV 366 TTCGAA TT^CGAA
PflMI 2982 CCAACTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 1331 GTCGAC G^TCGAC
SapI 1811 GAAGAGC GCTCTTC(1/4)
SfiI 3197 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 366 TTCGAA TT^CGAA
SspI 2289 AATATT AAT^ATT
Van91I 2982 CCAACTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1878 CCAGGATGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2386 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 915 TCCGGA T^CCGGA
1644 TCCGGA
AcyI 1268 GGCGCC G[GA]^CG[CT]C
2944 GGCGCC
AflIII 2757 ACGTGT A^C[GA][CT]GT
3104 ACGTGT
BbeI 1268 GGCGCC GGCGC^C
2944 GGCGCC
BbrPI 1948 CACGTG CAC^GTG
3103 CACGTG
BglII 1770 AGATCT A^GATCT
2409 AGATCT
BsaAI 1948 CACGTG [CT]AC^GT[GA]
3103 CACGTG
BsaHI 1268 GGCGCC G[GA]^CG[CT]C
2944 GGCGCC
BsaI 1511 GAGACC GGTCTC(1/5)
2444 GAGACC
BsaWI 915 TCCGGA [AT]^CCGG[AT]
1644 TCCGGA
BseAI 915 TCCGGA T^CCGGA
1644 TCCGGA
BsmI 3140 GCATTC GAATGC(1/-1)
3437 GCATTC
BspEI 915 TCCGGA T^CCGGA
1644 TCCGGA
BspHI 213 TCATGA T^CATGA
391 TCATGA
BsrDI 337 CATTGC GCAATG(2/0)
2142 GCAATG
EcoNI 2001 CCTTACTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2770 CCTGTCTGAGG
EcoRV 209 GATATC GAT^ATC
2255 GATATC
Hin1I 1268 GGCGCC G[GA]^CG[CT]C
2944 GGCGCC
HincII 1331 GTCGAC GT[CT]^[GA]AC
2060 GTCAAC
HindII 1331 GTCGAC GT[CT]^[GA]AC
2060 GTCAAC
KasI 1268 GGCGCC G^GCGCC
2944 GGCGCC
MroI 915 TCCGGA T^CCGGA
1644 TCCGGA
NarI 1268 GGCGCC GG^CGCC
2944 GGCGCC
NspI 993 GCATGT [GA]CATG^[CT]
1542 ACATGC
PmaCI 1948 CACGTG CAC^GTG
3103 CACGTG
PmlI 1948 CACGTG CAC^GTG
3103 CACGTG
PvuII 968 CAGCTG CAG^CTG
2591 CAGCTG
RcaI 213 TCATGA T^CATGA
391 TCATGA
SexAI 147 ACCAGGT A^CC[AT]GGT
3092 ACCTGGT
SfoI 1268 GGCGCC GGC^GCC
2944 GGCGCC
StuI 1138 AGGCCT AGG^CCT
3196 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspI AvaI
AvaII BamHI BanI BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmaCI XmaI